1. Good Afternoon! FIND YOUR NAME ON THE POST-IT NOTES Warm Up- Today:
Grab the two sheets on the front desk.
Begin answering the questions using the lab cartoon.
Pass forward your homework. (book WS)
Today:
Microscope notes
Finish lab
View microscope slides
Class work

2. Good Afternoon Warm Up: Today:
Microscope labeling on front desk…Do not use your notes!
Today:
Characteristics of Life notes
Class work
Microscope Quiz

3. The Compound Light Microscope

4. What is a compound light microscope?
Mechanism for enlarging
an image

5. Magnification The two lenses are called:
Eyepiece
Objective
The eyepiece has a magnification of “10x”
The magnification of the objective lenses vary and are marked on the lens
Total magnification =
(Eyepiece) X (Objective)
Example:
10 X 40 =
400

6. What is the Total Magnification?
What’s my power?
What is the Total Magnification?
400x

7. The Parts of the Microscope and Their Function
Maintains
proper distance
between lenses
Magnification
Rotates the objectives
Support body tube
Magnification
Low, medium,high(greatest magnification)
Supports slide
Holds slide
In place
Moves stage up-
down/Focuses image
Regulates
amount of light
Sharpens the image
Reflects light
towards eyepiece
Supports microscope

8. Proper Procedures
Carry the microscope with one hand under the base while grasping the arm with the other hand
Be careful of the cord when moving around the lab tables!!

9. Proper Procedures
2. Rotate the nosepiece to obtain the proper objective lens
Make sure the lens clicks into place!!
3. Look through the eyepiece and switch on the light
This is your field of view!

10. Do not use course adjustment with high power!
Proper Procedures
4. Place slide on stage, center specimen over stage
5. Locate specimen on scanning or low power using coarse adjustment
6. Carefully switch to high power
7. Focus image with fine adjustment ONLY on high power!!!!!!!
Avoid damaging slide!
Do not use course adjustment with high power!

11. Storing the Microscope
Four steps prepare the microscope for storage:
the 10X objective is in place
the stage is all the way down
the power is off
the cord is wrapped around the base

12. You do not need to use the stage clips when viewing wet-mount slides!
How to make a wet-mount slide …
1 – Get a clean slide and coverslip from your teacher.
2 – Place ONE drop of water in the middle of the slide. Don’t use too much or the water will run off the edge and make a mess!
3 – Place the edge of the cover slip on one side of the water drop.
4 - Lower the cover slip on top of the drop to prevent air bubbles
Cover Slip
Lower slowly
You do not need to use the stage clips when viewing wet-mount slides!
5 – Place the slide on the stage and view it first with the lowest power objective. Once you see the image, you can rotate the nosepiece to view the slide with the different objectives.

13. Homework QUIZ Friday! Characteristics packet due Tuesday
Microscope notes
Characteristics packet due Tuesday

14. GOOD MORNING!!! I’ll collect your homework. No Warm Up today Today:
Notes
Class work
QUIZ TOMORROW

16. Let’s Review! Eyepiece Body tube Nosepiece Arm Low Medium Stage9.
10.
11.
14.
Eyepiece 1. 2. 3. 4. 5. 6. 7. 8.
Body tube
Nosepiece
Arm
Low
Medium
Stage
High power
Stage clips
12.
Course adjustment
Diaphragm
13.
Fine adjustment
Light source
Base

17. So Let’s Review Let’s Review! Eyepiece Body tube Nosepiece Arm
Scanning
Low Power
Stage
High power
Stage clips
Course adjustment
Diaphragm
Fine adjustment
Light source
Base

19. Comparing Powers of Magnification
We can see better details with higher the powers of magnification, but we cannot see as much of the image.
Which of these images would be viewed at a higher power of magnification?

20. References http://www.cerebromente.org.br/n17/history/neurons1_i.htm
Google Images

21. Evolution of the microscope
Choice of lens
magnification
2 set lenses

22. Anthony van Leeuwenhoek
The History
Hans and Zacharias Janssen of Holland in the 1590’s created the “first” compound microscope
Anthony van Leeuwenhoek and Robert Hooke made improvements by working on the lenses
Robert Hooke
Anthony van Leeuwenhoek
Hooke Microscope

23. The History
Zacharias Jansen
The “First” Microscope