1. The core facility platform at the Biomedical Centre
Sandra jovic

2. The platform overview Located at BMC
Largest bioscience centre in Scandinavia
1300 researchers
Wide range of techniques
Open to internal and external users

3. The platform overview Immunohistochemistry BiaCore Bio-Plex
RT-PCR (up-and-coming)
Sample preparation for:
Scanning electron microscopy (SEM)
Transmission electron microscopy (TEM)
Delphi microscope
IVIS
Me (Sandra Jovic)
Maria Baumgarten
Artur Schmidtchen

4. Workflow Immunohistochemistry Tissue sample
Processing (dehydrate/embed)
Sectioning
Antigen retrieval
Immunohistochemistry

5. Instruments Microtome Leica Microtome RM2255
Sectioning of paraffin-embedded specimens
4 µm

6. Instruments PT Link Dako PT Link
Reducing the number of operations needed in the pre-treatment process
Delayed start

7. Instruments Autostainer Dako Autostainer 48 Up to 48 slides per run
Tracking all slides
Slides treated in the same way

8. Instruments BiaCore BiaCore X100 Study molecular interactions SPR
Changes in the refractive index are detected and recorded as RU
Terminology (ligand/analyte)

9. Instruments BiaCore Covalent immobilization
High affinity capture
CM-chips (carboxymethylated dextran)
SA-chips (treptavidin)
NTA-chips (nitrilotriacetic)

10. Instruments Bio-Plex Bio-Plex 200
Investigation of up to 50 analytes in a small sample volume
ELISA/flow cytometery
Cell culture supernatants, plasma, serum, lavage, sputum
Discounts

11. Instruments Real-Time PCR QuantStudio 7 Flex Real-Time PCR System
Amplification/detection of nucleic acids
Gene expression, miRNA profiling, SNP genotyping, pathogen detection

12. Instruments IVIS IVIS Spectrum instrument
Monitoring of distribution of bio-luminescent or florescent biomarkers
Real-time
Smaller animals

13. Contact Info
Sandra Jovic, Ph.D
Division of Infection medicine, Department of Clinical Sciences, Lund University

14. Methods/Techniques Sample prepartion for TEM/SEM
Transmission Electron Microscope (TEM)
Negative staining
Gold labelling
Scanning Electron Microscope (SEM)
Metal coating
Delphi microscope

15. Instruments Delphi DELMIC and Phenom-World
Combined fluorescence and electron microscopy
Detailed structural information with nanoscale resolution

16. Instruments Critical point dryer Baltec CPD 03
Dries biological specimens
Exchanges water/alcohol with liquid carbon dioxide

17. Instruments Ultramicrotome Reichert UltraCut S ultramicrotome
0 to 2,5 microns
60 nm
High quality ultra-thin sections for electron microscopy and light microscopy

18. Instruments Fluorescence microscope
Olympus IX73 and BX43 fluorescence microscope
Coupled to the ultramicrotome
Used for preparation of various specimens for electron microscopical analyses

19. Instruments Automatic contrasting instrument
Leica EM AC20
Contrasting with heavy metal

20. Instruments Sputter coater Leica ACE 200
Producing homogenous and conductive metal or carbon coatings for SEM and TEM analysis

21. Contact Info
Maria Baumgarten, Biomedical scientist
Division of Infection medicine, Department of Clinical Sciences, Lund University