1. Accucore UHPLC & HPLC Columns
Mike Oliver Accucore Product manager
July, 2017

2. What is Accucore?

3. How Core Enhanced technology Delivers: Better Separations
Accucore UHPLC & HPLC columns are based on solid core particles which deliver fast and highly efficient separations.
Key Components

4. Why Core Enhanced technology works: Better Separations
The factors that affect chromatographic efficiency are resistance to mass transfer, longitudinal diffusion and eddy diffusion, the A, B and C terms respectively from the van Deemter equation.
Uniform packed bed
Reduced void volume
Fast mass transfer
Results in Better Separations

5. Accucore HPLC Columns – Features and Benefits
Ultimate Core Performance
Maximizing Your Investment
Fast Separations
Increase sample throughput and decrease solvent costs
High Peak Capacity
Separate and identify more peaks
Increased Sensitivity
Detect trace amounts
Lower Pressure
Use existing HPLC instruments or prolong the life of UHPLC instruments
Loading Capacity
Analyze a wide range of concentrations
Reproducible Chromatography
Have confidence in your separations
Long Lifetime
Use columns for longer
Wide Selectivity
Use the right column for your analysis

6. Accucore UHPLC & HPLC column family
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7. Accucore Vanquish UHPLC Columns

8. What is Accucore Vanquish?
Next generation UHPLC column
Founded on state-of-the-art Core Enhanced Technology
1.5 µm solid core particles
Designed in synergy with the high end UHPLC systems to deliver:
Better Separations: high efficiency enables the separation of very complex mixtures
More Results: High efficiency at high flow rates
Easier Interactions: Seamless workflow solution, for simple and easy separations

9. Core Enhanced Technology Delivers: Better Separations
More efficient at optimum flow than fully porous sub 2 micron
More efficient at optimum flow than 2.6 micron solid core
Wider “no-efficiency-loss” range than any other column technology
Effectively, as solution with Vanquish delivering unmatched resolution at high flows => pure productivity!

10. Why 1.5um provides: Better Separations
Efficiency = Better Separations
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1.25
1.50
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2.25
2.50
2.75
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3.25
3.50
3.75
4.00
4.25
4.50
4.75
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5.25
5.50
5.75
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6.25
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Accucore Vanquish 1.5 m, 295k N/m
Accucore 2.6 m, 196k N/m
Fully porous 1.7 m, 176k N/m
40% efficiency gain

11. Why Core Enhanced technology Delivers: More Results
Accucore Vanquish C µm 100 x 2.1mm
Solid Core C µm
100 x 2.1mm
The higher efficiency provided by the Accucore Vanquish column allows for shorter run times as we can increase the flow rate and at the same time provide better separations. In this example we can see we have a 40% time saving on the run time for the separation of 18 pesticides at a flow rate of 650 compared to 380ul/min compared to the Accucore 2.6um column.
Average increase in resolution of 24%
Average increase in peak capacity of 220%
40% time saving and Better Separations

12. Why Core Enhanced technology Delivers: More Results
Accucore Vanquish v’s 1.6µm competitor solid core column
Accucore Vanquish C µm 100 x 2.1mm
C µm
100 x 2.1mm
Average increase in resolution of 6%
Average increase in peak capacity of 11%

13. Why Core Enhanced technology Delivers: More Results
Accucore Vanquish v’s 1.7µm competitor solid core column
Accucore Vanquish C µm 100 x 2.1mm
C µm
100 x 2.1mm
Average increase in resolution of 17%
Average increase in peak capacity of 22%

14. Accucore Vanquish Delivers: More Results
Rapid Screening of 47 Drugs of Abuse in <4min
Overlaid selected-reaction monitoring chromatograms showing detection of 47 compounds

15. Accucore Vanquish Delivers: More Results
Separation of isobaric compounds
Chromatographic resolution of N-desmethyltapentadol and MDEA with out common precursor and product ions
Chromatographic resolution of codeine and hydrocodiene with common precursor (300 u) and product ions
Chromatographic resolution of naltrexone and 6-acetylcodine with common precursor (342 u) and product ions

16. Accucore Vanquish Delivers: More Results
Rapid Screening of 36 Veterinary Antibiotics in <5min
Overlaid selected-reaction monitoring chromatograms showing detection of 36 compounds

17. Why Core Enhanced technology Delivers: More Results
Reproducibility
The advanced bonding and automated packing technology used in the manufacture of Accucore Vanquish UHPLC columns results in exceptionally reproducible chromatography.
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18. Extended capabilities - Vanquish UHPLC columns
Next generation UHPLC columns delivering:
Better Separations: high efficiency enables separation of very complex mixtures
More Results: High efficiency at high flow rates
Easier Interactions: Seamless workflow solution, for simple and easy separations
Wider Capabilities: Wide range of column selectivity's and formats

19. Accucore UHPLC & HPLC Columns

20. Accucore HPLC Column Family

21. Accucore HPLC Column Family
Thermo Scientific™ Accucore™ HPLC columns provide HPLC and UHPLC users with increased sample throughput and improved assay robustness through fast, high resolution separations achieved at lower backpressures than those generated by columns packed with sub-2µm particles
Thermo Scientific™ Accucore™ columns for biomolecules provide nanoLC, HPLC and UHPLC users separating biomolecules with increased sample throughput and improved assay robustness through fast, high resolution separations achieved at lower backpressures than those generated by columns packed with sub- 2µm particles
Thermo Scientific™ Accucore™ XL HPLC columns allow users of conventional HPLC methods to enjoy increased peak resolution and lower limits of detection through much higher efficiencies than those offered by columns packed with 5 μm, 4 μm or even 3 μm fully porous particles

22. Accucore – Product Range
Formats
Analytical columns, Defender guard cartridges
Phases
Accucore RP-MS
Optimized for MS detection, excellent combination of speed and quality of separation
Accucore C18
Optimum retention for non-polar analytes
Accucore C8
Lower hydrophobicity than C18 recommended for analytes with moderate hydrophobicity
Accucore aQ
Compatible with 100% aqueous mobile phases, special selectivity for polar analytes
Accucore Polar Premium
Rugged amide embedded C18 phase that offers complementary selectivity to C18
Accucore Phenyl-Hexyl
Unique selectivity for aromatic and moderately polar analytes
Accucore Phenyl-X
Unique reversed-phase shape selectivity with high aromatic selectivity
Accucore PFP
Alternative selectivity to C18, particularly for halogenated analytes
Accucore C30
High shape selectivity for hydrophobic, long chain, structurally related isomers
Accucore HILIC
Enhanced Retention of polar and hydrophilic analytes
Accucore Urea-HILIC
Unique HILIC selectivity and low ion exchange activity

23. Accucore XL – Product Range
Formats
Analytical columns, Drop-in guard cartridges
Phases
Accucore XL C18
Compatible with conventional HPLC methods providing optimum retention for non-polar analytes
Accucore XL C8
Lower hydrophobicity than C18 recommended for analytes with moderate hydrophobicity and compatible with conventional HPLC methods

24. Accucore HPLC Columns High-pressure hardware 600 bar pressure rating
Etched end fittings
Purple Rings
4 lengths
30mm
50mm
100mm
150mm
3 IDs
2.1mm
3.0mm
4.6mm

25. Accucore Defender TM Guard Cartridges
Designed specifically to work with high speed, high efficiency separations
Compatible with UNIGUARD holders
Pack of 4 cartridges
1 length
10mm
3 IDs
2.1mm
3.0mm
4.6mm

26. Accucore – Speed Improve throughput and save costs
Minutes 1 2 3 4 5 6 7 8 9 10
-100
Accucore RP-MS 2.6m,100 x 2.1 mm
ΔP = 218 bar
Rs = 2.50
Fully porous C18 5m, 150 x 4.6 mm
ΔP = 59 bar
Rs = 2.64
Method Transfer Calculator:
Fully porous 5 μm 150 x 4.6 mm
35 – 60 %B in 10.0 min
17 min run time
1000 L/min solvent used 17 mL
Accucore RP-MS 2.6 μm, 100 x 2.1 mm
35 – 60 %B in 3.5 min
6 min run time
400 L/min solvent used 2.4 mL
Accucore RP-MS 2.6 μm, 50 x 2.1 mm
35 – 60 %B in 1.8 min
3 min run time
400 L/min solvent used 1.2 mL
Accucore RP-MS 2.6m, 50 x 2.1 mm
ΔP = 120 bar
Rs = 1.51
Improve throughput and save costs

27. Accucore - Same System, Same Method, Better Results
Column
P (bar)
N (plates)
Peak 2
S/N
FPP 5 µm
239
10538
534
XL 4 µm
312
18274
1202
Fully Porous C18, 5m
Columns: 150 x 4.6 mm
Mobile phase: 66.3:33.7 (v/v) water with phosphoric acid, pH 2.5:methanol
Flow rate: 2 mL/min
Column temperature: 30 °C
Detection: UV at 214 nm
Injection volume: 5 µL
Valerophenone
Ibuprofen
Accucore XL C18, 4 m
73% higher efficiency
125% higher sensitivity

28. Accucore – Ruggedness and Reliability
Accucore XL C8 4.0 μm 50 x 2.1 mm
Injection 2200
Injection 1500
Injection 750
Injection 1
Separation of naproxen, fenoprofen and ibuprofen, with theophylline as a t0 marker
Rely on columns and get extended use from them

29. Accucore BioLC Columns

30. Accucore HPLC Column Family

31. Accucore HPLC Column Family
Thermo Scientific™ Accucore™ HPLC columns provide HPLC and UHPLC users with increased sample throughput and improved assay robustness through fast, high resolution separations achieved at lower backpressures than those generated by columns packed with sub-2µm particles
Thermo Scientific™ Accucore™ columns for biomolecules provide nanoLC, HPLC and UHPLC users separating biomolecules with increased sample throughput and improved assay robustness through fast, high resolution separations achieved at lower backpressures than those generated by columns packed with sub- 2µm particles
Thermo Scientific™ Accucore™ XL HPLC columns allow users of conventional HPLC methods to enjoy increased peak resolution and lower limits of detection through much higher efficiencies than those offered by columns packed with 5 μm, 4 μm or even 3 μm fully porous particles

32. Accucore Bio – Product Range
Formats
Analytical columns, Defender guard cartridges, nanoViper columns
Phases
Accucore 150-C18
Phase designed for the separation of peptides
Accucore 150-C4
Lower hydrophobicity for optimal retention of proteins and larger peptides
Accucore 150-Amide-HILIC
Designed for the separation of hydrophilic biomolecules in HILIC mode. An excellent choice for glycan separations

33. Separate peaks of interest
Accucore – Resolution
Accucore 150-C18
Peak no.
Peptide 1 GY 2
VYV 3
Met-Enkephalin 4
Angiotensin III 5
Leu-Enkephalin 6
Ribonuclease A 7
Insulin
Conventional Solid Core C18
< 2 µm Wide Pore Fully Porous C18
Separate peaks of interest

34. Proteins – Excellent Resolution vs < 2 µm Wide Pore
Accucore 150-C4
Backpressure: 185 bar
Sharper and higher peaks than < 2 µm Wide Pore Fully Porous C4
Better resolution and sensitivity
Significantly lower backpressure
< 2 µm Wide Pore Fully Porous C4
Backpressure: 320 bar

35. Peptides – High Peak Capacity – greater sensitivity
Higher peak capacity than
Conventional solid core C18
< 2 µm wide pore fully porous C18
Higher peak capacities facilitate increased peptide identifications

36. Peptides – Reproducible Separations
Peak #
Name tR
% RSD 7
Insulin
13.58
0.11
Precision of retention times is critical for reliable analysis
The Accucore 150-C18 column exhibits excellent retention time reproducibility

37. Proteins – Reproducible ResultstR
% RSD
PWHH As
Ovalbumin (45 kDa)
9.30
0.06
0.05
1.14
Precision of retention times is critical for reliable analysis
The Accucore 150-C4 column exhibits excellent retention time reproducibility

38. Proteins – Proven Loading Capacity
As the load on the Accucore 150-C4 column increases up to 1200 pmoles peak shapes remain very sharp

39. Positioning

40. Market Requirements Omics Bioanalysis Clinical Environmental
Complexity of sample
Bioanalysis
Clinical
Environmental
HTS/Library
QA/QC
Speed of Analysis

41. Product Positioning Accucore BioLC Accucore Vanquish UHPLC
Complexity of sample
Accucore 2.6µm
UHPLC/HPLC
Accucore 4µm
HPLC
Speed of Analysis

42. Supporting collateral?

43. Marketing Plans www.thermofisher.com/accucore
Technical Guide
Phase guide
Application notes
Webinars
Commercial web based training
One minute highlight
Videos
Social media
Landing page
Product page
Method conversion tool
Advertorials
www. appslab.thermofisher.com

44. Accucore Column Family Summary
Accucore UHPLC & HPLC Columns
Accucore Columns for Biomolecules
Accucore XL HPLC Columns
Ultimate Core Performance - Speed and Selectivity Combined
Solid core particles for fast separations with superb resolution
Wide range of phases to meet all your separation needs
Exceptionally rugged and easy to use columns