1. Catalyst What are the subunits of proteins?
What organelle makes proteins?
What type of bonds hold amino acids together in proteins?

3. Read + pre-lab questions

4. Pre-lab questions

5. PRIMARY
SECONDARY v
TERTIARY
QUATERNARY

6. protein

7. How do you think the message gets from the nucleus to the cytoplasm!?
Think, Pair, Share
Genes that are made of DNA are the instructions for making proteins. DNA is found in the nucleus.
BUT! Proteins are made by ribosomes in the cytoplasm.
How do you think the message gets from the nucleus to the cytoplasm!?

8. 2 steps in protein synthesis
Make an mRNA strand with the DNA message
Nucleus

9. SINGLE
DOUBLE
RIBOSE
A, U, G, C
A, T, G, C

10. U A G G C U C A A

11. 10 min.

12. 2 steps in protein synthesis
Ribosome reads the mRNA and makes a protein
Cytoplasm - ribosome

13. Translation animation!

14. Translation Animation (again!)

15. Remember: codons = 3 bases
Example:
AGU=
GCC=

16. Codon Practice 5 min. with partner
U4-13
1. Name the amino acids that are coded by the following codons:
a. AAA e. CAC
b. UAC f. UGA
c. GGG g. AGC
d. GGA h. CCC
2. Write the amino acid sequence to make up the protein:
GCA - GGU - CCA - AUG – UGC
3. Write the amino acid sequence to make up the protein:
GCA - GGU - CCG - AUA - UGC

17. Example: - CCC = __________ - CCU = __________ - CCG = __________1 1
neutral
See for yourself!
Example:
- CCC = __________
- CCU = __________
- CCG = __________
- CCA = __________
Original DNA:
G G G = ______
Mutated DNA:
G G A = ______

18. What is this stop codon?!

19. What is this start/initiator codon?!
Always AUG

21. 10 min

22. Shout outs must be written in the first 5 minutes…submit them!!
Catalyst
Shout outs must be written in the first 5 minutes…submit them!!
This is the primary structure of a protein.
What do the yellow circles represent?
What do the blue lines represent?
Draw an example of a folded protein.
This molecule is made inside a cell. Which organelle “synthesizes” it?

23. protein

24. How do you think the message gets from the nucleus to the cytoplasm!?
Think, Pair, Share
Genes that are made of DNA are the instructions for making proteins. DNA is found in the nucleus.
BUT! Proteins are made by ribosomes in the cytoplasm.
How do you think the message gets from the nucleus to the cytoplasm!?

25. 2 steps in protein synthesis
Make an mRNA strand with the DNA message
Nucleus

26. SINGLE
DOUBLE
RIBOSE
A, U, G, C
A, T, G, C

27. U A G G C U C A A

28. 10 min.

29. mRNA
codon
codon
codon

31. 2 steps in protein synthesis
Ribosome reads the mRNA and makes a protein
Cytoplasm - ribosome

32. Translation animation!

33. Translation Animation (again!)

34. Remember: codons = 3 bases
Example:
AGU=
GCC=

36. Codon Practice 5 min. with partner
U5-5
1. Name the amino acids that are coded by the following codons:
a. AAA e. CAC
b. UAC f. UGA
c. GGG g. AGC
d. GGA h. CCC
2. Write the amino acid sequence to make up the protein:
GCA - GGU - CCA - AUG – UGC
3. Write the amino acid sequence to make up the protein:
GCA - GGU - CCG - AUA - UGC

37. Example: - CCC = __________ - CCU = __________ - CCG = __________1 1
neutral
See for yourself!
Example:
- CCC = __________
- CCU = __________
- CCG = __________
- CCA = __________
Original mRNA:
G G G = ______
Mutated mRNA:
G G A = ______

38. What is this stop codon?!

39. What is this start/initiator codon?!
Always AUG

41. 10 min

43. Exit ticket IN YOUR OWN WORDS explain how protein synthesis occurs in a cell. Underline the following words in your response:
Nucleus
Polypeptide/protein
Ribosome
Transcription
DNA/gene
Translation
Amino acid
Codon
mRNA
Anticodon
tRNA

44. This is the gene (DNA) that makes hemoglobin, a protein in your blood.
Catalyst
This is the gene (DNA) that makes hemoglobin, a protein in your blood.
A T G T A C C G A A A T
Transcribe the gene into mRNA:
Translate the mRNA into protein:

45. U5-8

46. U5-9

47. Insertion deletion substitution
DNA sequence
negative
Insertion deletion substitution
Pg U5-10

48. Tay-Sach’s Disease

49. deleted
inserted
AAA CCT TTG GG
AAA CCC ATT TGG G
ACA CCC TTT GGG

51. CFU What is a mutation? What are the 3 types of mutations?
CGA TTC GGA TGA
The above DNA strand undergoes a DELETION mutation at the END. Write the mutated strand.

53. Sickle cell anemia

55. Protein Synthesis Practice…again!
Pg U5-12 & U5-13 Staple these into your unit 5 packets!

56. MQ1 5 minutes to review your notes & practice!
Protein Synthesis
MQ1 5 minutes to review your notes & practice!

57. Picture word bank mRNA Transcription Translation tRNA Amino acid
Protein/polypeptide
Ribosome

59. catalyst
If you could clone (make a copy) of any LIVING thing – what would you clone? Why?
If you could pick your baby’s characteristics (physical traits)
would you?

61. ON OFF environment expressed needs genes environment
U5-11.5 ON
OFF
environment
expressed
needs
genes
environment
traits

62. Biotechnology
5 “stations”
4 minutes each
Fill in your notes!

64. During the videos, fill out the corresponding reflection questions for each type of biotechnology

65. Biotechnology

66. Human Genome Project

67. Gene Therapy

68. Stem Cell Research

69. Cloning

70. Work Time
Complete ALL unfinished practice in your packet. U5-6 through U5-13 should be completed by the end of today. Work together. DO NOT copy. Copying = plagiarism = 0

71. catalyst a. b. c. Where does transcription happen in the cell?
Where does translation happen in the cell?
What are a, b, and c in the labeled picture? a. b. c.

72. catalyst a. b. c. Where does transcription happen in the cell?
Where does translation happen in the cell?
What are a, b, and c in the labeled picture? a. b. c.

73. catalyst Turn in your U5 Vocab! (or a pink slip!) What is molecule 1?
What process is occurring at arrow #1?  
What process is occurring at arrow #2? #1 #2

74. genetic
DNA
DNA
one
insulin
bacterial

76. fragments
size
compare
relationships

77. 10 minutes

78. Biotechnology Review your notes for 5 minutes…
U5 MQ 2
Biotechnology Review your notes for 5 minutes…

79. Catalyst
Copy the following onto your catalyst sheet and then match: Column 1 Column 2 Transcription Replication Translation
DNA  DNA
DNA  mRNA
mRNA  protein

80. Unit 5 Review Choose 3: 15 minutes @ each station
Fact first: complete the review of protein synthesis
U5 Crossword: complete the vocabulary review!
Protein Synthesis Online Lab: *computer*
Gel Electrophoresis: solve the cases!
Know your pictures!: complete the labeling/identification of structures
Mr. Lascano: Complete study guide, quiz corrections, and organize your notebooks
15 each station

82. #tbt
If a bear’s brain cell has 20 chromosomes, how many chromosomes are in his sperm?
Which image below shows mitosis?
Which image below shows meiosis?

83. Unit 5 Test DO NOT WRITE ON THE TEST When you finish:
CHECK your answers
Turn it in at the front
Remain seated and silent