2. A novel green technology for the removal of biofilm
Content:
Introduction Rentacs
About Legionella
Dutch legislation on legionella prevention
Removing biofilm using patented technology
Step-by-step plan for the cleaning of a (drinking) water installation
Demo Cleaning and results LabAqua Valencia

3. Introduction Rentacs Rentacs : Rent Aqua Cleaning Solutions
Founded in 2014 to introduce patented cleaning technique to Europe.
Rentacs Int. is represented by Mrs. S. Vreeswijk.
Rentacs Nl is represented by mr. M van Zeijl.
Rentacs NL performs 4-5 cleaning projects a week for several different customers, such as the Dutch Army and government.
Rentacs INT focusses on sales and development of cleaning units and cooperative business development in Europe with partners.

4. About Legionella
Symptoms of a legionella infection

5. About Legionella
Statistics Temperatures

6. About Legionella
Statistics of Legionella source and outbreak

7. Dutch legislation on legionella prevention
Risk analysis
A number of businesses and institutions are subject to stricter regulations on legionella prevention. If you own a hotel, public swimming pool, camp site or marina, for example, you must engage a certified company to conduct a risk analysis and draw up a legionella management plan of your drinking water installation

8. Dutch legislation on legionella prevention
Water samples and check valves.
This plan describes the periodical preventive checks and the maintenance of your water installations. You must check the water (or have it checked) at least twice a year.
If the standard of 1,000 colony-forming units per liter is exceeded, you must report this to the Human Environment and Transport Inspectorate (Inspectie Leefomgeving en Transport, ILT). And disinfect.
Flushing the lines every week and registration in a logbook on location.

9. Dutch legislation on legionella prevention
Because legionella can be everwhere

10. Removing biofilm using patented technology

11. Removing biofilm using patented technology
Step 1: The entire installation is inspected, before a cleaning operation, with a view to charting whether all the tap points can be accessed by one of the employees.
Step 2: The safety coordinator of the company cleaning team will ensure that all the people present at the location are aware of the work that they will carry out during the cleaning. The people present are informed about the possible dangers involved.
Step 3: All the tap points that cannot be guaranteed to be unused are made inaccessible before the cleaning is started.
Step 4: The entire water installation is thermally treated using water at a temperature of at least 70 degrees Celsius.
Step 5: At the same time air bubbles are regularly entrained along with the hot water.
Step 6: The installation is filled with a pesticide permitted by the CTB (Board for the Authorisation of Pesticides). This is then allowed to soak in for the required time. 
Step 7: Thermal treatment with water of at least 70 degrees Celsius.
Step 8: Thermal treatment with air bubbles.
Step 9: Rinsing with cold mains water until the pesticide has been completely removed from the installation.
Step 10: All the tap points in the installation are checked for the absence of the pesticides used.
Step 11: Any temporary modifications to the installation that are required for the cleaning are removed and/or restored.
Step 12: The cleaning project manager releases the drinking water for use. If there was a high standard value for Legionella before the cleaning, the atomising tap points will not be released.

12. Step-by-step plan for the cleaning of a (drinking) water installation
The entire installation is inspected, before a cleaning operation, with a view to charting whether all the tap points can be accessed by one of the employees.

13. Step-by-step plan for the cleaning of a (drinking) water installation
The safety coordinator of the company cleaning team will ensure that all the people present at the location are aware of the work that they will carry out during the cleaning. The people present are informed about the possible dangers involved.
Step 3:
All the tap points that cannot be guaranteed to be unused are made inaccessible before the cleaning is started.

14. Step-by-step plan for the cleaning of a (drinking) water installation
The entire water installation is thermally treated using water at a temperature of at least 70 degrees Celsius.

15. Step-by-step plan for the cleaning of a (drinking) water installation
At the same time air bubbles are regularly entrained along with the hot water.

16. Step-by-step plan for the cleaning of a (drinking) water installation
The installation is filled with a pesticide permitted by the CTB (College Toelating Bestrijdingsmiddelen, Board for the Authorisation of Pesticides).
This is then allowed to soak in for the required time. 

17. Step-by-step plan for the cleaning of a (drinking) water installation
Thermal treatment with water of at least 70 degrees Celsius.
Step 8:
Thermal treatment with air bubbles.
Step 9:
Rinsing with cold mains water until the pesticide has been completely removed from the installation.

18. Step-by-step plan for the cleaning of a (drinking) water installation
All the tap points in the installation are checked for the absence of the pesticides used.

19. Step-by-step plan for the cleaning of a (drinking) water installation
Any temporary modifications to the installation that are required for the cleaning are removed and/or restored.
Step 12:
The cleaning project manager releases the drinking water for use. If there was a high standard value for Legionella before the cleaning, the atomising tap points will not be released.

20. Step-by-step plan for the cleaning of a (drinking) water installation

21. ACS 1.0 Max temp 85 degrees Power 4,5 Kw
Use 5 tap points at the same time for cleaning
Chemical: All lines
Thermal: Copper, RVS
and Iron (with plastic
we need to ask if it is
possible to heat it up
until 85 degrees)

22. ACS 2.0 Max temp 85 degrees Power 6,5 Kw
Use 10 tap points at the same time for cleaning
Chemical: All lines
Thermal: Copper, RVS
and Iron (with plastic
we need to ask if it is
possible to heat it up
until 85 degrees)

23. ACS 2.0 Max temp 85 degrees Power 8,8 Kw
Use 20 tap points at the same time for cleaning.
Chemical: All lines
Thermal: Copper, RVS
and Iron (with plastic
we need to ask if it is
possible to heat it up
until 85 degrees)

24. Test by Labaqua in Valencia
1. Description test in a net that has previous legionella problems.
2.1.- Choose of water Facility.
2.2.- Test GANT diagram.
2.3.- Test scheme proposed.
2. Results
3.1.- Results in test water tubes
3.2.- Mesophilic bacteriums culture 37 ºC results.

25. Test by Labaqua in Valencia
2.1. Choose of water Facility
Customer
ALFAFAR CITY HALL
Facility description
Cold and hot water in all the building (dressing rooms).
Water source
Municipality water net.
Water tanks
Don´t exist.
Number and kind of water heating tanks
4 Heat water tanks, no purge.
Number and kind of final water points
4 Dressing rooms 25 Showers and 16 taps

26. Test by Labaqua in Valencia
2.2. Test GANT diagram
SEPTEMBER 2015
OCTOBER 2015
NOVEMBER 2015
Spike of biofilm in dockable water tubes
System installation
Preparation of dockable water tubes
Biofilm growth in dockable water tubes
System spike (20th of July)
Instalation
Critical point (CP1) : Biofilm growth detected
Biofilm medium preparation
Biofilm Growth

27. Test by Labaqua in Valencia
2.2. Test GANT diagram
SEPTEMBER 2015
OCTOBER 2015
NOVEMBER 2015
Preparation of tech test
We choose a facility contaminated with legionella, similar to Oliva Golf Hotel in Barcelona or Valence.
Cleaning and disinfection of the facility and dockable water tubes (Dockable water tubes will be coupled in a extreme tap or shower)
CP2: Search of Building contaminated with Legionella
Rentacs preparation visit
Tech test
Test

28. Test by Labaqua in Valencia
2.2. Test GANT diagram
SEPTEMBER 2015
OCTOBER 2015
NOVEMBER 2015
Study of Labaqua
Study of biofilm using PCR and visual inspection. Several Samples taken when biofilm start to grow, three, two and one day before test and test day.
Several legionella samples taken before and after the test.
Studies of biofilm in tubes surfaces
Studies of Legionella in selected facility

29. Test by Labaqua in Valencia
2.3. Test scheme proposed.
Alfafar football field legionella elimination test diagram
Security systems witness
Antireturn valve
Key
Rentacs Spike
Alfafar net
Test water tube

30. Test water tubes 1: Without Rentacs treatment.
Test by Labaqua in Valencia
3.1. Results in test water tubes
Test water tubes 1: Without Rentacs treatment.
Test water tubes 2 : Water tubes coupled to a water facility and treated with standard chlorine disinfection system.
Test water tubes 3 : Water tubes coupled to a water facility and treated with Rentacs.

31. T1 (Before water treatment) Control (After chlorine treatment)
Test by Labaqua in Valencia
3.1. Results in test water tubes
T1 (Before water treatment)
Mesophilic B. 22ºC
Mesophilic B. 37ºC
Legionella culture
Legionella qPCR
Copper water tubes
34x103 /mL
35x103 /mL
7600 ufc/sample + flora
61999 UG/2.5 mL
PPR water tubes
63x104 /mL
37x104/mL
Interfering Flora
UG/2.5 mL
Copper WT (Full sample)
34x104
35x104
UG/sample
PPR WT (Full sample)
63x105
37x105
UG/sample
Control (After chlorine treatment)
Mesophilic B. 22ºC
Mesophilic B. 37ºC
Legionella culture
Legionella qPCR
Copper water tubes
45x10
64x10
20600 ufc/sample
40513 UG/2.5 mL
PPR water tubes
93x102
10x102
Interfering Flora
UG/2.5 mL
Copper WT (Full sample)
45x102
64x102
UG/sample
PPR WT (Full sample)
93x103
10x103
UG/sample 10
Non detected
<640 UG/sample

32. T2 (After Rentacs water treatment)
Test by Labaqua in Valencia
3.1. Results in test water tubes
T2 (After Rentacs water treatment)
Mesophilic B. 22ºC
Mesophilic B. 37ºC
Legionella culture
Legionella qPCR
Copper water tubes 1
Non detected
16534 UG/2.5 mL
PPR water tubes
<160 UG/2.5 mL
Copper WT (Full sample) 10
66136 UG/sample
PPR WT (Full sample)
<640 UG/sample
MESOPHILIC B. 37 ºC
Without Water T.
Clorine treatment
Rentacs Treatment
Copper
35x103 /mL
64x10 1
PPR
37x104/mL
10x102
LEGIONELLA CULTURE
Without Water T.
Clorine treatment
Rentacs Treatment
Copper
7600 ufc/muestra + flora
20600 ufc/muestra
Non detected
PPR
Interferent Flora

33. LEGIONELLA PCR (UG/2.5 ml) COPPER/PPR
Test by Labaqua in Valencia
3.1. Results in test water tubes
RESULTS OF LEGIONELLA PCR IN TEST WATER TUBES
Blanco
Cloro
Rentacs
Cobre
61999 UG/2.5 mL
40513 UG/2.5 mL
16534 UG/2.5 mL
PPR
UG/2.5 mL
UG/2.5 mL
<160 UG/2.5 mL
LEGIONELLA PCR (UG/2.5 ml) COPPER/PPR

34. Test by Labaqua in Valencia
3.2.- Mesophilic bacteriums culture 37 ºC results
P. 2: Copper WT after standard chlorination.
P. 1: Copper WT without treatment.
P.3: Copper WT after Rentacs.
P. 1: PPR WT without treatment. .
P. 2: PPR WT after standard chlorination. .
P. 3: PPR WT after Rentacs..

35. Innovations - Vacuum technique - Cooling towers innovations
- Air canals disinfection
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