1. Center for Cellular and Molecular Biology, Hyderabad
Biology of Mesenchymal Stem Cells from various sources and their potential use in Tissue Engineering
Rekha Tripathi, Udayachandrika.K, M. Amarnath, Sujata Reddy, Dr. Shashi Singh
Center for Cellular and Molecular Biology, Hyderabad
CHARACTERIZATION
ABSTRACT
ABSTrACT
IMMUNE RESPONSE OF MSCS
We present a comparison of immune-properties of MSC isolated from PL, AD, WJ and BM.These study suggest that MSCs from PL,AD,WJ could be substituted for BMSCs.
Adult stem cells are maintained in various tissues in a quiescent state, which is believed to protect them from various stresses and transformations. The basic stem cell traits are defined as the cell’s capacity to divide indefinitely and plasticity - the ability to differentiate into many different cell types. Adult stem cells are being derived from various sources- placenta, Wharton’s jelly, cord blood, bone marrow, lipo-aspirate and characterized in our laboratory. All the sources yield a large number of highly potential mesenchymal stem cell that expand in cultures; have markers defined for MSC and differentiate into osteogenic, chondrogenic, neurogenic and endothelial lineages when induced. These MSC do not elicit any proliferative response to allogeneic lymphocytes in culture conditions; they do not release any cytokines that might support the proliferative activity and show immune suppressive effects when injected to immune-competent mice. The proliferation capacity of MSC differ slightly. MSC derived from placenta and Wharton’s jelly show longer survival in cultures up to 16 passages whereas the ones derived from bone marrow, cord blood or adipose rarely go beyond 6 passage and age rapidly. These cells are being used to study effects of niche modifications using nanostructures.
Immune suppression is induced by MSC derived from different sources both in vitro and in vivo
MESENCHYMAL STEM CELLS
AGEING OF MSC
STUDY OF DIFFERENTIATION
Growth curve of MSC from different sources
Characterization of MSC
Immune Response of MSC
Study of Ageing /Differentiation
Tissue Engineering
Telomerase Gene Expression in Different MSC populations
INTRODUCTION
Mimic a scaffold by tissue decellularization and obtain one extracellular matrix (ECM) rich bioscaffold
Stem cells: Cells that are able to
(1) Self-renew (can create more stem cells indefinitely)
(2)  Differentiate into (become) specialized, mature cell types
CLASSIFICATION OF STEM CELLS
Plasticity
Totipotent
Multipotent
Pluripotent
Unipotent
Growth stage
Embryonic stem cell
Mesenchymal stem cells (MSCs)
Tissue specific-stem cells (adult stem cells)
Place of existence
Embryonic stem cells
ESCs are derived from fetal tissue
Stem cells derived from the umbilical cord
Adults stem cells as bone marrow.
Modified SURFACES
BIO SCAFFOLDS
BIOMIMETIC SCAFFOLD
TISSUE ENGINEERING starts with CELLS and a SCAFFOLD
DECELLURIZED HUMAN PANCREAS
Nanobiphasic ceramics with MSC for
BONE RECONSTRUCTION
DECELLURIZED KIDNEY
50:50
55:45
References
Thejaswi Kalagara, Amarnath Miriyala, Srinivas G, Mahesh Kumar Jerald, T Avinash Raj, S. Singh (2012) Immune modulatory responses of Mesenchymal stem cells from different sources in cultures and in vivo. Cell & Tissue Transplantation & Therapy 2012:4 1–13
S Reddy, S Wasnik, A Guha, JM Kumar, A Sinha, S Singh (2012) Evaluation of nano-biphasic calcium phosphate ceramics for bone tissue engineering applications: In vitro and preliminary in vivo studies Journal of Biomaterials Applications DOI: /  
S Reddy, M Amarnath, A Sinha, S Singh (2011) NanoBiphasic Ceramics for Tissue Engineering: A Review Journal of Biona
noscience 5 (1), 26-32
Sujatha, G.; Sinha, Arvind; Singh, Shashi (2011) Cells Behaviour in Presence of Nano-Scaffolds. Journal of Biomedical Nanotechnology, 7(1) 43-44

2. Center for Cellular and Molecular Biology, Hyderabad
Biology of Mesenchymal Stem Cells from various sources and their potential use in Tissue Engineering
Rekha Tripathi, Udayachandrika.K, M. Amarnath, Sujata Reddy, Dr. Shashi Singh
Center for Cellular and Molecular Biology, Hyderabad
CHARACTERIZATION
ABSTRACT
ABSTrACT
IMMUNE RESPONSE OF MSCS
We present a comparison of immune-properties of MSC isolated from PL, AD, WJ and BM.These study suggest that MSCs from PL,AD,WJ could be substituted for BMSCs.
Adult stem cells are maintained in various tissues in a quiescent state, which is believed to protect them from various stresses and transformations. The basic stem cell traits are defined as the cell’s capacity to divide indefinitely and plasticity - the ability to differentiate into many different cell types. Adult stem cells are being derived from various sources- placenta, Wharton’s jelly, cord blood, bone marrow, lipo-aspirate and characterized in our laboratory. All the sources yield a large number of highly potential mesenchymal stem cell that expand in cultures; have markers defined for MSC and differentiate into osteogenic, chondrogenic, neurogenic and endothelial lineages when induced. These MSC do not elicit any proliferative response to allogeneic lymphocytes in culture conditions; they do not release any cytokines that might support the proliferative activity and show immune suppressive effects when injected to immune-competent mice. The proliferation capacity of MSC differ slightly. MSC derived from placenta and Wharton’s jelly show longer survival in cultures up to 16 passages whereas the ones derived from bone marrow, cord blood or adipose rarely go beyond 6 passage and age rapidly. These cells are being used to study effects of niche modifications using nanostructures.
Immune suppression is induced by MSC derived from different sources both in vitro and in vivo
MESENCHYMAL STEM CELLS (MSCs)
AGEING OF MSC
STUDY OF DIFFERENTIATION
Growth curve of MSC from different sources
Characterization of MSC
Immune Response of MSC
Study of Ageing /Differentiation
Tissue Engineering
Telomerase Gene Expression in Different MSC populations
INTRODUCTION
Mimic a scaffold by tissue decellularization and obtain one extracellular matrix (ECM) rich bioscaffold
Stem cells: Cells that are able to
(1) Self-renew (can create more stem cells indefinitely)
(2)  Differentiate into (become) specialized, mature cell types
CLASSIFICATION OF STEM CELLS
Plasticity
Totipotent
Multipotent
Pluripotent
Unipotent
Growth stage
Embryonic stem cell
Mesenchymal stem cells (MSCs)
Tissue specific-stem cells (adult stem cells)
Place of existence
Embryonic stem cells
ESCs are derived from fetal tissue
Stem cells derived from the umbilical cord
Adults stem cells as bone marrow.
Modified SURFACES
BIO SCAFFOLDS
BIOMIMETIC SCAFFOLD
TISSUE ENGINEERING starts with CELLS and a SCAFFOLD
DECELLURIZED HUMAN PANCREAS
Nanobiphasic ceramics with MSC for
BONE RECONSTRUCTION
DECELLURIZED KIDNEY
50:50
55:45
References
Thejaswi Kalagara, Amarnath Miriyala, Srinivas G, Mahesh Kumar Jerald, T Avinash Raj, S. Singh (2012) Immune modulatory responses of Mesenchymal stem cells from different sources in cultures and in vivo. Cell & Tissue Transplantation & Therapy 2012:4 1–13 .
S Reddy, S Wasnik, A Guha, JM Kumar, A Sinha, S Singh (2012) Evaluation of nano-biphasic calcium phosphate ceramics for bone tissue engineering applications: In vitro and preliminary in vivo studies Journal of Biomaterials Applications . DOI: /
S Reddy, M Amarnath, A Sinha, S Singh (2011) NanoBiphasic Ceramics for Tissue Engineering: A Review Journal of Bionanoscience 5 (1), 26-32
Sujatha, G.; Sinha, Arvind; Singh, Shashi (2011) Cells Behaviour in Presence of Nano-Scaffolds. Journal of Biomedical Nanotechnology, 7(1) 43-44

3. Center for Cellular and Molecular Biology, Hyderabad
Biology of Mesenchymal Stem Cells from various sources and their potential use in Tissue Engineering
Rekha Tripathi, Udayachandrika.K, M. Amarnath, Sujata Reddy, Dr. Shashi Singh
Center for Cellular and Molecular Biology, Hyderabad
CHARACTERIZATION
ABSTRACT
ABSTrACT
IMMUNE RESPONSE OF MSCS
We present a comparison of immune-properties of MSC isolated from PL, AD, WJ and BM.These study suggest that MSCs from PL,AD,WJ could be substituted for BMSCs.
Adult stem cells are maintained in various tissues in a quiescent state, which is believed to protect them from various stresses and transformations. The basic stem cell traits are defined as the cell’s capacity to divide indefinitely and plasticity - the ability to differentiate into many different cell types. Adult stem cells are being derived from various sources- placenta, Wharton’s jelly, cord blood, bone marrow, lipo-aspirate and characterized in our laboratory. All the sources yield a large number of highly potential mesenchymal stem cell that expand in cultures; have markers defined for MSC and differentiate into osteogenic, chondrogenic, neurogenic and endothelial lineages when induced. These MSC do not elicit any proliferative response to allogeneic lymphocytes in culture conditions; they do not release any cytokines that might support the proliferative activity and show immune suppressive effects when injected to immune-competent mice. The proliferation capacity of MSC differ slightly. MSC derived from placenta and Wharton’s jelly show longer survival in cultures up to 16 passages whereas the ones derived from bone marrow, cord blood or adipose rarely go beyond 6 passage and age rapidly. These cells are being used to study effects of niche modifications using nanostructures.
Immune suppression is induced by MSC derived from different sources both in vitro and in vivo
MESENCHYMAL STEM CELLS (MSCs)
AGEING OF MSC
STUDY OF DIFFERENTIATION
Growth curve of MSC from different sources
Characterization of MSC
Immune Response of MSC
Study of Ageing /Differentiation
Tissue Engineering
Telomerase Gene Expression in Different MSC populations
INTRODUCTION
Mimic a scaffold by tissue decellularization and obtain one extracellular matrix (ECM) rich bioscaffold
Stem cells: Cells that are able to
(1) Self-renew (can create more stem cells indefinitely)
(2)  Differentiate into (become) specialized, mature cell types
CLASSIFICATION OF STEM CELLS
Plasticity
Totipotent
Multipotent
Pluripotent
Unipotent
Growth stage
Embryonic stem cell
Mesenchymal stem cells (MSCs)
Tissue specific-stem cells (adult stem cells)
Place of existence
Embryonic stem cells
ESCs are derived from fetal tissue
Stem cells derived from the umbilical cord
Adults stem cells as bone marrow.
Modified SURFACES
BIO SCAFFOLDS
BIOMIMETIC SCAFFOLD
TISSUE ENGINEERING starts with CELLS and a SCAFFOLD
DECELLURIZED HUMAN PANCREAS
Nanobiphasic ceramics with MSC for
BONE RECONSTRUCTION
DECELLURIZED KIDNEY
50:50
55:45
References
Thejaswi Kalagara, Amarnath Miriyala, Srinivas G, Mahesh Kumar Jerald, T Avinash Raj, S. Singh (2012) Immune modulatory responses of Mesenchymal stem cells from different sources in cultures and in vivo. Cell & Tissue Transplantation & Therapy 2012:4 1–13 .
S Reddy, S Wasnik, A Guha, JM Kumar, A Sinha, S Singh (2012) Evaluation of nano-biphasic calcium phosphate ceramics for bone tissue engineering applications: In vitro and preliminary in vivo studies Journal of Biomaterials Applications . DOI: /
S Reddy, M Amarnath, A Sinha, S Singh (2011) NanoBiphasic Ceramics for Tissue Engineering: A Review Journal of Bionanoscience 5 (1), 26-32
Sujatha, G.; Sinha, Arvind; Singh, Shashi (2011) Cells Behaviour in Presence of Nano-Scaffolds. Journal of Biomedical Nanotechnology, 7(1) 43-44