1. Surface-Plasmon-Mediated IR Transmission of Metal Microarrays for the
Molecules of the Cell Membrane
Surface Plasmon Basics
Enhanced IR Absorption Spectroscopy
DPPC, Cholesterol, Gramicidin
Shaun M. Williams, Kenneth R. Rodriguez, Shannon Teeters-Kennedy, Katherine Cilwa, Dan Meleason, Summit Shah, Alexandra Sudnitsyn, Frank Hrovat, Smruti Patel, and James V. Coe
OSU International Molecular Spectroscopy Symposium, June 21, 2005, Columbus OH

2. Shannon Teeters- Kennedy
Coe Group
Shaun Williams
Kenneth Rodriguez
Shannon Teeters- Kennedy
Katie Cilwa
Dan Meleason
CHE
Jim Coe (PI)
Summit Shah
Alexandra Sudnitsyn
Frank Hrovat
Smruti Patel
38502-ACS
Matt Young
Mandy Stafford
Sarah Bishop
Ushani Lincoln
Kerry Katz
Keith Zomchek
Trisha Rogers

3. Ni Mesh Shiny Side Dull Side 12.7mm 6.5mm 6.5mm 3 mm thick
Precision Eforming, LLC 839 Route 13 Cortland, NY Sales Phone: (607) Fax: (607)
6.5mm
6.5mm
3 mm thick
Dull Side

4. Ebbesen’s Extraordinary Transmission1 Effect in the IR
SEM of Precision Eforming Ni Mesh
12.7mm
6.5mm
6.5mm
5 mm thick
1 Ebbesen, T.W., Lezec, H.J., Ghaemi, H.F., Thio, T. and Wolff, P.A. Nature, 391 (1998) 667.
2 Bethe, H.A. Phys. Rev, 66 (1944) 163.
Williams et al., J. Phys. Chem. B (2003)

5. 12.7mm
6.5mm
6.5mm
5 mm thick
Larson

6. IR h

8. _ + + _ Glycerol subunit double bond cholesterol DPPC
1-palmitoyl-2-oleoyl-PC
1,2-di-palmitoyl-glycero-3 phosphocholine
16:0/18:1
16:0/16:0

9. Self Assembled Photonic Crystals
metal S S S S S S S S S
metal S S S S S S S S
Self Assembled Photonic Crystals

10. S S S S S S S S S S
metal S S S S S S
metal S S S S S S S S S S S S

11. S S S S S S S S S S
metal S S S S S S
metal S S S S S S S S S S S S

12. SAMs
Self-Assembled Monolayers

13. Alkanethiolate SAMS 10 mm
FTIR transmission spectra of a Cu-deposited mesh (dotted, upper trace) upon coating with a SAM of 1-dodecanethiol (solid, black trace).
Williams et al., Nanotechnology, 15 S495-S503 (2004)

14. CH2 Wagging Progressions
Ratio to RAIRS (Laibinis et al JACS ’91, 85o incident, 18o tilt, 2.2 nm thick)  16 m pathlength
Rodriguez et al., J. Chem. Phys (2004)

15. More Complex Assemblies for Sensors and Nanotechnology

16. S S S S S S S Williams et al., Nanotechnology, 15 S495-S503 (2004)
Transmission spectra of a bare Cu-deposited mesh (top tace) that was coated with a hexadecanethiol SAM (middle) and then further coated with a DPPC phospholipid bilayer (bottom). Note the shift in transmission resonances (analogous to ATR) and the appearance of molecular absorptions (circled) upon coating.
Williams et al., Nanotechnology, 15 S495-S503 (2004)

17. DPPC
metal
glycerol
chiral carbon
trilayer
monolayer

18. DPPC:Cholesterol

19. Membrane Bound Protein - Gramicidin
crystal
membrane

20. C=O stretch, 16 different stretches, Amide I bands

21. Williams et al., Nanotechnology, 15 S495-S503 (2004)
PM3 optimized Gramicidin A monomer (membrane structure). 15 amino acids, alternating L and D, coil into a nanochannel. A single-stranded, helical dimer (HD) spans a membrane allowing ion transport. O atoms (dark) of C=O groups line the ion channel.
Williams et al., Nanotechnology, 15 S495-S503 (2004)

22. Molecules of the Cell Membrane
Summary:
Enhanced IR Absorption Spectroscopy
Phase of DPPC in Bilayers
Cholesterol
Membrane Bound Protein - Gramicidin
Stacked Sensors