1. Elastica van Gieson-picro-fuchsin staining
The stable connective tissue staining for the differential analysis of nuclei, muscle and elastic fibres

2. Elastica van Gieson-picro-fuchsin staining
An overview staing using 3 different dyes Resorcin-Fuchsin: - elastic fibers Weigert‘s iron hematoxylin: - permanent nuclei stain Van-Gieson‘s picro-fuchsin: - connection tissue

3. Elastica van Gieson-picro-fuchsin staining
Fixaction recomandation: formalin or alcohol
infiltration, embedding, cutting, uptake, drying
Solvent
Time [min]
Process
xylol, ethanol series, det. water
deparaffinize, water
resorcin-fuchsin
15:00
elastic stain
tap water
01:00
rinse
Weigerst‘s iron hematoxylin
05:00
nuclear stain
10:00
wash out
hydrochloric acid alcohol 1 %
00:10
differentiate
dest. water
00:05
van-Gieson‘s picro-fuchsin solution
02:00
dye (1-5 min)
ethanol 96 %, isopropanol, xylol
water
xylol containing agent
cover

4. Elastica van Gieson-picro-fuchsin staining
Dyeing results on standard tissues: nuclei: brown-black connection tissue: red elastic fibres: black musculature: yellow cytoplasma: yellow neuroglia: yellow-orange mucus, amyloid, colloid, hyalin: yellow to red
Dyeing results on sponge tissues:
nuclei: purple-black
background tissue: pink
elastic fibres (spongin): purple
the stain reacts different on sponge tissue

5. Elastica van Gieson-picro-fuchsin staining
spongin
nucleus
cyanobacteria
spicules

6. Elastica van Gieson-picro-fuchsin staining

7. Elastica van Gieson-picro-fuchsin staining

8. Elastica van Gieson-picro-fuchsin staining