1. The Microscope

2. History… 1590 Hans and Zacharias Jensen Dutch eyeglass makers
Designed first microscope
1600’s Leeuwenhoek
Made lenses as a hobby
Made over 250 microscopes
Made microscope that could magnify 300 times
First to see living organisms “animalcules”

3. Tools to view specimen-
1. Hands lens (magnifying glass)- magnifies 3-5x

4. Tools to view specimens-
2. Stereoscope (dissecting scope)- magnifies 10-60x; shines light onto the specimen; solid objects

5. Tools to view specimens-
3. Light microscope (compound)- magnifies up to 2,000x (ours 400x), light passes through the specimen and then through 2 or more lenses

6. Tools to view specimens-
Scanning electron microscope (SEM)- 50,000x; surface of specimen coated with metal; electrons bounce
off and 3D image
is recorded

7. Tools to view specimens-
5. Transmission electron microscope (TEM)- 2,000,000x; specimen has to be sliced thin; electrons pass through it; 2D image is recorded

8. Total Magnification:
Light microscope- eyepiece x objective lens = total mag Example: 10 (always) x 40 = 400x