1. Skimune™ Technologies for sensitization and potency assessment   Unique tools for the Cosmetic and Chemical Industries.  

2. Skin Explant Technology Skimune TM technology
Blood Donor
Immature Mo-DC´s
Mature DC´s + compound
+ T Cells
Sensitized T cells +
Skin biopsy
- Response
+ Response

3. Histopathological evaluation
Grade I
Grade II
Grade III
Grade IV
in vitro tissue damage can predict in vivo tissue damage, tissue damage graded I-IV
(Lerner, K.G., et al., Transplant Proc, (4): p )
Grade 0 = normal skin
Grade I = mild vacuolization of epidermal cells
Grade II = diffuse vacuolization of basal cells with scattered dyskeratotic bodies
Grade III = sub-epidermal cleft formation
Grade IV = complete epidermal separation

4. SkimuneTM assay What it models:
Damaged skin
The sensitization and elicitation phases of the skin sensitization process. Adapted from (
The SkimuneTM model models the in vivo acute contact dermatitis (ACD) model

5. 95% specificity and 95% sensitivity, concordance 95%
Alcyomics
Assay evaluation
SkimuneTM – LLNA
The SkimuneTM assay was evaluated against the mouse local lymph node assay (LLNA), which is the European gold standard for sensitization testing
Number of positive LLNA chemicals = SkimuneTM prediction of positive chemicals = 19
Number of negative LLNA chemicals = SkimuneTM prediction of negative chemicals = 19
95% specificity and 95% sensitivity, concordance 95%

6. SkimuneTM technologies detect human reactions
Reactions to Nickel Sulphate ( NiSO4) cannot be picked up using mouse models
of toxicology-mouse physiology is not human physiology !!

7. Potency assessment
Potency assessment of cinnamic alcohol and oxazalone.
Skin explant results show histopathological damage in skin in response to 0.1µM, 0.05µM and 0.01µM concentration of each chemical. For Cinnamic alcohol 0.1µM gave a grade III positive response, 0.05µM and 0.01µM gave a grade I negative response. Oxazalone gave a grade III positive response at 0.1µM and 0.05µM and a grade I negative response at 0.01µM. Results show oxazalone is a more potent chemical than cinnamic alcohol

8. Case study 1- Cosmeceuticals
Determine the effect of repeated applications of product X in skin
Positive control
Product X Day2
Product X Day3
Negative control
Result:
Repeated application of
Product X over a 3 day period
showed an increase in damage.
Results show a grade II/III
positive response on day 2 and
a grade III positive response
on day 3.
Results indicate that product
X has the potential to cause
an adverse response.

9. Case study 2- Cosmeceuticals
Determine in-vitro responses to wheat protein products
Medium Grade I
Compound 1 Grade I
Compound 2Grade III
Two wheat protein products were tested for immunogenic responses. Compound 2 gave a positive response showing a high degree of histological damage indicating that is likely to cause an adverse reaction. Compound 1 showed no damage response in skin histology

10. www.alcyomics.com Contact: anne.dickinson@alcyomics.com