1. Nuclear Medicine and Biology
Intraarticular and intravenous administration of 99MTc-HMPAO-labeled human mesenchymal stem cells (99MTC-AH-MSCS): In vivo imaging and biodistribution 
Luis Meseguer-Olmo, Antonio Jesús Montellano, Teresa Martínez, Carlos M. Martínez, Beatriz Revilla-Nuin, Marta Roldán, Cristina Fuente Mora, Maria Dolores López-Lucas, Teodomiro Fuente 
Nuclear Medicine and Biology 
Volume 46, Pages (March 2017)
DOI: /j.nucmedbio
Copyright © 2016 Elsevier Inc. Terms and Conditions

2. Fig. 1
Scintigraphic images acquired after IV administration of 17.3± radiolabeled ahMSCs (8.1±0.5Bq cell−1) to mature New Zealand male rabbits anesthetized by (IM) injection of hydrochloride of clorpromazine, 10mgkg −1; hydrochlorate of ketamine, 50mgkg−1 and xylazine 5mgkg−1. a) Dynamic images of 30 s/frame during 25min, in which we can see the injection site and lung uptake. b) Lung, liver, spleen, intestinal and urinary track uptakes in 6h postinjection planar images.
Nuclear Medicine and Biology  , 36-42DOI: ( /j.nucmedbio )
Copyright © 2016 Elsevier Inc. Terms and Conditions

3. Fig. 2
Scintigraphic images obtained after intraarticular injection of 17.6± radiolabeled ahMSCs (7.0±2.7Bq cell−1) to mature New Zealand male rabbits, anesthetized by IM injection of hydrochloride of clorpromazine, 10mgkg −1; hydrochlorate of ketamine, 50mgkg−1 and xylazine 5mgkg−1. a) Dynamic images of 30s/frame during 25min in which we can see only injection site. b) Static studies six hours post injection revealed intraarticular, heart, spleen, liver, intestinal and urinary tract uptake, with no lung uptake.
Nuclear Medicine and Biology  , 36-42DOI: ( /j.nucmedbio )
Copyright © 2016 Elsevier Inc. Terms and Conditions

4. Fig. 3
Real-Time PCR from organs from a rabbit 24h post inoculation of human mesenchymal stem cells (ahMSCs). Only the knee was positive for human GAPDH gene. All other organs were negative.
Nuclear Medicine and Biology  , 36-42DOI: ( /j.nucmedbio )
Copyright © 2016 Elsevier Inc. Terms and Conditions

5. Fig. 4
a: Representative image of a tibial sinovial membrane from a rabbit 24h post inoculation of human mesenchymal stem cells (ahMSCs). An acute fibrinous inflammatory infiltrate (f) could be evidenced over a reactive synovial epithelium (asterisk) (f). Beneath the basal membrane, many adipocytes (a) could be also observed. Hematoxylin and eosin (H&E) technique. Scale bar: 100μm. b: Detail of image 1a. The acute inflammatory fibronous infiltrate (f) is composed mainly of polymorphonuclear neutrophils (head arrows), which are also infiltrating the reactive synovial epithelium. Hematoxylin and eosin (H&E) technique. Scale bar: 50μm.
Nuclear Medicine and Biology  , 36-42DOI: ( /j.nucmedbio )
Copyright © 2016 Elsevier Inc. Terms and Conditions

6. Fig. 5
a: Representative image of a tibial sinovial membrane from a rabbit 24h post inoculation of human mesenchymal stem cells (ahMSCs). The epithelial surface is covered by a fibrinous-like membrane (f) in which many cells can be evidenced (head arrow). The synovial epithelium is reactive (asterisks), and remains on a connective tissue layer (C). Hematoxylin and eosin (H&E) technique. Scale bar: 100μm. b: Detail of the image 2a. A deeper analysis reveal that the inflammatory infiltrate is composed by polimorphonuclear neutrophils (head arrows) interspersed both in the fibrinous infiltrate (f) and between reactive epithelial cells (asterisks). The synovial epithelium remains on a connective tissue layer (C). Hematoxylin and eosin (H&E) technique. Scale bar: 50μm.
Nuclear Medicine and Biology  , 36-42DOI: ( /j.nucmedbio )
Copyright © 2016 Elsevier Inc. Terms and Conditions

7. Fig. 6
a: Representative image of distribution of human CD90 in tibial sinovial membrane from a rabbit 24h post inoculation of human mesenchymal stem cells (ahMSCs). There is positive reaction in the reactive sinovial epithelium layer (asterisk). Immunohistochemical ABC procedure anti-human CD90 antigen. Scale bar: 100μm. b: Detail of the image. There is positive immunoreaction against human CD90 antigen in cells infiltrating the reactive epithelium (head arrows). Immunohistochemical ABC procedure anti-human CD90 antigen. Scale bar: 50μm.
Nuclear Medicine and Biology  , 36-42DOI: ( /j.nucmedbio )
Copyright © 2016 Elsevier Inc. Terms and Conditions