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Published byMarian Bridges Modified over 7 years ago
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Microarray data collection for different stresses
Data curation and processing INtegrative Meta-analysis of EXpression data –INMEX - Integrity check for all the datasets Combined analysis of all the datasets Comparative analysis between each datasets Stouffer's method (based on inverse normal transformation). Stouffer's method incorporates weight (i.e. based on sample sizes) into the calculation; They usually have very similar performance. However, in microarray meta-analysis, larger sample size does not warrant larger weights as the quality of each study can be variable. Stouffer's method of analysis at significance level – 0.05 Up- and down-regulated gene list ID conversions to rene ontology IDs Selection of common genes Classification of genes using Blast2GO qPCR for top 15 up and down regulated genes
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Figure S2. Flow chart depicting stepwise protocol followed to identify the shared genes in different individually occurring biotic and abiotic stresses. The processed or raw transcriptome data were collected from the publically available database and curated files in .csv format were integrated using INMEX [ to identify commonly upregulated genes. Further the upregulated genes were classified using Blast2GO [ The commonly (shared) regulated top 15 up- and down-regulated genes were identified based on INMEX output data.
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