1. Prof. Pavle R. Andjus, PhD Center for laser microscopy; Institute for physiology and biochemistry Faculty of Biology University of Belgrade, Belgrade SERBIA
Expertise in:
Culturing of primary rodent neural cells (neurons, astrocytes, microglia).
Immunofluorescence analysis of biomolecular markers in neural cells and tissue slices on confocal microscope.
Ca2+ and ROS imaging on cells in vitro.
Patch clamp electrophysiology.
Magnetic resonance imaging of rodents (MRI clinical setups, novel Bruker EPR for in vivo imaging)
Animal models: ALS G93A rat, rat model of stroke (MCAO), tenascin C KO mice
Availability of dental stem cells from the apical papilla (SCAPs) - could be differentiated to neurons and glia
Availability of graphene in different states as a nanomaterial for cell plating, growth and differentiation.

2. Expected benefits and activities during participation in BIONECA:
Share experience with groups who use stem cells for brain diseases (e.g. cell transfection, improved tracing protocols, the modulation of inflammation, modulation of synaptogenesis, etc)
Would like to learn from groups who are experienced in image analyses and post-processing (eg IMARIS or Stereo Investigator software szstems)
Would like to share experience in new imaging modalities – nonlinear microscopy, soft and hard X ray microscopy , Micro CT, Holotomography
Woudl like to propose applications for high resolution microscopy.
Would like to learn about and propose design of new biomaterials for combined application with stem cells for brain diseases
Would like to benefit from modeling to adjust protocols for manipulation with stem cells
Would like to gather innovative combination of partners for new projects` applications
Foreseen maximum contribution: WG4 with partial interest in WG2 .