1. LEVENT TÜMKAYA, GÜLŞAH BALIK, TOLGA MERCANTEPE, YEŞİM BAYOĞLU TEKİN
The effect of bilberry in the ovary Cisplatinin toxicity
LEVENT TÜMKAYA, GÜLŞAH BALIK, TOLGA MERCANTEPE, YEŞİM BAYOĞLU TEKİN
15. TJOD KONGRESİ, 21 Mayıs 2017

2. THE EFFECT OF BİLBERRY İN THE OVARY CİSPLATİNİN TOXİCİTY
Chemotherapeutic agents cause early menopause and amenorrhea
with the loss of primordial follicles in ovary tissue.
Cisplatin (Cis) induces reproductive toxicity
with the formation of free oxygen radicals like superoxide and hydroxyl radicals.

3. Bilberry has been stated that
THE EFFECT OF BİLBERRY İN THE OVARY CİSPLATİNİN TOXİCİTY
Bilberry has been stated that
it has a protective effect against the increase in the oxidative stress parameters and it has considerably prevented ovary toxicity.,
In this study, we aimed to show
the effectiveness of bilberry in reducing the toxic effect of cisplatin in the ovary.

4. Material and Method: Spraque Dawley albino kind rats (3-5 months ’),
Totally, 48 mature female,
Spraque Dawley albino kind rats (3-5 months ’),
Weights were ranging from gr were used.
All animals were looked after and fed at 22±3 C° room temperature
with 55-60% humidity rate in a sterile 12 hours bright and 12 hours dark experimental animals unit environment.

5. Rats were randomly seperated in 6 groups (Table 1).
Table 1.All the groups in the study and application carried on groups
GROUP NAME
NUMBER OF RATS
GROUP 1: Control : no application was done 8
GROUP 2: DISSOLVER CONTROL (distil water +ethanol )
Intraperitoneal bilberry dissolver containing distil water and ethanol was given for 8 days .
GROUP 3: SHAM( Bilberry MG CONTROL) Only Intraperitoneal bilberry extract , dissolver containing 100 mg/kg distil water and ethanol were given for 8days.
GROUP 4: CİSPLATİN, a single 16mg/kg intraperitoneal cisplatin dose was given for 5 days .
GROUP 5: CİSPLATİN+100 MG BILBERRY:Every day , i.p.100 mg/kg bilberry was given for eight days and on the fifth day, a single dose of intraperitoneal 16 mg/kg cisplatin was given .
GROUP 6: CİSPLATİN+200 MG BILBERRY: Every day i.p.200 mg/kg bilberry was given for 8 days and on the fifth day a single dose of intraperitoneal 16 mg/kg cisplatin was given.

6. Material and Method: THE PREPARATİON OF BİLBERRY EXTRACT :
The bilberry extract [Bilberry (vaccinium myrtillus) Herbal Liquids, Health Aid, England] prepared to involve 330mg bilberry extract in 1 ml with half –and-half ethanol as a supporter product and distillate water and by diluting distillate water and half –and- half ethanol again under sterile conditions were prepared to be 50mg in 1ml.
HİSTOPATHOLOGİC RESEARCH:
Ovary tissue was fixed in the 10% formalin solution for 48 hours,
buried in paraffin blocks,
The preparations were examined under light microscope
(Leica DM6200-Germany) and they were photographed with Olympus DP20 camera.

7. Material and Method: STEOROLOGY ANALYSİS:
In 60 different chosen neutral zones, by using Stereoinvestigator 9.0 program, the numeral density of primordial and preantral/antral follicles for all the groups.
Stereoinvestigator 9.0 consists of a light microscope where a camera was placed, a system involving a microscope plate and a computer with a software.
The numeral density data of follicles were calculated by using SPSS (IBM, New York, A.B.D.) program.
Single-track ANOVA was applied to the data.
They were evaluated by Duncan test.
P <0.05 was statistically significant.

8. FİNDİNGS: We observed that the ovary tissue which belongs to
the control group and Sham group and the follicles were in a normal structure.
Statistically, we didn’t find a difference between bilberry group and the control group.
In the ovary tissues of animals that cisplatin was applied,
we tracked apoptotic cells which have pyknotic nucleous structure, oedematous regions common in ovary stroma and capillary congestion.
We determined preantral/antral follicles and degenerative primordial in the ovary cortex.
In the Cis +Bilberry 100mg group, we found a decline (p<0.05) (Table 2) in the number of apoptotic cells and degenerative follicles in comparison with cisplatin group.
The results of the groups that Cis +Bilberry 100mg and 200mg were applied were similar.

9. Cisplatin+Tea grape 100mg Cisplatin+ Tea grape 200mg
Table 2. Stereological Analysis Results (Mean ± Standard Deviation)
Gropus
Degenerative
Primordial Follicle
Numerical Density
(number/mm2)
Degenerative antral/preantalFollicle
Control
0.75±0.71c
1.75±0.70c
D. Water+Ethanol
1.13±0.64b,c
1.5±0.93b,c
Sham
1.25±0.46b,c
1.37±0.92b,c
Cisplatin
5.50±1.60a
8.62±1.84a
Cisplatin+Tea grape 100mg
2.63±1.40c
2.50±0.92c
Cisplatin+ Tea grape 200mg
2.25±1.03c,d
2.25±1.04c,d
aControl group versus p<0.0.5 bControl group versus p>0.0.5 cCisplatin group versus p<0.0.5 dCisplatin+Billberyy 100mg group versus p>0.0.5

10. WOMEN’S REPRODUCTİVE FUNCTİONS WİLL BE PROTECTED,
DİSCUSSİON
As a result,
BİLBERY DİMİNİSHED BOTH THE NUMBER OF APOPTOTİC CELLS AND DEGENERATİVE FOLLİCLES.
DURİNG CHEMOTHERAPEUTİC TREATMENT, WİTH THE SUPPORTİVE CARE LİKE BİLBERRY,
WOMEN’S REPRODUCTİVE FUNCTİONS WİLL BE PROTECTED,
THE DEGENERATİVE EFFECT ON OVARY WİLL BE REDUCED BY İNCREASİNG ANTİOXİDANT İMPACT.