1. GREEN BOOK REVIEWS – STATE LAB
DIFFUSION
PAGES

2. Diffusion through a Membrane
A model cell setup is represented in the
“Initial State” diagram to the right.
Which diagram indicates the areas where each of these substances would be located after 20 minutes?
Part of a laboratory procedure is shown in the diagram to the right.
This setup would most likely be involved in a procedure to
(1) stain specimens while making a wet mount
(2) test for the presence of glucose using an indicator
(3) separate pigments in a mixture
(4) determine the pH of solutions

3. A student prepared four different red blood cell suspensions, as
shown in the chart to the right.
Which suspension would contain red blood cells that would appear wrinkled and
reduced in volume?
(1) A (3) C
(2) B (4) D
The change in red blood cell volume is principally due to the movement of
(1) serum (3) water
(2) oxygen (4) salt
Which process is most likely involved in the change in
red blood cell volume?
(1) active transport (3) replication
(2) evaporation (4) diffusion
The diagram to the right shows a student heating some test tubes with chemicals in them during a laboratory activity. Explain why putting stoppers in the test tubes could be dangerous.
*Test tubes could explode

4. Identify the color of the contents of the artificial cell after two hours.
*blue-black
After two hours, the color of the liquid in the beaker did not change. This shows that
(1) glucose moved from the artificial cell into the beaker
(2) starch did not pass out of the artificial cell
(3) starch was digested to glucose in the artificial cell
(4) glucose molecules combined to produce starch in the artificial cell
This laboratory setup would most likely be used to demonstrate
(1) carbohydrate synthesis
(2) active transport
(3) diffusion
(4) dehydration
A laboratory setup using an artificial cell made from dialysis tubing is shown in the diagram to the right.
Identify the process that would most likely be responsible for the movement of glucose
from inside the artificial cell to the solution outside of the cell.
*passive transport

5. A wet-mount slide of red onion cells is studied using a compound light microscope. A drawing of one of the cells as seen under high power is shown to the right.
On the diagram below, label the location of each of the cell structures listed.
cell wall
cytoplasm
cell membrane
*cytoplasm
*cell wall
*cell membrane
In the space below, sketch how the cell would look after the saltwater solution is added
to it.
Describe the proper way to add a saltwater solution to the cells without removing the
coverslip.
*drop of salt water on side of coverslip & paper towel on other side.

6. A student observes some cells with a compound light microscope as shown in view A to the right.
What did the student most likely do to obtain view B?
(1) applied a biological stain to the slide
(2) applied distilled water to the slide
(3) used electrophoresis
(4) used a higher magnification
A laboratory technique is illustrated in the
diagram to the right.
The technique of lowering the coverslip at an angle is used to
(1) make organelles more visible
(2) reduce the formation of air bubbles
(3) make the specimen transparent
(4) reduce the size of the specimen
An experimental setup using a model cell is shown in
the diagram to the right.
State what cell structure the dialysis tubing represents.
*a cell

7. Information about which two lettered parts is needed in order to determine the total magnification of an object viewed with the microscope in the position shown?
A-ocular lens C-objective lens
____________ and ____________
Which lettered part should be used to focus the image while using high power?
E-fine adjustment
State two ways the image seen through the microscope differs from the actual specimen being observed.
upside down & backwards
Two molecules, A and B, and their distribution inside and outside of a cell are represented in the diagram to the right.
State one possible reason why molecule A could diffuse across the membrane of the cell but molecule B could not.
Molecule A = small & molecule B = too big

8. Starch turns blue black in the presence of a starch indicator
Starch turns blue black in the presence of a starch indicator. Dialysis tubing tied at both ends and containing starch solution is placed in a beaker of water. Yellowish brown starch indicator is then added to the water.
What will the solutions in the beaker and the tubing look like after 20 minutes?
(1) The indicator solution in the beaker will be blue black and the starch solution in the
tubing will not change color.
(2) The starch solution in the tubing will be blue black and the indicator solution in the
beaker will not change color.
(3) Neither the indicator solution nor the starch solution will be blue black.
(4) Both the indicator solution and the starch solution will be blue black.
This laboratory setup would most likely be used to demonstrate the process of
(1) diffusion (3) replication
(2) active transport (4) cellular respiration
Describe what will happen to red onion cells in a wet-mount slide when a saltwater
solution is added to them.
*water moves out & cell shrinks.
Some of the pain from a sore throat is caused by swelling of moist throat tissue. A common remedy for a sore throat is to gargle (rinse the throat tissue) with salt water. Explain why gargling with salt water would be expected to relieve the pain of a sore throat.
*water moves out of cell (diffusion) = decrease swelling

9. Diffusion through a Membrane
A wet mount of red onion cells as seen with a compound light microscope is shown to the right.
Which diagram best illustrates the technique that would most likely be used to add salt to these cells?
In the space below, sketch what cell A would look like after the addition of the salt.
Which substance would most likely be used to return the cells to their original condition?
(1) starch indicator
(2) dialysis tubing
(3) glucose indicator solution
(4) distilled water