1. The wacky history of the cell theory

2. Microscopy
Janssen’s microscope (1590’s)
capable of magnifying images approximately three times when fully closed and up to ten times when extended to the maximum.

3. Van Leeuwenhoek “The Father of Microbiology”
first person to observe and describe single celled organisms (microorganisms)
first to record and observe muscle fibers, bacteria, spermatozoa and blood flow in capillaries (small blood vessels).

4. Robert Hooke (1665)
Coined the term “cell”

5. our compound microscopes are called light microscopes
parts of microscope (diagram sheet) and their functions
lenses:
1. eyepiece lens: 10x

6. 2. objective lenses:
very low: 5x (total=50X)
low: 10x (total= 100X)
high: 43x (total=430X)
oil: 97x (total=970X)

7. light microscopes limited to 2000x
why? The wave length of light only allows us to see object at 2000X or less.
resolution drops with increase in magnification.

8. resolution= the ability to see detail in a in/on an object under the microsope
unit of measure = μ micron (SI unit of length measurement)
= m
1 millimeter = 1,000 microns

10. TEM’s: Transmission Electron Microscopes
use electrons in place of light
focus with magnets
electron beam passes through thin section of object (therefore not alive)
magnifies 200,000x: photo enlarged 5x: total= 1,000,000X
(no resolution problems)

11. Transmission Electron Microscope (TEM)

12. SEM’s: Scanning Electron Microscopes
shows surface of object in 3D
allows very high magnification

13. Scanning electron microscopes (SEM)

14. Match the image with the type of microscope that produces itB C
Light microscope
Scanning electron microscope
Transmission electron microscope