2. AGROBIOLOGICAL
RESEARCH
AND CONSULTING

4. FUNGAL DISEASES CAN RESULT IN CONSIDERABLE DAMAGE TO AGRI-CULTURAL AND HORTICULTURAL CULTIVARS.

5. UNDER CERTAIN CIRCUMSTANCES AND WITH AN ACCORDING EPIDEMIC A LOSS IN YIELD OF 80 % IS POSSIBLE.

6. HOW CAN THE HARVEST BE PROTECTED ?
MECHANICAL CULTIVATION
USE OF RESISTANT CULTIVARS
USE OF EFFECTIVE PESTICIDES

7. FUNGAL DISEASES ADAPT TOWARDS PESTICIDES AND RESISTANCE GENES JUST LIKE HUMAN PATHOGENS CAN ADAPT TOWARDS ANTIBIOTICS.

8. THIS SUBJECT AREA IS OUR EXPERTISE:

9. DISEASE PATHOGENS ACTIVE INGREDIENT CULTIVAR
HAS THE PATHOGEN ADAPTED OR BECOME RESISTANT TO THE FUNGICIDE ?
IS THERE AN ADAPTION TOWARDS THE RESISTANCE OF A CULTIVAR ?
WHAT STRATEGIES CAN BE APPLIED IN CASE OF AN ADAPTION ?
ARE NEW BREEDING LINES AVAILABLE THAT SHOW BETTER RESISTANCE ?
ACTIVE INGREDIENT
CULTIVAR

10. EVERY YEAR WE SAMPLE PATHOGEN SPORES ACROSS EUROPE TO ANSWER THESE QUESTIONS.

11. ST. MARY ON SEA, ENGLAND

12. ALSACE, FRANCE

13. SCOTTISH BORDER

14. MARCHFELD, AUSTRIA

15. LOCH NESS, SCOTLAND

16. RHINELAND, GERMANY

17. CAP BLANC NEZ, FRANCE

18. OF COURSE NOT ONLY IN PRETTY PLACES!
EASTCOAST, SCOTLAND

19. THE PATHOGEN STRAINS ARE THEN ISOLATED IN THE LABORATORY AND STORED.

20. WE APPLY DIFFERENT METHODS TO ANALYSE THE PATHOGEN STRAINS:

21. ON LIVING PLANT MATERIAL in vivo
ACTIVE
COMPOUND
PATHOGEN
PLANT
PATHOGEN
PLANT

22. UNDER ARTIFICIAL CONDITIONS in vitro
PATHOGEN
COMPOUND

23. MOLECULAR- BIOLOGICAL METHODS
undigested
sensitive/ItaI
resistant/ItaI
MOLECULAR-
BIOLOGICAL
METHODS
(EpiGene)

24. FINALLY, WE EVALUATE THE OBTAINED DATA.

25. PROBIT-ANALYSIS EC50 20 40 60 80 100 Contr. 0,03 0,1 0,3 1,0 3,0 10,020 40 60 80
100
Contr.
0,03
0,1
0,3
1,0
3,0
10,0
30,0
CONCENTRATIONS (mg/l)
% GROWTH COMPARED TO UNTREATED CONTROL
EC50

26. QUALITATIVE RESISTANCE
Selection
Selection
sensitive individuals
resistant individuals

27. DHPLC-PCR (EpiGene) 100% WM sensitive 50% WM resistant2 3 4 5
Minutes

28. TAQMAN PCR
(EpiGene)

29. WE PROVIDE OUR PARTNERS WITH OUR KNOWLEDGE AND INFORMATION:

30. PLANT BREEDING

31. PLANT
BREEDING
CHEMICAL INDUSTRY

32. MECKLENBURG-VORPOMMERN RHINELAND-PALATINATE
PLANT
BREEDING
ADVISORY SERVICES
CHEMICAL
INDUSTRY IN
SCHLESWIG-HOLSTEIN
LOWER SAXONY
MECKLENBURG-VORPOMMERN
HESSE
THURINGIA
SAXONY
RHINELAND-PALATINATE
BADEN-WÜRTTEMBERG
BAVARIA

33. MECKLENBURG-VORPOMMERN RHINELAND-PALATINATE
ADVISORY
SERVICES IN
SCHLESWIG-HOLSTEIN
LOWER SAXONY
MECKLENBURG-VORPOMMERN
HESSE
THURINGIA
SAXONY
RHINELAND-PALATINATE
BADEN-WÜRTTEMBERG
BAVARIA
PLANT
BREEDING
CHEMICAL
INDUSTRY

34. SO, IF YOU EVER SEE ...
... THIS VEHICLE:
... ANYWHERE IN EUROPE ...

35. DON‘T BE SURPRISED !?!
´CAUSE YOU‘VE JUST MET
THE SPOREHUNTERS !
JUST GIVE US A HIGH FIVE !

36. IN THE NAME OF A MODERN, EFFICIENT AND SUSTAINABLE AGRICULTURE
 EpiLogic
created by B. Jaser