1. Immunophenotypes of eosinophilic cells in type 2 intrahepatic cholangiocarcinoma
Materials and Methods
We selected 15 cases of type 2 intrahepatic cholangiocarcinoma, which were rich in polygonal eosinophilic cells, and investigated immnophenotypes of these eosinophilic cells.
Results
The eosinophilic cells never showed positive reactivity to Arginase-1 or Hep Par-1. At least 2 of 3 biliary markers, cytokeratin 7, cytokeratin 19 and CA19-9, were positive in all cases. Positivity rates of each antibody and figures of representative cases are presented below.
Antibody
Clone
Positive rate
Arginase-1
SP156 (Abcam)
0/15 (0%)
Hep Par-1
OCH1E5 (DAKO)
Cytokeratin 7
OV-TL 12/30 (DAKO)
15/15 (100%)
Cytokeratin 19
RCK108 (DAKO)
14/15 (93.3%)
CA19-9
1116-NS-19-9 (DAKO)
10/15 (66.7%)
Arginase-1 (-)
Hep Par-1 (-)
Cytokeratin 7 (+)
Cytokeratin 19 (+)
CA19-9 (+)
Cytokeratin (+)
CA19-9 (-)
Arginase-1
Hep Par-1
Cytokeratin 7
Cytokeratin 19
CA19-9
Case 1
Case 2

2. Case 3 Case 4 Case 5 Arginase-1 (-) Hep Par-1 (-) Cytokeratin 7 (+)
CA19-9 (focal +)
Arginase-1
Hep Par-1
Cytokeratin 7
Cytokeratin 19
CA19-9
Case 3
Case 4
Case 5
Non-neoplastic hepatocytes
Scale bars represent 100 μm.