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Published byBlaise Jackson Modified over 6 years ago
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Draq5
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Main Features In vitro research purposes only
Capable of stoichiometric binding to DNA inside living and fixed cells Rapid uptake into living cells (concentration range 5-10 µM x 1-5 mins; at room temp. or 37°C)
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Basic features nuclear DNA live or fixed mammalian cells with minimal RNA-associated fluorescence Emission spectrum beyond 670 nm providing minimal overlap with the emissions from visible range dyes including GFP
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Flowcytometry DRAQ5™ can provide cell cycle information
(eg %G0/1, %S, %G2/M) in mixed populations identified by FITC labeling. The quality of analysis can approach that of traditional propidium iodide / ethidium bromide techniques Rapid staining of cells in seconds by simple addition of DRAQ5™ to buffer or culture medium with no need to wash prior to imaging It is not recommended for viable cell sorting since the persistence of DRAQ5™ on cellular DNA is ultimately cytotoxic
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