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Absence of association between IL-28B gene polymorphism (rs SNP) and sustained virological response in Iranian patients with chronic Hepatitis C
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Introduction Epidemiology Chronic Hepatitis C
Ref:Hepatitis C VirusesGenomes and Molecular Biology PhD. Lilly Research Laboratories, Indianapolis, USA Norfolk (UK): Horizon Bioscience; 2006.ISBN-10: ISBN-13:
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Introduction Risk Factors for Developing Chronic HCV Infection
Ref: Stephen L. Chen, International Journal of Medical Sciences,2006
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Introduction Treatment: The cure rate depends on several factors:
HCV genotype, past treatment experience, eligibility to take the drug interferon. currently recommended regimen: Is based on the use of pegylated interferon (PEG-IFN) combined with ribavirin (RBV) for 24 to 72.
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Introduction Interleukin 28B (interferon-λ 3):
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The SNP is located on chromosome 19q13
Rs The SNP is located on chromosome 19q13 It is 3 kilobases downstream of the interleukin-28B SNP genotypes: TT TG GG In many studies it has been proved that “G” allele is strongly associated with NVR (none virological response). Ref:Prediction of response to pegylated-interferon-α and ribavirin therapy in Chinese patients infected with different hepatitis C virus genotype, Xiaoyan Guo ,China,2009
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Materials & Methods Our research aimed at:
Identifying rs SNP polymorphism assotiation with the response to Hepatitis C treatment among infected Iranians.
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Materials & Methods 71 +SVR 22 -SVR 57 Healthy individua
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Materials & Methods Statistic Analysis PCR RFLP BsemI DNA Extraction
Electrophoresis
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Electrophoresis of PCR products:
Results Electrophoresis of PCR products: 400bp Negative control SVR(+) SVR(-) 1 2 3 4 5
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Electrophoresis of digested PCR products:
Results Electrophoresis of digested PCR products: M 1 2 3 4 5 6 400 234 166
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Genotype frequency of case and control groups
Results Genotype frequency of case and control groups Host genotype Control(n(%)) Case(n(%)) P-value OR** 95% CI*** TT* 43(75) 53(57) 0.06 TG 13(23) 33(35) 2.06 GG 1(2) 7(8) 0.11 5.67 T* 99 139 G 15 47 0.013 2.23
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Genotype frequency of case and control groups
Results Genotype frequency of case and control groups
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Results: Genotype frequency among case group
Host genotype SVR+ N (%) SVR- Pvalue Or** 95%ci*** TT* 46(65) 7(32) 0.01 TG 19(27) 14(64) 0.003 0.20 GG 6(8) 1(4) 0.93 0.91 T* 111 28 G 31 16 0.055 .489
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Genotype frequency among positive SVR and non-SVR
Results Genotype frequency among positive SVR and non-SVR
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They showed that 91% of GG allele carriers did not achieve SVR.
Discussion Spain , 2010 Positive SVR: TT:83.5% TG:10.5% GG:6% They showed that 91% of GG allele carriers did not achieve SVR.
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Discussion Germany, 2010 Positive SVR: TT:80.5% GG:87.5%
They showed no correlation between rs polymorphism and SVR. In our study we concluded the rate of SVR as : TT:86% GG: 85%
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Discussion Brazil, 2013 Frequency of T allele among Hepatitis C patients was 63.3% whereas 75.5% among Healthy people. G allele was 1.7 time higher between infected people. They did not find any correlation between the treatment and the polymorphism neither did we.
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Conclusion Totally 150 people including 93 HCV infected patients and 57 healthy individuals entered the study. G allele can increase the risk of Hepatitis C disease 2.23 time. We did not find significant difference between case group and control group in term of rs genotypes distribution. There is no correlation between rs polymorphism and SVR.
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Future prospective Studying more samples in Iranian population.
Considering more HCV genotypes if possible. Conducting the same research on other related genes such as TGFβ and TNFα.
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Acknowledgment Dr.Seyed Mehdi sadat, Molecular Genetics assistant professor Dr.Mehri Nikbin, Infectiuos diseases specialist Hepatitis & Aids Department of Pastuer Institiue of Iran
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Thank you for your attention!
It Strongly Can Overcome Medical Struggles iscoms Thank you for your attention!
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