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Library # ESTs and Descriptions

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1 Library # ESTs and Descriptions
Bacterial infected leaf Flower bud Anther Fruit, early fruit Hair root Early root Placenta Pytophthora capsici infected plant Fruit,immature: cm(+seeds) Fruit, mature green: pericarp(-seeds) Fruit, breaker: pericarp(-seeds) Fruit, mature red ripe: pericarp(-seeds) Seeds of friuts with placenta Flower bud, size 3~ 8mm Open flower Bark Peduncle Callus/ regeneration Germination stage: 4 & 6 day Germination stage: 18 day rbcS-silenced leaves 9749 Capsicum annuum Capsicum chinense Capsicum frutescens Capsicum baccatum Capsicum chacoense Capsicum pubescens Capsicum eximium Capsicum ciliatum Capsicum cardenasii Capsicum galapagoense Capsicum lycianthoides Capsicum tovarii Capsicum flexuosum Capsicum spp.

2 Pepper Chip: Custom Content
Capsicum ESTs 123,489 Capsicum genomic DNA 466 Capsicum mRNAs 515 COS markers 642 30,815 unigenes

3 Chip Design Array Format: 49 Feature Size: 5 µm
Custom content:    6,415,642 probes/30,815 unigenes Background probes:    33,886 antigenomic (surrogate MM) probes Bacterial Expression sets: 81 probe sets including bioB, bioC, bioD from E. coli and cre from P1 Bacteriophage and Dap, lys, phe, thr, trp from B. subtilis Technical Replicate Controls: 100 probes organized into 10 sets of 10 probes each tiled 169 times SNP training set

4 Distribution of probes per sequence
Mean =210 probes/unigene

5 Pepper Microarray Consortium
PIs: Allen Van Deynze, Alex Kozik, Jiquiang Yao and Jim Prince Collaborators: Molly Jahn, Shanna Moore, Doil Choi                                             zx


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