1. WP 4 – Valorisation of algal biomass
Second Consortium Meeting
13-14 June
Stockholm, Sweden

2. WP4: Valorisation of Algae Biomass
Introduction: WP4
WP4: Valorisation of Algae Biomass
Biomass Harvesting: IBET
Biomass Refinement: Extractis
Animal Feed: PMIX
Coatings and Adhesives: INSP
Fillers and pastes: INSTM/POLIMI

3. Milestones and Deliverables

4. Milestones and Deliverables
MS5 Optimal pre-concentration by membrane ultrafiltration and low shear centrifugation iBET M16 sept-17
MS6 Choice of the best biomass refinement process Extr M16 sept-17
MS7 Decision on scale up demonstration activities for polymer compounding, pastes and coatings INSTM M23 apr-18
MS8 Valorisation activities: polymer compounding, pastes, coatings and animal feed feedstocks PMIX M36 may-19
Deliverables
D4.1 Systems design for harvesting and refinement and algae characterisation iBET M14 jul-17
D4.2 Biomass valorisation report – feasibility Extr M20 jan-18
D4.3 Biomass valorisation progress report – first generation products INSP M28 sept-18
D4.4 Final biomass valorisation report (animal feed, resins and residues) INSTM M36 may-19

5. iBET

6. Extractis

7. Objectives Deliverables Milestones M16
Task 4. 2 Biomass refinement and extraction
Objectives
Efficiency of the pre-treatment (DM in the sludge around 20%)
Fractionate algae biomass into three fractions consisting in respectively oily phase, aqueous phase and sludge with, at least, 80% purity of the oily phase and protein content above 65% of the protein fraction.
Purity of beta-carotene (≥ 80%) and digestibility of the protein phase equivalent to soy concentrate.
Deliverables
D4.2 Biomass valorisation report - Feasibility (M20)
Milestones M16
Choice of the best biomass refinement process
Verification :
- 80% purity of the oily phase
- Protein content above 65%
- Purity of beta-carotene (≥ 80%).

8. Description of actions and progress
Task 4. 2 Biomass refinement and extraction
Description of actions and progress

9. Task 4.2 Biomass refinement and extraction
Last results

10. Task 4.2 Biomass refinement and extraction
Sample
Algae
Characterizations
Yields 7
Archimede Spirulina (HPH +pH11)
DM : 98,9%
Ash (%/DM) :22.6
Proteins (%/DM) : 35.7 ND 8
Archimede Spirulina (pH4)
DM : 99,6%
Ash (%/DM) : 3.4
Proteins (%/DM) : 72.2
Solubilisation yield (Prot supernatant 1 / prot algae)= 59%
Global yield (prot powder/prot Algae) = 26% 9
 Archimede Tetraselmis (HPH+pH11)
DM : 99,9%
Ash (%/DM) : 16.9
Proteins (%/DM) : 20.6 10
 Archimede Tetraselmis (pH4)
DM : 99,5%
Ash (%/DM) : 4.21
Proteins (%/DM) : 56.2
Solubilisation yield (Prot supernatant 1 / prot algae)= ND
Global yield (prot powder/prot Algae) = 40%
2 phases, no lipidic phase
Last characterizations in progress : free and total sugars, lipids

11. Open issues Timing plan Requirements from
Task Biomass refinement and extraction
Open issues
Regarding the quantities of lipids requested by PRODUMIX (6kg of lipids which correspond to 300 kg of biomass):
at the moment, we are not able to extract lipids.
Even if the combination HPH or Ultrasounds pretreatment followed by a chemical treatment is efficient for the proteins extraction, it’s not the case for the lipids.
- Will a third approach be necessary?
- Will the demo sites be able to provide such fresh quantities to EXTRACTIS?
Timing plan
- Optimization: - Proteins solubility curves for each algae realized previously
- Determination of the best acidic and alcaline pH conditions
- Increase the purity of the proteins fraction
- Extraction of lipids by optimizing the HPH parameters (number of passes, number of stages…)
Requirements from
- DEMO SITEs (ARAVA, KOTO and ARCHIMEDE) : algae

12. PMIX

13. INSP

14. INSTM

15. Introduction: Our role
WP4.5: High value material fillers & pastes
4.5a: Algae-residue characterization and modification
4.5b: Development of algae-based biocomposites
4.5c: Development of algae-based 3D-printing of ceramic materials and liquid pastes

16. Introduction: Chemical process

17. Microscopy

18. TGA analysis Spirulina Tetraselmis ~17.0% final weight

19. Microscopy – Spirulina 5%
Pre-heat
800°C
50x
100x

20. Microscopy – Tetraselmis 5%
Pre-heat
800°C
50x
100x

21. Mechanical properties

22. Chemically reactive part
Composition
Process modifiers
Chemically reactive part

23. Compression Test

24. Compression Test
Empty cylinder samples
3D printed
15 mm
7.5 mm
25 mm

25. Compression Test – Printed Pipes: time evolution

26. TGA analysis Spirulina Tetraselmis ~17.0% final weight

27. Compression Test – Printed Pipes: heating

28. Ball milling

29. Compression Test – Printed Pipes: milling
24h milling

30. Compression Test
Milling 24h
Printing
Heating 800°C

31. Compression Test – Printed Pipes: heating and milling
24h milling

32. Next steps

33. Next steps
Start 4.5b: Development of algae-based biocomposites with natural rubber and possibly other polymers
Complete the last mechanical tests
Validate the results with algae fractions

34. Thank you for your kind attention