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The mating type genes of Melampsora larici-populina

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Presentation on theme: "The mating type genes of Melampsora larici-populina"— Presentation transcript:

1 The mating type genes of Melampsora larici-populina
Hélène Niculita Hirzel The mating type genes of Melampsora larici-populina 20-21 August 2008, Nancy

2 Melampsora larici-populina life cycle
(larch needles) Host (poplar) 20-21 August 2008, Nancy Mating types genes in Melampsora

3 Mating process Mating pheromone response pathway meiosis hyphal growth
a hyphal growth nuclear fusion cell fusion 20-21 August 2008, Nancy Mating types genes in Melampsora

4 Mating pheromone response pathway
cdc25 Ste6 Gpa1 β γ GDP Receptor GTP Pheromone HMG P Ras1 MAPKKK MAPKK MAPK ACTIVATION cdc24 Cdc42 Ste20 MORPHOLOGY Nucleus Cytoplasm Plasma Membrane pheromone receptor mating type transcription factor genes Mating Response Genes Conserved between ascomycetes and basidiomycetes 20-21 August 2008, Nancy Mating types genes in Melampsora

5 Tetrapolar system Two unlinked loci determined the mating type in 60% of Basidiomycetes A locus: HD1 HD2 a pair of divergently transcribed homeodomain transcription factor STE3 Ph B locus : - lipopeptide pheromones - G-protein-coupled pheromone receptor 20-21 August 2008, Nancy Mating types genes in Melampsora

6 The mating-types genes in Mlp
7 TM, EST 7 TM 5 TM 20-21 August 2008, Nancy Mating types genes in Melampsora

7 Genomic features of the mating-types surrounding regions
20-21 August 2008, Nancy Mating types genes in Melampsora

8 Genomic features of the mating-types surrounding regions
20-21 August 2008, Nancy Mating types genes in Melampsora

9 Conservation of the mating-types surrounding regions
20-21 August 2008, Nancy Mating types genes in Melampsora

10 Conservation of the mating-types surrounding regions
20-21 August 2008, Nancy Mating types genes in Melampsora

11 Conclusions and Perspectives
Melampsora larici-populina genome present the A and B mating type genes No synteny is observed between the regions surrounding the A and B loci of Melampsora larici-populina and Puccinia graminis Experimental investigations are needed to confirm the tetrapolar mating-type system of Melampsora larici-populina and to identify “the B locus”. 20-21 August 2008, Nancy Mating types genes in Melampsora

12 Acknowledgements University of Lausanne Ian Sanders INRA Nancy, France
Francis Martin Sébastien Duplessis 20-21 August 2008, Nancy

13 Fungi phylogeny Glomeromycota (Glomus, endo- mycorrhizae)
20-21 August 2008, Nancy Mating types genes in Melampsora


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