1. Microscope
Basics
T. Tripe

2. The History
Zacharias Jansen
The “First” Microscope

3. The History Many people experimented with making microscopes
Was the microscope originally made by accident? (Most people were creating telescopes)
The first microscope was 6 feet long!!!
The Greeks & Romans used “lenses” to magnify objects over 1000 years ago.

4. Anthony van Leeuwenhoek
The History
Hans and Zacharias Janssen of Holland in the 1590’s created the “first” compound microscope
Anthony van Leeuwenhoek and Robert Hooke made improvements by working on the lenses
Robert Hooke
Anthony van Leeuwenhoek
Hooke Microscope

5. Caring for a Microscope
Clean only with a soft cloth/tissue
Make sure it’s on a flat surface
Don’t bang it
Carry it with 2 HANDS…one on the arm and the other on the base

6. Ocular lens (Eyepiece)
Body Tube
Nosepiece
Arm
Objectives
Stage
Stage Clips
Coarse Adjustment
Diaphragm
Fine Adjustment
Light
Always carry a microscope with one hand holding the arm and one hand under the base.
Base

7. Ocular Lens
Body Tube
Nose Piece
Arm
Objective
Lenses
Stage
Stage
Clips
Coarse Adj.
Diaphragm
Fine Adjustment
Light Source
Base
Skip to Magnification Section

8. Using a Microscope Start on the lowest magnification
Don’t use the coarse adjustment knob on high magnification…you’ll break the slide!!!
Place slide on stage and lock clips
Adjust light source (if it’s a mirror…don’t stand in front of it!)
Use fine adjustment to focus

9. Magnification
To determine your magnification…you just multiply the ocular lens by the objective lens
Ocular 10x Objective 40x:10 x 40 = 400
So the object is 400 times “larger”
Objective Lens have
their magnification
written on them.
Ocular lenses usually magnifies by 10x

10. Magnification
Multiply the eyepiece magnification (10X) by the objective magnification (4X, 10X, 40X)
Example: 4 x 10 = 40X total

11. Comparing Powers of Magnification
We can see better details with higher the powers of magnification, but we cannot see as much of the image.
Which of these images would be viewed at a higher power of magnification?

12. You do not need to use the stage clips when viewing wet-mount slides!
How to make a wet-mount slide …
1 – Get a clean slide and coverslip from your teacher.
2 – Place ONE drop of water in the middle of the slide. Don’t use too much or the water will run off the edge and make a mess!
3 – Place the edge of the cover slip on one side of the water drop.
4 - Slowly lower the cover slip on top of the drop.
Cover Slip
Lower slowly
You do not need to use the stage clips when viewing wet-mount slides!
5 – Place the slide on the stage and view it first with the red-banded objective. Once you see the image, you can rotate the nosepiece to view the slide with the different objectives.

13. KEEP BOTH EYES OPEN

14. PROBLEMS??