1. BIOSYNTHESIS OF SILVER NANOPARTICLES USING MELIA DUBIA LEAF AQUEOUS EXTRACT AND ITS ANTIBACTERIAL ACTIVITY  
Submitted by: Under the Guidence of
G.Dayana Jeyaleela Dr.J.Rosaline Vimala
Ph.D Research scholar, Assistant professor
PG & Research Department of chemistry, PG & Research Department of chemistry,
Holy cross college,trichy Holy cross college,trichy-1

2. INTRODUCTION:
Synthesis of nano particles can be performed using a number of
chemical and physical methods.
synthesis of nanoparticles using green synthesis method.
APPLICATIONS:
pharmaceutical, electronics,food and feed, biomedical science, drug and gene delivery, chemical industry,cosmetics, mechanics space industries and treatments of infection cancer, allergy, diabetes and inflammation.
AIM OF THE WORK:
To synthesize the silver nanoparticles by using a plant leaf extract of
Melia dubia
biosynthesized AgNPs were characterized by using UV-Vis analysis, FTIR,
XRD and SEM analysis
To study the Antimicrobial activity of the silver bio-nanoparticles

3. MATERIALS AND METHODS:
Preparation of Melia dubia leaf extract:
collection of plant -leaves were dried under sunshade -powdered -plant powder and 100 ml of distilled water - boil at 60°C for 30 min then filtered through Whatman No.1 filter paper
Synthesis of AgNPs:
Aqueous solution (3 mM) of silver nitrate was prepared-25 ml solution & leaf extract was added -mixture was then kept on magnetic stirrer-colour change -time and colour change were recorded .
Characterization Techniques:
The formation of silver natenoparticles confirmed by following techniques UV-Vis , FTIR,SEM,XRD
Anti Bactirial Activity:
disc diffusion method

4. Profile of the Melia dubia Melia dubia flowers & leaves
Family: Meliaceae
Genus: Melia
Species: M. dubia
Tamil : malai veembu
Uses of M.dubia
Synthesis of silver nanoparticles using leaves of melia dubia

5. RESULTS AND DISCUSSION:
UV-Visible Spectral Analysis:
UV-Vis spectra were taken at different time period for 3mm AgNO3 solution (30mins to 6hrs). The spectra showed the peak approximately at nm, clearly indicating the formation of spherical AgNPs in the aqueous extract of Meliadubia.
Figure- 1: UV- Visible spectra were taken at different time period
for 3mm AgNO3 solution (30mins to 6hrs)

6. FTIR analysis:
FTIR graph of AgNO3
FTIR graph of Leaf extract of Melia dubia
FTIR graph of Synthesized AGNPs

7. OH stretching cm-1
C=O stretching cm-1
N-H stretching cm-1
C-Cl stretching cm-1
C=N stretching cm-1
CH stretching cm-1
N-O stretching
Other details
Silver nitrate
3467,1357.7,653.61
Plant extract
670.34
, ,
AgNPs
676.23
for CH2 & CH3 deformation
plant extract shows broad peak at cm-1 - presence of OH group or carboxyl groups - after synthesis of AgNPs –shift at cm-1 indicating the NH stretching - confirming the biofabrication of the nanoparticles - action of the phytochemicals

8. XRD pattern of Meliadubia
SEM image of Meliadubia
SEM analyze the shape of the silver nano particles - silver nano particles - spherical in shape – XRD analyze phasedistribution, crystallinity,purity of the synthesised nanoparticles particles.
crystalline approximate spherical shape with size equivalent to 21.7nm with no impurities.

9. Aqueous Extract added and Zone of inhibition (mm/ml)
Antibacterial Activity:
PATHOGENS
Aqueous Extract added and Zone of inhibition (mm/ml)
25 µl
50 µl
75 µl
100 µl
Control
Staphylococcus aureus 15 17 18 20
E.coli 22
Klebsiellapneumoniae 12 21 28
Vibrio cholera 25
CONCLUSION:
AgNPs successfully obtained from bioreduction of silver nitrate solutions using Meliadubia leaf extract AgNPs characterized using UV-vis ,IR, SEM and XRD analysis
Plant extract act as a reducing agent( Ag+ to Ag).
The particles also exhibited good antimicrobial activity against K. pneumonia, S.aureus, V. choleraeand E.coli.Hence, due to their stable nature and antimicrobial property, these AgNPs may be well utilized in industrial and remedial purposes

10. THANK YOU