1. Volume 194, Issue 1, Pages 245-251 (July 2015)
Down-Regulation of SLC8A1 as a Putative Apoptosis Evasion Mechanism by Modulation of Calcium Levels in Penile Carcinoma 
Juan J. Muñoz, Sandra A. Drigo, Mateus C. Barros-Filho, Fábio A. Marchi, Cristovam Scapulatempo-Neto, Gustavo S. Pessoa, Gustavo C. Guimarães, José Carlos S. Trindade Filho, Ademar Lopes, Marco A.Z. Arruda, Silvia R. Rogatto 
The Journal of Urology 
Volume 194, Issue 1, Pages (July 2015)
DOI: /j.juro
Copyright © 2015 American Urological Association Education and Research, Inc. Terms and Conditions

2. Figure 1
A, SLC8A1 relative expression detected by RT-qPCR in PeCa and normal penile (N) samples. Transcript expression is shown in log scale (Mann-Whitney test p = 0.001). B, SLC8A1 gene mapped at 2p21 and 1 representative case showing SLC8A1 locus covered by 8 probes with no copy number alteration on array-CGH. C, miR-223 relative expression in PeCa and normal penile samples (Mann-Whitney test p = 0.002). D, correlation analysis of SLC8A1 and miR-223 transcript levels in penile tumors with transcript expression shown in log scale (Spearman test p = 0.015).
The Journal of Urology  , DOI: ( /j.juro )
Copyright © 2015 American Urological Association Education and Research, Inc. Terms and Conditions

3. Figure 2
Matched freshly frozen normal penile tissue sample from case 1, PeCa samples from cases 1 and 3, and sample of nonneoplastic tissue adjacent to PeCa (Adj) from case 3. A, immunostaining for cleaved caspase-3 and Ki-67. H&E, reduced from ×4. Dotted lines indicate higher magnification of squamous cells selected for further laser ablation for calcium and sodium analysis. H&E, reduced from ×200. B, LA(i)-ICP-MS reveals 44Ca+ and 23Na+ distribution normalized to 13C+ (44Ca+/13C+ and 23Na+/13C+, respectively). Color bars indicate signal intensity. Blue areas represent low ion distribution. Red areas represent high ion distribution. C, immunostaining for Ki-67 and cleaved caspase-3. Reduced from ×400.
The Journal of Urology  , DOI: ( /j.juro )
Copyright © 2015 American Urological Association Education and Research, Inc. Terms and Conditions