1. Volume 19, Issue 9, Pages 1060-1069 (September 2017)
Assessment of biodistribution using mesenchymal stromal cells: Algorithm for study design and challenges in detection methodologies 
Blanca Reyes, Maria Isabel Coca, Margarita Codinach, María Dolores López-Lucas, Anna del Mazo-Barbara, Marta Caminal, Irene Oliver-Vila, Valentín Cabañas, Silvia Lope-Piedrafita, Joan García-López, José M. Moraleda, Cesar G. Fontecha, Joaquim Vives 
Cytotherapy 
Volume 19, Issue 9, Pages (September 2017)
DOI: /j.jcyt
Copyright © 2017 International Society for Cellular Therapy Terms and Conditions

2. Figure 1
Decision tree algorithm for the design of biodistribution studies. Critical points: (I) animal model selection (small or large) and route of administration (systemically or intended), (II) nature of cellular product (heterologous or homologous), (III) labeling (non-required, genetic or chemical) and follow-up (short or long term), and (IV) methodologies for detection: nucleic acid amplification (endpoint PCR or qPCR); IHC, bioimaging and MRI. BioD, biodistribution; PoC, proof of concept.
Cytotherapy  , DOI: ( /j.jcyt )
Copyright © 2017 International Society for Cellular Therapy Terms and Conditions

3. Figure 2
Challenges in the labeling of MSCs, processing of samples and analysis of cellular residence in target tissues. (A) Decay of eGFP expression along passage number in two ovine MSC lines (● and ■). (B) The Quality of DNA extracted using standard DNA extraction kits varied widely depending on the nature of each tissue. (C) Sensitivity of endpoint semi-quantitative PCR with regard to the number of eGFP-labeled MSCs (at 51.75% transduction efficiency). (D) T2-weighted MRI scan of a repaired vertebrae in which signal drop associated to MPIO-labeled AF-oMSCs is depicted in the repaired region as a dark hypointense area (black arrows). (E) Similar hypo intensities were detected in hemorrhagic regions thus masking the presence of intact labeled MSCs (white arrow) and blue color after PERL's staining (inset). (F) Histologic section of a femoral head that was decalcified and paraffinized before detecting eGFP-labeled after IHC procedures with antibodies specific to GFP. (G) A human MSC was detected in the liver of a mouse by using anti-human mitochondria antibodies (nuclei were counterstained with FastGreen). (H) Sensitivity of eGFP detection by bioimaging and (I) presence of eGFP in livers from mice treated with eGFP-labeled human MSCs. Scale bars = 100 µm. FLI, fluorescence imaging.
Cytotherapy  , DOI: ( /j.jcyt )
Copyright © 2017 International Society for Cellular Therapy Terms and Conditions