1. Sterilisation Of Surgical Instrument

2. It is essential to clean instrument as posible after use:
To avoid rusting
To remove soil before it can dry and harden in serration,crevices etc
Washing proces should remove all particle of adhering tissue,dried blood,or accumulation of salt in serration of instrument
Hand brush may be used for cleaning purpose
The instrument should be free from oil and grease

3. Inaccessible soil or garbage can be removed by soking of instrument in water at 52c to which detergent can be added
Immediately after washing, the instrument are rinsed in boiling water and then dried thoroughly while still hot
If any moisture remain in instrument,they may rust on storage
Lubrication or oiling of instrument should be avoided before sterilisation
Presence of film of oil on instrument serve as protective barrier for organisms

4. Preparing instrument for steralisation:
Place a layer of soft fabric in the bottom of the tray, then lay the instrument on this cloth
The tray must be provided with perforated or wire mesh bottom
A wire mesh is necessary for effective sterilisation
All joint instrument must be open

5. Sterilisation cycle:
Steam sterilisation of surgical instrument
Instrument are sterilisation for 15 min at 121c or for 7 min at 132c in high speed ,pressure,instrument steriliser
After sterilisation,trays of instrument allowed to remain in steriliser with door slightly open for min to ensure drying

6. Infra-red sterilization in high vaccum In this type,short exposure of 3 min to a high temp 280c at vaccum of 1-2 mm of Hg is given
Emergency sterilisation of instrument
In this type, there should not be any compromise on safety
Method selected must be adequate for destruction of resistance spore

9. Reference Hospital pharmacy -tipnis and bajaj