1. Telephone  512 433 6177  email  info@altogenlabs.com
Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery & Therapeutics
Services > In Vivo Xenograft Services
Xenografting
Xenografting is the transplantation of tissue from one species into another.
Xenografting has been established as benchmark studies in pre-clinical cancer research.
Typically, immunodeficient mice serve as hosts for a wide variety of human tumors, effectively serving as models for human subjects.
Xenografting a complete and accurate study of tumor growth and the activity of drug administration.
Xenografting tumor cells into mice has advanced pre-clinical cancer research significantly
Altogen Labs  Manchaca Road #203  Austin  TX   USA
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2. Telephone  512 433 6177  email  info@altogenlabs.com
Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery & Therapeutics
Services > In Vivo Xenograft Services
Advantages of Xenografting
Develop new therapeutic agents quickly, efficiently and cost effectively
Evaluate the efficacy and toxicity of potential therapeutic agents
Evaluate target compound activity using in vivo system (human cells)
Predict cytotoxicity of cancer drugs
Xenotransplantation is the transplantation of living cells, tissues or organs from one species to another.
Altogen Labs  Manchaca Road #203  Austin  TX   USA
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3. Telephone  512 433 6177  email  info@altogenlabs.com
Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery & Therapeutics
Services > In Vivo Xenograft Services
Xenograft Study Pipelines
Pipeline Xenograft Assay I
Our staff designs the study based on your project requirements and objectives. Leveraging years of knowledge and experience, Altogen Labs guarantees an optimized experimental approach with respect to mice selection, cell lines, inoculation, and dosage.
Pipeline Xenograft Assay II
Testing a Compound
The client provides a compound and cell line to be researched. Our staff will establish a xenograft animal model and test compound activity and how it affects tumor growth.
Pipeline Xenograft Assay III
Testing a Cell Line
The client provides a cell line and our staff tests its ability to establish tumor growth in standard immunodeficient mice.
Representative data of a xenograft compound test study
Nature (2010) Sep 30;467(7315):596-9
Nature. 2010 Sep 30;467(7315):596-9
Altogen Labs  Manchaca Road #203  Austin  TX   USA
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4. Telephone  512 433 6177  email  info@altogenlabs.com
Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery & Therapeutics
Services > In Vivo Xenograft Services
Compound Testing Objectives
How does the test compound affect tumor growth?
Does the test compound inhibit tumor invasion or metastasis?
Localization of test compound in the tumor?
Define drug characteristics:
Bioavailability
Toxicity
Efficacy
Potency
Is the cell line tumorigenic?
Changes in gene expression upon treatment or gene manipulation
How does gene manipulation in a cell line tumor growth, invasiveness, and metastasis
Xenograft studies can rigorously characterize candidate drug efficacy and pharmacokinetic properties
Altogen Labs  Manchaca Road #203  Austin  TX   USA
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5. Telephone  512 433 6177  email  info@altogenlabs.com
Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery & Therapeutics
Services > In Vivo Xenograft Services
Basic Xenograft Study
Xenograft studies can be designed to:
Identify lead compounds
Optimize dose schedules
Identify combination strategies
Altogen Labs  Manchaca Road #203  Austin  TX   USA
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6. Telephone  512 433 6177  email  info@altogenlabs.com
Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery & Therapeutics
Services > In Vivo Xenograft Services
Xenograft Service Options
LNCap cells
MCF-7 cells
Use in-house or customer-provided cell lines
150 in-house cell lines available
Begin testing a compound immediately after injecting mice with tumor cells (unstaged) or once the tumors reach a certain size (staged)
Assign mice to groups randomly or stratify them by body weight, tumor size, disease factors, or any combination of parameters
Administer test compounds by any of the following methods: topically, IP, IV, SC, PO, high pressure tail vein, osmotic minipumps (SC or IP), SC drug pellets, or in the food or water
Snap freeze harvested tumors or fix them for histology or other specialized assays
Analyze blood chemistry at various times throughout the study
jax.org
pathpedia.com
Altogen Labs  Manchaca Road #203  Austin  TX   USA
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7. Telephone  512 433 6177  email  info@altogenlabs.com
Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery & Therapeutics
Services > In Vivo Xenograft Services
Growth Rates for Different Cell Lines
Altogen Labs  Manchaca Road #203  Austin  TX   USA
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8. Telephone  512 433 6177  email  info@altogenlabs.com
Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery & Therapeutics
Services > In Vivo Xenograft Services
Sample Cell Lines Available
Cell Line
Tissue
Disease
Caki-2
Kidney
Clear Cell Carcinoma
PANC-1
Pancreas
Epithiloid Carcinoma
SK-MEL-2
Skin
Malignant Melanoma
SK-OV-3
Ovary
Adenocarcinoma
U-87 MG
Brain
Glioblastoma/Astrocytoma
A431
Epidermis
Epidermoid Carcinoma
SW-480
Colon
HeLa
Cervix
Altogen Labs has ~150 cell lines available for xenograft animal studies Complete listing available at altogenlabs.com/xenograft-models
Altogen Labs  Manchaca Road #203  Austin  TX   USA
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9. Telephone  512 433 6177  email  info@altogenlabs.com
Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery & Therapeutics
Services > In Vivo Xenograft Services
Drug Administration Variables
Route of dose:
Intratumoral
Intramuscular
Oral
Intravenous
Intratracheal
Subcutaneous
Intraperitoneal
Frequency of dose
Duration of dose
Several routes of drug administration can be explored in a Xenograft model
Altogen Labs  Manchaca Road #203  Austin  TX   USA
Telephone   

10. Telephone  512 433 6177  email  info@altogenlabs.com
Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery & Therapeutics
Services > In Vivo Xenograft Services
Data Endpoints
Estimated tumor volume (L x W2 /2)
Mean/median time to specified tumor volume
Survival time to specified tumor volume
Tumor doubling time
Tumor growth inhibition (TGI)
Tumor growth delay (TGD)
Increased life span (ILS)
Tumor cell kill rate
A549 (Lung Carcinoma) Xenograft Study
Altogen Labs  Manchaca Road #203  Austin  TX   USA
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11. Telephone  512 433 6177  email  info@altogenlabs.com
Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery & Therapeutics
Services > In Vivo Xenograft Services
Expanded Xenograft Services
Clinical observations daily
Haematology analysis (blood cell count)
Clinical chemistry profile (of urine and plasma)
Assessment of metastasis
Characterization of the tumor (necrosis, etc)
Tumoral tissue will be banked and preserved for further analysis
Cryo-preservation (for cell culture)
Formalin fixed tumors and tissues (for histopathological and immunostaining analysis)
RNAlater fixed tumors and tissues (for RNA isolation and gene expression analysis)
Our services include expanded tumor cell characterization and preservation
cryostuff2.com
Altogen Labs  Manchaca Road #203  Austin  TX   USA
Telephone   

12. Services > In Vivo Xenograft Services
Provider of Global Contract Research Services Accelerating Preclinical Research, Drug Discovery & Therapeutics
Services > In Vivo Xenograft Services
Altogen Labs’ team of scientists carries extensive experience in Xenograft model research
Altogen Labs is compliant as a Good Laboratory Practices (GLP) environment, which is required by the FDA for for pre-clinical research.
Our Altogen Labs’ Xenograft services are extensive, rigorous, and flexible.
Altogen Labs can partner with you for any Xenograft research project.
Photo credit: wisegeek.com
Contact us to discuss Xenograft model details, timeline estimates, and price quotes!
Altogen Labs  Manchaca Road #203  Austin  TX   USA
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