1. In the name of God

2. Presented by: Dr. Siavash M. Shanehsaz
Department of Dermatology Afzalipour Hospital Kerman Medical School
Pathology
Presented by: Dr. Siavash M. Shanehsaz

4. 22-Treponemal Diseases
VENEREAL SYPHILIS

5. VENEREAL SYPHILIS Histopathology
The two fundamental pathologic changes in syphilis are (a) swelling and proliferation of endothelial cells and (b) a perivascular infiltrate of lymphoid cells and often of plasma cells.
In late secondary and tertiary syphilis, there are also granulomatous infiltrates comprising epithelioid histiocytes and giant cells.
Newborns with congenital syphilis have shown at autopsy multiorgan involvement by an angioinvasive CD68+ mononuclear cell infiltrate that imparts an "onion-skin" morphology to involved vessels with numerous demonstrable spirochetes.

6. 22-Treponemal Diseases
Primary Syphilis

7. Histopathology
At the periphery: acanthosis, spongiosis and exocytosis of lymphocytes and neutrophils. Toward the center: thinned, edematous and permeated by inflammatory cells.
Papillary dermis is edematous.
A dense perivascular and interstitial lymphohistiocytic and plasmacellular infiltration.
Endarteritis obliterans characterized by endothelial swelling and mural edema is observed.
By silver staining with the Levaditi stain or the Warthin-Starry stain and by immunofluorescent techniques, spirochetes are usually identified.

8. 22-Treponemal Diseases
Secondary Syphilis

9. Histopathology
Psoriasiform hyperplasia, often with spongiosis and basilar vacuolar alteration.
Exocytosis of lymphocytes, spongiform pustulation, and parakeratosis also may be observed.
Marked papillary dermal edema and a perivascular and/or periadnexal infiltration.
Vascular changes such as endothelial swelling and mural edema accompany the angiocentric infiltrates in half of the cases.
There are several histologic variants of secondary syphilis-namely, condylomata lata, syphilitic alopecia, pustular lesions, syphilis cornee, and lues maligna.

10. Secondary Syphilis Histopathology
Lesions of condylomata lata show more florid epithelial hyperplasia and intraepithelial microabscess formation.
Syphilitic alopecia demonstrates a superficial and deep perivascular and perifollicular lymphocytic and plasmacellular infiltration.
Lues maligna is an ulcerative form characterized by severe thrombotic endarteritis obliterans involving vessels at the dermal-subcutaneous junction with resultant ischemic necrosis.
Syphilis cornee / keratoderma punctatum associated with secondary syphilis manifests an epidermal invagination with a moderately dense perivascular plasmacellular infiltration.
In the rare pustular lesions of secondary syphilis, a necrotizing pustular follicular reaction accompanied by noncaseating granulomata and a perivascular lymphoplasmacellular infiltration.

11. 22-Treponemal Diseases
Tertiary Syphilis

12. Tertiary Syphilis Histopathology
Tertiary syphilis is categorized into nodular tertiary syphilis confined to the skin; benign gummatous syphilis principally affecting skin, bone, and liver; cardiovascular syphilis; syphilitic hepatic cirrhosis; and neurosyphilis.
In the first variant: the granulomas are small and may be absent in rare cases. The granulomatous process is limited to the dermis with scattered islands of epithelioid cells admixed with a few multinucleated giant cells, lymphocytes and plasma cells.
In cutaneous lesions of benign gummatous syphilis: the blood vessels throughout the dermis and subcutaneous fat exhibit endarteritis obliterans along with angiocentric plasmacellular infiltrates of variable density. The skin lesions involve the subcutaneous fat as well as the dermis.

14. NON VENEREAL TREPONEMATOSES Yaws (Frambesia Tropica)
22-Treponemal Diseases
NON VENEREAL TREPONEMATOSES
Yaws (Frambesia Tropica)

15. Histopathology
Primary lesions: show acanthosis, papillomatosis,spongiosis, and neutrophilic exocytosis with intraepidermal microabscess formation. A heavy, diffuse, dermal infiltrate of plasma cells, lymphocytes, histiocytes, and granulocytes is observed.
Secondary lesions: show the same histologic appearance, resembling condylomata lata in their epidermal changes but differing by virtue of the dermal infiltrate being in a diffuse, as opposed to a perivascular, disposition.
The ulcerative lesions of tertiary yaws: greatly resemble those observed in late syphilis in histologic appearance.

16. 22-Treponemal Diseases
Pinta

17. Histopathology
Pinta
Primary and secondary lesions: show a similar morphology-namely, acanthosis with spongiosis and a sparse dermal infiltrate of lymphocytes, plasma cells, and neutrophils disposed about dilated blood vessels. Increased numbers of Langerhans cells are present in the epidermis.
Lesions of the tertiary stage: are hyperpigmented, characterized by a large number of melanophages within the dermis, or depigmented, manifesting complete absence of epidermal melanin.

18. Erythema Chronicum Migrans
22-Treponemal Diseases
Lyme Disease
Erythema Chronicum Migrans

19. Histopathology
An intense superficial and deep angiocentric, neurotropic, and eccrinotropic infiltration predominated by lymphocytes with a variable admixture of plasma cells and eosinophils.
Eczematous epithelial alterations.
A Warthin-Starry stain may be positive.
DDx: 1- Other causes of delayed hypersensitivity include other forms of arthropod assaults, drugs, and contactants 2- Connective tissue disease 3- Erythema annulare centrifugum.

20. Acrodermatitis CHRONICA ATROPHICANS
22-Treponemal Diseases
Lyme Disease
Acrodermatitis CHRONICA ATROPHICANS

21. Histopathology
Epidermis appears atrophic with loss of the rete ridges. There is granular layer diminution, and the epidermis is surmounted by a hyperkeratotic scale.
The papillary dermis appears edematous, with a grenz zone of collagen fibers oriented parallel to the epidermis.
A bandlike lymphocytic infiltrate is found in the mid and upper dermis.
The infiltrate is predominantly in an angiocentric, eccrinotropic, and folliculotropic disposition.

23. Borrelial lymphocytoma Cutis
22-Treponemal Diseases
Borrelial lymphocytoma Cutis

24. Histopathology
Superficial and deep angiocentric, neurotropic, and eccrinotropic lymphocytic infiltrates, often accompanied by plasma cells and eosinophils.
Epidermal spongiosis.
Spirochetes have been identified in the lesional border in only 40% of cases.
DDx: 1- Other forms of annular erythema 2- Other causes of lymphocytoma cutis 3- Malignant lymphoma 4- Other arthropod assaults 5- Drug hypersensitivity 6- Contact reactions 7- connective tissue diseases.