Presentation is loading. Please wait.

Presentation is loading. Please wait.

Electrical Muscle Stimulation Induces an Increase of VEGFR2 on Circulating Hematopoietic Stem Cells in Patients With Diabetes  Asa Hidmark, PhD, Ioannis.

Published byFranklin Snow Modified over 7 years ago

Similar presentations

Presentation on theme: "Electrical Muscle Stimulation Induces an Increase of VEGFR2 on Circulating Hematopoietic Stem Cells in Patients With Diabetes  Asa Hidmark, PhD, Ioannis."— Presentation transcript:

1 Electrical Muscle Stimulation Induces an Increase of VEGFR2 on Circulating Hematopoietic Stem Cells in Patients With Diabetes  Asa Hidmark, PhD, Ioannis Spanidis, Thomas H. Fleming, PhD, Nadine Volk, PhD, Volker Eckstein, PhD, Jan B. Groener, PhD, Stefan Kopf, PhD, Peter P. Nawroth, Prof. Dr. Med, Dr. H.C., Dimitrios Oikonomou, PhD  Clinical Therapeutics  Volume 39, Issue 6, Pages e2 (June 2017) DOI: /j.clinthera Copyright © 2017 The Authors Terms and Conditions

2 Figure 1 EMS treatment resulted in an immediate and transient reduction in the frequency of HSCs in blood. (A) Representative flow cytometric dot plot of CD34+ HSCs in leukocytes versus forward scatter (FSC). (B) Frequency of HSCs in circulating leukocytes in 9 healthy control volunteers (white boxes) and in patients with diabetes (black circles) before the first EMS treatment (n = 27) and in patients with diabetes before the fourth EMS treatment (n = 22). (Statistical error analysis was performed with 2-tailed Mann-Whitney U test.) (C) Change in frequency of HSCs between samples taken immediately before and after the first EMS treatment of 9 healthy control volunteers (white box) and 27 patients with diabetes (gray box). (D) Change in mean fluorescence intensity (MFI) of CD34 on HSCs of patients with diabetes after the first EMS treatment (n = 27). Boxes indicate 25th to 75th percentile, whiskers indicate 5th to 95th percentile, and the line indicates median. Statistical error analysis was performed with 2-tailed Wilcoxon׳s signed-rank test. EMS = electric muscle stimulation; HSC = hematopoietic stem cell; PBMC = peripheral blood mononuclear cell; PE = phycoerythrin. Clinical Therapeutics  , e2DOI: ( /j.clinthera ) Copyright © 2017 The Authors Terms and Conditions

3 Figure 2 JAM-A and CXCR4 were up-regulated on the surface of HSCs in circulation after EMS treatment of patients with diabetes. (A) Representative flow cytometric dot plot of JAM-A and CXCR4 on HSCs in PBMC. Percentage of change was calculated between the baseline value (before first EMS) and after first EMS (white boxes) or before fourth EMS (gray boxes): change in frequency of HSCs expressing (B) CXCR4, (C) JAM-A (1 outlier excluded from after first treatment and before fourth treatment) or (D) CD31. n = 9. Boxes indicate 25th to 75th percentile, whiskers indicate 5th to 95th percentile, and line indicates median. Statistical error analysis was performed with 2-tailed Wilcoxon׳s signed-rank test. APC = allophycocyanin; CXCR4 = C-X-C chemokine receptor type 4; EMS = electric muscle stimulation; FITC = fluorescein isothiocyanate; HSC = hematopoietic stem cell; JAM-A = junctional adhesion molecule A; PBMC = peripheral blood mononuclear cell. Clinical Therapeutics  , e2DOI: ( /j.clinthera ) Copyright © 2017 The Authors Terms and Conditions

4 Figure 3 Functional markers of EPC activation were increased on HSCs in EMS-treated patients with diabetes. Percentages of change were calculated between samples taken immediately before and after the first EMS treatment (white boxes) or before the fourth EMS treatment (gray boxes). (A) Change in frequency of HSCs expressing VEGFR2 (ie, frequency of EPCs; 1 outlier excluded before the fourth treatment). (B) Change in frequency of VEGFR2+ on CXCR4+ HSCs. (C) Changes in mean fluorescence intensity (MFI) of CD31 on EPCs. (D) Change in size as measured by forward scatter (FSC) of EPCs. n = 9. Boxes indicate 25th to 75th percentile, whiskers indicate 5th to 95th percentile, and line indicates median. Statistical error analysis was performed with 2-tailed Wilcoxon׳s signed-rank test. CXCR4 = C-X-C chemokine receptor type 4; EMS = electric muscle stimulation; EPC = endothelial progenitor cell; HSC = hematopoietic stem cell; VEGFR2 = vascular endothelial growth factor receptor 2. Clinical Therapeutics  , e2DOI: ( /j.clinthera ) Copyright © 2017 The Authors Terms and Conditions

5 Figure 4 EMS treatment, but not sham treatment, caused an increased frequency of EPCs among the HSCs. Six of the healthy volunteers were recruited to receive a sham treatment according to standard protocol for EMS. Blood was drawn before sham, after sham, and after EMS treatments. Red blood cells were lysed, and leukocytes were immediately stained with anti-CD34 and anti-VEGFR2. Boxes indicate 25th to 75th percentile, whiskers indicate 5th to 95th percentile, and line indicates median. Statistical error analysis was performed with 2-tailed Wilcoxon׳s signed-rank test. EMS = electric muscle stimulation; EPC = endothelial progenitor cell; HSC = hematopoietic stem cell; VEGFR2 = vascular endothelial growth factor receptor 2. Clinical Therapeutics  , e2DOI: ( /j.clinthera ) Copyright © 2017 The Authors Terms and Conditions

6 Figure S1 EMS-treatment of diabetic patients did not significantly alter. plasma anti-oxidative capacity. Plasma from diabetes patients immediately before and after the first EMS-treatment was used to measure antioxidant capacity (Boxes show percentile, whiskers show 5-95 percentile, line indicates median) (n=9) (a), or methylglyoxal (bars show average, error bars show standard deviation) (n=10) (b). Measurement after treatment is depicted in grey bars. Statistical error analysis was performed with 2-tailed Wilcoxon׳s signed ranks test. ns=not significant. Clinical Therapeutics  , e2DOI: ( /j.clinthera ) Copyright © 2017 The Authors Terms and Conditions

7 Figure S2 Expression of endothelial adhesion molecule CD31 and JAMA increased in EMS-treated individuals with diabetes. Changes of surface expression on HSC between samples taken immediately before and after 1st the first EMS (white boxes) or before 4th EMS (grey boxes): Changes in Mean Fluorescence Intensity (MFI) of surface expression of JAM-A (1 outlier excluded from before 4th treatment) (a) or CD31 (b). Change of MFI of CD31 on CXCR4+ HSC (c) or on JAM-A+ HSC (d). Per cent change is calculated between the baseline value (Before 1st EMS) and After 1st EMS or Before 4th EMS. Boxes show percentile, whiskers show 5-95 percentile, line indicates median. Statistical error analysis was performed with 2-tailed Wilcoxon׳s signed ranks test. Clinical Therapeutics  , e2DOI: ( /j.clinthera ) Copyright © 2017 The Authors Terms and Conditions

Download ppt "Electrical Muscle Stimulation Induces an Increase of VEGFR2 on Circulating Hematopoietic Stem Cells in Patients With Diabetes  Asa Hidmark, PhD, Ioannis."

Similar presentations

A. a). d). c). f). b).e). g). Figure S1. NAT2 expression in lymphocytes and monocytes from a healthy volunteer. PBMC from a healthy volunteer were simple.

A. a). d). c). f). b).e). g). Figure S1. NAT2 expression in lymphocytes and monocytes from a healthy volunteer. PBMC from a healthy volunteer were simple.
Figure e-1A.

Figure e-1A.
Alterations in blood leukocytes of G551D-bearing cystic fibrosis patients undergoing treatment with ivacaftor  Preston E. Bratcher, Steven M. Rowe, Ginger.

Alterations in blood leukocytes of G551D-bearing cystic fibrosis patients undergoing treatment with ivacaftor  Preston E. Bratcher, Steven M. Rowe, Ginger.
CD4+CD25+ regulatory T cells suppress contact hypersensitivity reactions through a CD39, adenosine-dependent mechanism  Sabine Ring, PhD, Stephen J. Oliver,

CD4+CD25+ regulatory T cells suppress contact hypersensitivity reactions through a CD39, adenosine-dependent mechanism  Sabine Ring, PhD, Stephen J. Oliver,
Human group 2 innate lymphoid cells do not express the IL-5 receptor

Human group 2 innate lymphoid cells do not express the IL-5 receptor
IFN-γ and STAT1 are required for efficient induction of CXC chemokine receptor 3 (CXCR3) on CD4+ but not CD8+ T cells by Joseph Barbi, Steve Oghumu, Claudio.

IFN-γ and STAT1 are required for efficient induction of CXC chemokine receptor 3 (CXCR3) on CD4+ but not CD8+ T cells by Joseph Barbi, Steve Oghumu, Claudio.
Regulatory T cells as a biomarker for response to adalimumab in rheumatoid arthritis  Dao X. Nguyen, BSc, Alice Cotton, RN, BSc, Laura Attipoe, MBBS, Coziana.

Regulatory T cells as a biomarker for response to adalimumab in rheumatoid arthritis  Dao X. Nguyen, BSc, Alice Cotton, RN, BSc, Laura Attipoe, MBBS, Coziana.
Intact interferon-γ response against Coxiella burnetii by peripheral blood mononuclear cells in chronic Q fever  T. Schoffelen, J. Textoris, C.P. Bleeker-Rovers,

Intact interferon-γ response against Coxiella burnetii by peripheral blood mononuclear cells in chronic Q fever  T. Schoffelen, J. Textoris, C.P. Bleeker-Rovers,
Reduced Frequency of Regulatory T Cells in Peripheral Blood Stem Cell Compared to Bone Marrow Transplantations  Céline Blache, Joe-Marc Chauvin, Aude.

Reduced Frequency of Regulatory T Cells in Peripheral Blood Stem Cell Compared to Bone Marrow Transplantations  Céline Blache, Joe-Marc Chauvin, Aude.
Correlation of allergen-specific T follicular helper cell counts with specific IgE levels and efficacy of allergen immunotherapy  Yin Yao, MD, Cai-Ling.

Correlation of allergen-specific T follicular helper cell counts with specific IgE levels and efficacy of allergen immunotherapy  Yin Yao, MD, Cai-Ling.
Monocyte chemoattractant chemokines in cystic fibrosis

Monocyte chemoattractant chemokines in cystic fibrosis
Reduced TH1/TH17 CD4 T-cell numbers are associated with impaired purified protein derivative–specific cytokine responses in patients with HIV-1 infection 

Reduced TH1/TH17 CD4 T-cell numbers are associated with impaired purified protein derivative–specific cytokine responses in patients with HIV-1 infection 
CD154 Expression Is Associated with Neutralizing Antibody Titer Levels Postinfluenza Vaccination in Stem Cell Transplant Patients and Healthy Adults 

CD154 Expression Is Associated with Neutralizing Antibody Titer Levels Postinfluenza Vaccination in Stem Cell Transplant Patients and Healthy Adults 
Pathways Responsible for Human Autoantibody and Therapeutic Intravenous IgG Activity in Humanized Mice  Inessa Schwab, Anja Lux, Falk Nimmerjahn  Cell.

Pathways Responsible for Human Autoantibody and Therapeutic Intravenous IgG Activity in Humanized Mice  Inessa Schwab, Anja Lux, Falk Nimmerjahn  Cell.
Volume 141, Issue 4, Pages e6 (October 2011)

Volume 141, Issue 4, Pages e6 (October 2011)
Eosinophils from Hematopoietic Stem Cell Recipients Suppress Allogeneic T Cell Proliferation  Jennie Andersson, Julia Cromvik, Madeleine Ingelsten, Christine.

Eosinophils from Hematopoietic Stem Cell Recipients Suppress Allogeneic T Cell Proliferation  Jennie Andersson, Julia Cromvik, Madeleine Ingelsten, Christine.
Impaired function of human T-lymphotropic virus type 1 (HTLV-1)–specific CD8+ T cells in HTLV-1–associated neurologic disease by Amir H. Sabouri, Koichiro.

Impaired function of human T-lymphotropic virus type 1 (HTLV-1)–specific CD8+ T cells in HTLV-1–associated neurologic disease by Amir H. Sabouri, Koichiro.
Probiotic supplementation has little effect on peripheral blood regulatory T cells  Nicholas P. West, PhD, Peggy L. Horn, PhD, David B. Pyne, PhD, Hilary.

Probiotic supplementation has little effect on peripheral blood regulatory T cells  Nicholas P. West, PhD, Peggy L. Horn, PhD, David B. Pyne, PhD, Hilary.
Apoptosis Susceptibility Prolongs the Lack of Memory B Cells in Acute Leukemic Patients After Allogeneic Hematopoietic Stem Cell Transplantation  Angela.

Apoptosis Susceptibility Prolongs the Lack of Memory B Cells in Acute Leukemic Patients After Allogeneic Hematopoietic Stem Cell Transplantation  Angela.
Altered Homeostasis of CD4+ Memory T Cells in Allogeneic Hematopoietic Stem Cell Transplant Recipients: Chronic Graft-versus-Host Disease Enhances T Cell.

Altered Homeostasis of CD4+ Memory T Cells in Allogeneic Hematopoietic Stem Cell Transplant Recipients: Chronic Graft-versus-Host Disease Enhances T Cell.

Similar presentations

Ads by Google