1. Products > Lipid-based In Vivo Transfection Kit
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > Lipid-based In Vivo Transfection Kit
Efficient in vivo delivery of nucleic acids and proteins is possible with the Altogen® Biosystems cationic lipid transfection reagent.
This product falls into the category of the versatile in vivo transfection kits that Altogen® Biosystems offers. Degradation of cargo molecules is minimized and delivery efficiency is maximized for any negatively charged molecules (DNA, RNA)
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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2. Products > Lipid-based In Vivo Transfection Kit
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > Lipid-based In Vivo Transfection Kit
Lipids refer to a class of molecules that occur naturally and include sterols, waxes, fats, vitamins that are fat soluble, monoglycerides, phospholipids and many others.
A positively charged head and one or two hydrocarbon chains encompass the structure of cationic lipids, various models of which are shown. The dynamic between the charged head and phosphate backbone of a nucleic acid allows for DNA condensation.
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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3. Summary Products > Lipid-based In Vivo Transfection Kit
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > Lipid-based In Vivo Transfection Kit
Summary
All Altogen® In Vivo Transfection Kits supplied with pre-optimized ready-to-run transfection protocols that eliminate the need for extensive transfection testing and optimization experiments
Lipid-based in vivo transfection reagent is a proprietary, animal-origin-free formulation of chemically engineered liposomes optimized for siRNA, shRNA, miRNA, plasmid DNA, and small protein in vivo delivery.
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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4. Mechanism of Action Products > Lipid-based In Vivo Transfection Kit
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > Lipid-based In Vivo Transfection Kit
Mechanism of Action
Cationic lipid reagents provide one of the most robust and effective tool for creating transfection complexes with negatively charged cargo (bind spontaneously to the liposomes that have a positive charge), while proprietary formulation allows to minimize complex size providing effective delivery into target tissues
The benefits of the lipid-based in vivo transfection kit include high transfection efficiencies into target tissues, pre-optimized protocol, easy to use, and consistent, reproducible results!
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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5. Product Details Products > Lipid-based In Vivo Transfection Kit
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > Lipid-based In Vivo Transfection Kit
Product Details
Lipid-based reagent
Complexes are stable in serum for 16 hours
Efficient delivery to the heart, lung, liver, pancreas, kidney, and multiple tumor types via systemic administration
Efficient siRNA and plasmid DNA delivery via IV, IT, or direct subcutaneous tumor injection (various tumor types)
Minimal toxicity
Functionally tested in mice and rats
Applicable for plasmid DNA/siRNA co-injection
Developed and manufactured by Altogen Biosystems
Modes of administration:
Systemic intravenous (i.v.) injection
Direct intratumoral (i.t.) injection
Intraperitoneal (i.p.) injection
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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6. Tissue-Targeting: Easiest Reagent to Use
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > Lipid-based In Vivo Transfection Kit
Tissue-Targeting: Easiest Reagent to Use
Combine diluted exogenous nucleic acid material and Altogen® Biosystems Lipid-based Transfection Reagent, vortex, incubate and choose administration route for targeted tissue
The experimental animal recipient, the targeted tissue and route of administration are the factors that will determine the amount of exogenous nucleic acids and suggested injection volume.
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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7. Product Details Products > Lipid-based In Vivo Transfection Kit
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > Lipid-based In Vivo Transfection Kit
Product Details
Our kits are easy to use and effective: Mix, incubate, dilute, and inject!
Our kits allow for sustained mRNA knockdown after a single siRNA injection
Target various organs through different administration routes.
There is very low in vivo toxicity and it is safe to use as there is no detectable inflammatory response.
Used for preclinical research worldwide!
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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8. Data Products > Lipid-based In Vivo Transfection Kit
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > Lipid-based In Vivo Transfection Kit
Data
Figure 1. Systemic administration (i.v.) of Lipid-based In Vivo reagent conjugated with siRNA targeting Lamin A/C mRNA or non-silencing control siRNA following the recommended protocol. Tissues were collected and RNA isolated 48 hours after first injection. Samples were analyzed by qRT-PCR for Lamin A/C gene expression levels. Ribosomal RNA levels were used to normalize the Lamin A/C data. Data are means ± SD (n=6).

9. Data Products > Lipid-based In Vivo Transfection Kit
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > Lipid-based In Vivo Transfection Kit
Data
Figure 2. Intratumoral administration (i.t.) of Lipid-based In Vivo reagent conjugated with siRNA targeting Lamin A/C mRNA or non-silencing control siRNA following the recommended protocol. Tissues were collected and RNA isolated 48 hours after first injection. Samples were analyzed by qRT-PCR for Lamin A/C gene expression levels. Ribosomal RNA levels were used to normalize the Lamin A/C data. Data are means ± SD (n=6).

10. Data Products > Lipid-based In Vivo Transfection Kit
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > Lipid-based In Vivo Transfection Kit
Data
Figure 3. Systemic administration (i.v.) of Lipid In Vivo Transfection Reagent conjugated with 80 ug of chemically modified siRNA targeting Lamin A/C mRNA or non-silencing control siRNA following the recommended protocol. Tissues (Liver, Brain, Muscle, Kidney, Tumor, Heart, Spleen, Lung) were collected and total protein fraction isolated 72 hours after first injection. Samples were analyzed by Western Blot Analysis for Lamin A/C gene expression levels.

11. Data Products > Lipid-based In Vivo Transfection Kit
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > Lipid-based In Vivo Transfection Kit
Data
Figure 4. Systemic administration (i.v.) of Lipid In Vivo Transfection Reagent conjugated with 80 ug of chemically modified siRNA targeting Lamin A/C mRNA or non-silencing control siRNA following the recommended protocol. Tissues (Liver, Brain, Muscle, Kidney, Tumor, Heart, Spleen, Lung) were collected and total protein fraction isolated 72 hours after first injection. Samples were analyzed by Western Blot Analysis for Lamin A/C gene expression levels.

12. Data Products > Lipid-based In Vivo Transfection Kit
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > Lipid-based In Vivo Transfection Kit
Data
Figure 5. Systemic administration (i.v.) of Lipid In Vivo Transfection Reagent conjugated with 80 ug of chemically modified siRNA targeting Lamin A/C mRNA or non-silencing control siRNA following the recommended protocol. Tissues (Liver, Brain, Muscle, Kidney, Tumor, Heart, Spleen, Lung) were collected and total protein fraction isolated 72 hours after first injection. Samples were analyzed by Western Blot Analysis for Lamin A/C gene expression levels.

13. Data Products > Lipid-based In Vivo Transfection Kit
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > Lipid-based In Vivo Transfection Kit
Data
Figure 6. Systemic administration (i.v.) of Lipid In Vivo Transfection Reagent conjugated with 80 ug of chemically modified siRNA targeting Lamin A/C mRNA or non-silencing control siRNA following the recommended protocol. Tissues (Liver, Brain, Muscle, Kidney, Tumor, Heart, Spleen, Lung) were collected and total protein fraction isolated 72 hours after first injection. Samples were analyzed by Western Blot Analysis for Lamin A/C gene expression levels.

14. Summary Products > Lipid-based In Vivo Transfection Kit
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > Lipid-based In Vivo Transfection Kit
Summary
Lipid-based reagent
Complexes are stable in serum for 16 hours
Efficient delivery to the heart, lung, liver, pancreas, kidney, and multiple tumor types via systemic administration
Efficient siRNA and plasmid DNA delivery via IV, IP, and direct subcutaneous tumor injection (various tumor types)
Minimal toxicity
Functionally tested in mice and rats
Applicable for plasmid DNA/siRNA co-injection
Developed and manufactured by Altogen Biosystems
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
Telephone   Fax   