1. Using a Microscope: Veterinary Medical Applications 12c

2. What is this?
Cross-section of human skin

3. What is this?
White blood cells attacking pathogen in the blood stream

4. What is this?
Butterfly’s wing

5. What is this?
pantyhose

6. What is this?
Shark skin

7. Today You Will Be Able To…
Identify the parts of a microscope
List common safety and handling procedures for using microscopes
Demonstrate the ability to properly focus, change magnification, and adjust field of vision in a microscope

8. Identify the Parts of the Microscope
Objective 1
Identify the Parts of the Microscope

9. Identify the parts

10. Arm
The basic frame, used to CARRY the microscope.

11. ARM

12. BASE
The bottom stand, which houses the LIGHT.

13. ARM
BASE

14. Body
Contains MIRRORS to reflect light to the eyepiece.

15. BODY
ARM
BASE

16. COARSE Adjustment
Roughly focuses the image by bringing the specimen and objective lenses closer together or farther apart.

17. BODY
ARM
COARSE ADJUSTMENT
BASE

18. Diaphragm
A disc-shaped device, located beneath the stage, used to control the AMOUNT of LIGHT passing through the specimen.

19. BODY
ARM
COARSE ADJUSTMENT
DIAPHRAGM
BASE

20. Eyepiece
The UPPER lens, nearest your eye, which magnifies the image already magnified by the objective and brings the light rays to a focus at the eye.

21. EYEPIECE
BODY
ARM
COARSE ADJUSTMENT
DIAPHRAGM
BASE

22. FINE ADJUSTMENT
SHARPENS the image of the specimen, by moving the specimen and objective lenses very small distances closer together or farther apart.

23. EYEPIECE
BODY
ARM
COARSE ADJUSTMENT
DIAPHRAGM
FINE ADJUSTMENT
BASE

24. Light
The SOURCE of the light which passes through the specimen.

25. BODY ARM BASE EYEPIECE COARSE ADJUSTMENT DIAPHRAGM FINE ADJUSTMENT
LIGHT
BASE

26. Nosepiece
The REVOLVING part that supports three objectives with different magnifications.

27. BODY ARM BASE EYEPIECE NOSEPIECE COARSE ADJUSTMENT DIAPHRAGM
FINE ADJUSTMENT
LIGHT
BASE

28. Objective
The lenses closest to the specimen, that MAGNIFIES the image of the specimen.

29. BODY ARM BASE EYEPIECE NOSEPIECE OBJECTIVE COARSE ADJUSTMENT DIAPHRAGM
FINE ADJUSTMENT
LIGHT
BASE

30. STAGE
The flat table-like AREA where the slide or specimen is placed.

31. BODY ARM BASE EYEPIECE NOSEPIECE OBJECTIVE STAGE COARSE ADJUSTMENT
DIAPHRAGM
FINE ADJUSTMENT
LIGHT
BASE

32. Stage Clips
HOLDS the slide on the stage, to prevent it from slipping around.

33. BODY ARM BASE EYEPIECE NOSEPIECE OBJECTIVE STAGE STAGE CLIPS
COARSE ADJUSTMENT
DIAPHRAGM
FINE ADJUSTMENT
LIGHT
BASE

34. BODY ARM BASE EYEPIECE NOSEPIECE OBJECTIVE STAGE STAGE CLIPS
COARSE ADJUSTMENT
DIAPHRAGM
FINE ADJUSTMENT
LIGHT
BASE

35. Can You Remember Them All???

37. List and define common microscope terms
Objective 2
List and define common microscope terms

38. Slide
A piece of GLASS upon which the specimen is placed for viewing.

39. Cover Slip
A thin SQUARE piece of glass or plastic used to COVER the specimen.

40. High power
The high power objective on the nosepiece (usually 40x).

41. Medium power
The middle power objective on the nosepiece (usually 10x).

42. Low power
The lowest power objective on the nosepiece (usually 4x).

43. Magnification
The number of TIMES an objects apparent size is INCREASED by the lens system.
The total magnification of the lens system is determined by MULTIPLYING the power of the objective times the power of the eyepiece (usually 10x).

44. Resolution
The ability of the lens system to distinguish and SEPARATE fine details in a specimen.

45. Field of view
The AREA of the specimen which is seen through the lens system. The bright circle you see when looking into a microscope.

46. Depth of field
The ability of a lens system to furnish a distinct image ABOVE and BELOW the focal plane. Depth of focus decreases with the increase of magnification.

47. Parfocal
When the objective is in focus, it can be SWITCHED from one power to another, with only small fine focus adjustments being necessary.

48. List common safety and handling procedures for using microscopes
Objective 3
List common safety and handling procedures for using microscopes

49. Use of the Microscope 1. Carry with BOTH hands.
2. Set the microscope in from the EDGE of the desk.

50. Use of the Microscope
3. Use lens paper to CLEAN eyepiece and objectives.
4. START with the LOW power (shortest objective) in place.

51. Use of the Microscope
5. Turn the coarse adjustment so that the STAGE is as close to the low power as possible -- now while looking, turn the coarse adjustment so the stage starts to move away from the objective.
6. CLEAN up all spills and moisture from stage before putting away.

52. Use of the Microscope
7. Caution -- keep all WATER away from electrical parts
8. Never use the COARSE adjustment with the HIGH power objective while trying to focus, because of the danger of striking and breaking the glass slide with the objective.

53. Objective 4
Demonstrate the ability to properly focus, change magnification, and adjust field of vision in a microscope

54. Demonstrate your understanding of microscope use
Refer to the lab worksheet