1. An introduction to the Compound Light Microscope (This is a note check, for p. 3) You must make sure you have these correct for studying them later.

2. Answer to question 1 Eyepiece lens
Simple microscopes use one lens to magnify.
Compound microscopes use the eyepiece and objective lens (2 lenses) to magnify.

3. MICROSCOPE USE-SAFETY
Carry the microscope with both hands, one under the base the other holding the arm and keep microscope close to your body.
Answer to question 2

4. MICROSCOPE USE-SAFETY
Fine adjustment is used for focusing on high and medium power.
Answer to question 3

5. MICROSCOPE USE-SAFETY
Coarse adjustment is used to raise and lower the stage
Coarse adjustment is used to focus on low power
Answer to question 4

6. MICROSCOPE USE-SAFETY
When raising and lower the stage (all the way up or all the way down your eyes are looking from the side.
You do this so the lenses don’t hit the stage.
Answer to question 5

7. MICROSCOPE USE-SAFETY
To change magnification you turn the nosepiece. You watch from the side. because you don’t want to hit the lens against the stage.
Answer to question 6

8. Putting the microscope away
Answer to question 7
a. Lower the stage
b. Change to low power
c. Wrap the cord
d. Put the dust cover on
e. Slide it onto the correct shelf with the arm facing you

9. Image is reversed by the lenses bending light during magnification
Answer to question 8

10. You need thin specimens with this microscope
Why? Light needs to pass through and enter lenses to magnify image.
Answer to question 9

11. 1st focus is how you set up the microscope when you first look
1. When you know the routine, it’s easy.
2. It really works!
Answer to question 10

12. FIRST FOCUS TECHNIQUE 1. Lower the stage 2. Put on low power
3. Put on low light 4. Put object into
center of aperture
5.Raise stage 6. Look and see
* A SMALL AMOUNT OF FOCUSING WILL BE NEEDED BUT IT WILL THERE
Aperture - the small “hole” in the middle of the stage.
Answer to question 11

13. *Labels go outside the circle, (field of view).
1 . Your first SKETCH
*Label 3 pixels, (3 different colors) on medium power.
*Labels go outside the circle, (field of view).
*Use a ruler for straight label lines.
*Draw and color the whole field of view.
Name=_________________
Location=_______________
Power = _______________

14. Answers questions to guide your learning
Diagram
Answers questions to guide your learning

15. Rotates =prism housing
# 1
# 2

16. Eyepiece Also called ocular 10 X Built in pointer
Turning eyepiece - moves pointer

17. Body tube
Not strong
To move it turn the prism housing
Prism
housing

18. # 10
# 14
# 13
#15

19. Lamp Switch
If the lamp does not come on check the switch.

20. Base Don’t tilt microscope off base while looking.
break body tube/prism housing

21. Coarse Adjustment knob
Is used to raise and lower the stage of the microscope when first setting up or when putting the microscope away.
It is used for focusing on low power.
It does not get used with medium and high power

22. Fine Adjustment It is used on medium & high power
Turning it more than once or twice means you should go back to low power & do 1st focus again
Over using it will result in it not working.

23. Resetting FINE ADJUSTMENT
Indicator marks are part 16 in the diagram
When fine adjustment is not working look at the marks and turn fine adjustment until the one mark is moved to the middle of the two other marks
This is how you reset fine adjustment

24. #12 #9

25. Stage
Holds slide

26. # 11

27. Diaphragm It is a disc with five openings.
By lining up a big or small hole more or less light enters the lenses.changes light
Adjusting the amount of light is done with the diaphragm

28. # 4
# 8

29. Nosepiece You turn the nosepiece to switch lenses (objectives)
It has a click that you feel when it is locked into position.
You look from the side when doing this because do not want to hit the lens against the stage

30. Aperture The opening in the stage
It has a non-magnifying “lens” and should be kept clean and dry
The object you wish to look at should be centered in the aperture

31. # 3
# 7
# # 8

32. Arm
Faces you

33. Stage Clips
Replaced with a mechanical stage

34. Correct way to put slide in
Open the arm
Place the slide all the
way back so it is snug
in the corner
Close arm slowly
Turn the knobs to move specimen to the center of the aperture

35. # 5 #6

36. Total Magnification
The power of magnification is marked on each objective.
Low power is a 4X, medium is a 10X and high is a 40X lens
Because the eyepiece is a 10X lens when using low the total magnification is 10X times 4X or 40X. Low power is the best lens to search or scan with, it sees a large area.
The TM of medium is 100X because the eyepiece is 10X and medium is 10X.
The TM of high is 400X magnification it is the worst lens to try to find something

37. TM Low Power Scanning – Searching – sees large area
Low Power Objective (4X) Eyepiece Lens (10X)

38. What it looks like

39. TM Medium Power

40. What it looks like

41. TM High Power

42. What it looks like

43. 1st focus or Initial focus
Focusing
Adjust the distance the stage is from the lens
1st focus or Initial focus
Watch from side
raise stage coarse adj.
Look in the eyepiece focus down with coarse adj.

44. Dry Mount

45. Seeing “thickness” High power Move fine up & down
Layers come in & out of focus.
Called “DEPTH OF FIELD”

46. Wet Mount Because it uses water, is called a wet mount or wm
Drop of water and specimen
Cover glass
Because it uses water, is called a wet mount or wm

47. 3 reasons Wet Mounts are made
Increases light passing through
Flattens object
3. Keeps living material moist

48. (slide and cover glass). Blot dry
Cleaning slide & cover
Rinse both
(slide and cover glass).
Blot dry

49. End of period: slide & cover glass clean up
Wipe off moisture
DON’T WASH!!!!!!
Why?
You’ll go late to next class. Takes too long

50. Checkout Dry slide, cover glass & return to tray Scope
low power, lower stage, # shelf, wrap cord, dust cover

51. Adding water to wet mount slide

52. Your drop adding tool Finger = add “1” drop
Moisten finger at the sink.
“1” drop added to slide

53. Low cover glass slowly - avoid air pockets

54. Place the object on the slide

55. Gently lower c.g.

56. Absorbed extra water with p.t.
Paper towel

57. Drawing it
Water
Cover glass
Object or specimen

58. No water Dry Mount Slide Object, slide, cover glass
Used for inanimate objects or
things that do not need water to live.

59. DM hair strand of hair (trim it to fit under c.g.)
cover glass over the hair

60. Simple Stain added to WM
1 drop of stain on edge of the cover glass
paper towel on the other edge
Draw water and draw the stain under the cover glass.

61. P.T. draws stain under cover glass
Paper towel
stain

62. Extra stain
Blot the excess stain with a paper towel
Why?