1. From: Molecular Mechanisms Underlying the Filtration Bleb-Maintaining Effects of Suberoylanilide Hydroxamic Acid (SAHA)
Invest. Ophthalmol. Vis. Sci ;58(4): doi: /iovs
Figure Legend:
Effects of SAHA on viable cell numbers and cell toxicity. (A) HConFs were pretreated with SAHA (1–5 μM) for 1 hour and stimulated with TGF-β2 for 48 hours. Viable cell numbers were evaluated by WST-8 assay. Compared with the control, TGF-β2 significantly increased the viable cell number, which was significantly attenuated by pretreatment with SAHA in a dose-dependent manner. *P < 0.01 compared with control, #P < 0.01 compared with TGF-β2. Data are shown as means ± SE. (B) Under the same conditions, cytotoxic effects of SAHA were evaluated by double-staining with Hoechst (blue, live and dead cells) and PI (red, dead cells). PI-positive cells were barely detectable after treatment with SAHA at the doses tested in the present study. Cells were treated with 2 mM H2O2 for 4 hours as a positive control. Scale bar denotes 500 μm.
Date of download: 10/5/2017
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