1. Paper-Based Diagnostic Device
Team Members: Ben Hagarty (MSE), Nick Dudak (MSE), Qi Sun (EE), Jingxuan Sun (EE) – May 14-26a
Advisers/Clients: Dr. Santosh Pandey (EE), Dr. Rebecca Cademartiri (MSE), Dr. Ludovico Cademartiri (MSE)
Paper pH Testing
– pH Hydrion
Salivary Amylase
– Yamaguchi
Problem Statement:
Paper-based pH test kits currently include only a single type of test which limits the value the kit provides to the customer
Current State of the Market:
Create a multi-functional paper-based test kit for human saliva to test pH and amylase to give a preliminary indication of disease.
Solution
Intended Users and Uses
Those people especially at risk from problems caused by poor dental hygiene such as in developing countries or the impoverished
Those with genetic predisposition to oral disease
Those in high stress environments
Those at risk of schizophrenia
Design Requirements
Functional
Multifunctional device 
5 mL of volume used 
No power needed 
Colorimetric output 
One-time use
Non-Functional
Paper substrate 
Portable 
Quick, easy, and safe to use 
Relatively inexpensive (~$5 )
Kit Components
Saliva collector
Test strips
Paper substrate with wax barriers and lamination
Test chemicals pre-deposited
Indicator for pH ; Substrate for Amylase
Color Reference Strip
D. Smartphone App
Based on iOS
Image color
comparison
Testing Kit Instructions for Use
1. Dip strip into saliva
3. Compare to reference strip or use app to measure value
2. Remove and wait for color to change
Design Requirements
Amylase was chosen due to relatively high levels in human saliva. 
Biomarker for several medical issues
Pancreatitis, mumps, renal failure
Pre-diagnostic tool, not final diagnosis
Proof of concept for other paper-based salivary diagnostics
Amylase Overview
Color Detector App Description
Operating System: iOS®
Language: Objective C®
Created by X-code®: a suite of software development tools for Apple devices
The app is able to determine the color produced by test via an iPhone® photo
This color is then compared to reference values to determine the test result
Color Detection App Instructions
3a) Visual readout by User or
3b) Digital Readout with iPhone® Application
Design Approach
Test Strip Construction
1. Design of several strips made
2. Printed with wax printer and heated to melt through
4. Chemicals for each test are applied
3. Lamination is applied to front and back
Acknowledgements
Zach Njus, Isaac Johns, Suhuai Song, Dr. Meng Lu – Dept. of Electrical Engineering; Dr. Joseph Burnett, Dept. of Chemistry