1. Dr Michael Considine

2. Genomics of Cabernet clones
Why?
Grapevine propagated by cuttings, accumulation of random mutations through repeated propagation leads to identification of unique clones.
Clones represent a major means of development and differentiation of wine quality and style.
Currently there is limited knowledge on underlying genetic changes contributing to clonal variation.

3. Genomics of Cabernet clones
Why?
By determining how clones vary between and within each other, this project will provide industry with the tools to identify which clones are likely to be more stable producers of high quality wine.

4. Genomics of Cabernet clones
Why Cabernet?
The ‘regional hero’ of key Australian premium wine regions; Margaret River, Coonawarra…
Cash contributions to this project WA-based.
WA Cabernet industry originally based on clonal selections from Houghton Wines, but currently dominated by one clone; SA126 (~70%).
Significant interest in replanting.

5. Genomics of Cabernet clones
Why genomics?
Rapid advances in scale, speed and cost make genomic technology readily available to tailored applications in agriculture.
The genomic road map for grapevine will soon be based on Cabernet (USDA8) – some data already available.

6. Genomics of Cabernet clones
Project goals:
Define genomic basis of difference in several elite Cabernet clones from WA and SA industries. Define markers for fingerprinting.
Dissect the influence of virus and epigenetics on clonal identity.
Explore clonal differences in genes coding for fruit quality.

7. Genomics of Cabernet clones
Expected outcomes:
Markers to discriminate clones of Cabernet Sauvignon.
Tools to identify existing and future clones with genetic potential to produce high quality wine.
A platform to explore how and why clones perform differently in different regions or seasons.
Data made available for future goals.

8. Genomics of Cabernet clones
Origin
SA125
Yalumba Nursery, SA
Reynella (m.s.)
CW44
PDFs
WA Cape (Htn, m.s.)
Yalumba Nursery, SA, ex WA
ENTAV338
Yalumba Nursery, ex France
SA126 (vine 1)
WAVIA foundation, Manj, WA
SA126 (vine 2)
SA126 (vine 3)
Houghton 5#
Marg River, WA
Houghton 13#
Houghton 16#
SA126#
Houghton
Gingin, WA
Houghton
Houghton

9. Whole-genome sequencing:
Genomics of Cabernet clones
Whole-genome sequencing:
Short reads, high depth, >100 million reads per individual.
Coverage >95% Cabernet genome (USDA8).
>30,000 unique sequence variants among individuals.

10. Genomics of Cabernet clones
100 50
CW44 (Solar)
SA126 (Grayln)
SA126 plant 3 (Manjimup)
SA126 plant 2 (Manjimup)
SA126 plant 1 (Manjimup)
SA125 (Solar)
Reynella (Solar)
PDFS (Solar)
Hougton a (Moondah)
Hougton b (Moondah)
Hougton c (Moondah)
Hougton 5 (Grayln)
Hougton 16 (Grayln)
Hougton 13 (Grayln)
ENTAV338 (Solar)
WA Cape (Solar)
Depth
Coverage
Coverage Soap
Percentage

11. Whole-genome sequencing:
Genomics of Cabernet clones
Whole-genome sequencing:
All individuals recognisably Cabernet 

12. Current challenges: Sequence variation between replicate vines.
Genomics of Cabernet clones
Current challenges:
Sequence variation between replicate vines.
Plan to repeat sequencing of WAVIA SA126 – three vines, three samples per vine – to establish sources of ‘error’.
Investigation of modes of mutation, particular mobile elements requires increased computing power.
Planning application to the state Pawsey Supercomputer facility.
Influence of virus expression part of a PhD project in table grapes.

13. Thank you UWA Dr Patri Agudelo-Romero Prof Ryan Lister
Genomics of Cabernet clones
Thank you
UWA
Dr Patri Agudelo-Romero
Prof Ryan Lister
Prof Dave Edwards
Prof Jacqui Batley
Dr Philipp Bayer
Wisam (Sam) Salo
DAFWA
Richard Fennessy
Glynn Ward
WAVIA
Jim Campbell-Clause
AWRI
Paul Chambers
Yalumba
James Freckleton

14. Thank you Additional in kind support Financial support Yalumba Nursery
Genomics of Cabernet clones
Thank you
Additional in kind support
Yalumba Nursery
Australian Wine Research Institute
Accolade
Credaro
Ferngrove
Flametree
Plantagenet
Vasse Felix
Financial support
DAFWA
WAVIA
AHA Viticulture
ARM (year 1)
Cape Mentelle
Cullen Wines
Devils Lair
Howard Park
Moss Wood
Voyager Estate
Woodlands
Xanadu