1. Parts of the
Microscope

2. Compound Light Microscope
Parts and Functions

3. Compound Light Microscope
Parts and Functions
eyepiece

4. Eyepiece – what you look through, where you place your eye

5. Compound Light Microscope
Parts and Functions
eyepiece
arm

6. Eyepiece – what you look through, where you place your eye
Arm – supports the body tube and lenses

7. Compound Light Microscope
Parts and Functions
eyepiece
arm
stage

8. Eyepiece – what you look through, where you place your eye
Arm – supports the body tube and lenses
Stage – the platform on which a slide is placed

9. Compound Light Microscope
Parts and Functions
eyepiece
arm
stage
coarse focus
adjustment

10. Eyepiece – what you look through, where you place your eye
Arm – supports the body tube and lenses
Stage – the platform on which a slide is placed
Coarse focus adjustment – knob that makes large adjustments to
the focus

11. Compound Light Microscope
Parts and Functions
eyepiece
arm
stage
coarse focus
adjustment
fine focus
adjustment

12. Eyepiece – what you look through, where you place your eye
Arm – supports the body tube and lenses
Stage – the platform on which a slide is placed
Coarse focus adjustment – knob that makes large adjustments to
the focus
Fine focus adjustment – knob that makes small adjustments
to the focus

13. Compound Light Microscope
Parts and Functions
eyepiece
arm
stage
coarse focus
adjustment
fine focus
adjustment
base

14. Eyepiece – what you look through, where you place your eye
Arm – supports the body tube and lenses
Stage – the platform on which a slide is placed
Coarse focus adjustment – knob that makes large adjustments to
the focus
Fine focus adjustment – knob that makes small adjustments
to the focus
Base – supports the microscope

15. Compound Light Microscope
Parts and Functions
eyepiece
arm
stage
coarse focus
adjustment
light source
fine focus
adjustment
base

16. Eyepiece – what you look through, where you place your eye
Arm – supports the body tube and lenses
Stage – the platform on which a slide is placed
Coarse focus adjustment – knob that makes large adjustments to
the focus
Fine focus adjustment – knob that makes small adjustments
to the focus
Base – supports the microscope
Light source – directs light upwards onto the slide

17. Compound Light Microscope
Parts and Functions
eyepiece
arm
stage
coarse focus
adjustment
diaphragm
light source
fine focus
adjustment
base

18. Eyepiece – what you look through, where you place your eye
Arm – supports the body tube and lenses
Stage – the platform on which a slide is placed
Coarse focus adjustment – knob that makes large adjustments to
the focus
Fine focus adjustment – knob that makes small adjustments
to the focus
Base – supports the microscope
Light source – directs light upwards onto the slide
Diaphragm – adjustable opening under stage, allows different
amounts of light

19. Compound Light Microscope
Parts and Functions
eyepiece
arm
stage
stage clips
coarse focus
adjustment
diaphragm
light source
fine focus
adjustment
base

20. Eyepiece – what you look through, where you place your eye
Arm – supports the body tube and lenses
Stage – the platform on which a slide is placed
Coarse focus adjustment – knob that makes large adjustments to
the focus
Fine focus adjustment – knob that makes small adjustments
to the focus
Base – supports the microscope
Light source – directs light upwards onto the slide
Diaphragm – adjustable opening under stage, allows different
amounts of light
Stage clips – holds the slide securely onto the stage

21. Compound Light Microscope
Parts and Functions
eyepiece
arm
objective lenses
stage
stage clips
coarse focus
adjustment
diaphragm
light source
fine focus
adjustment
base

22. Eyepiece – what you look through, where you place your eye
Arm – supports the body tube and lenses
Stage – the platform on which a slide is placed
Coarse focus adjustment – knob that makes large adjustments to
the focus
Fine focus adjustment – knob that makes small adjustments
to the focus
Base – supports the microscope
Light source – directs light upwards onto the slide
Diaphragm – adjustable opening under stage, allows different
amounts of light
Stage clips – holds the slide securely onto the stage
Low Power Objective – a small lens with low magnifying power
High Power Objective – a large lens with high magnifying power

23. Compound Light Microscope
Parts and Functions
eyepiece
revolving
nosepiece
arm
objective lenses
stage
stage clips
coarse focus
adjustment
diaphragm
light source
fine focus
adjustment
base

24. Eyepiece – what you look through, where you place your eye
Arm – supports the body tube and lenses
Stage – the platform on which a slide is placed
Coarse focus adjustment – knob that makes large adjustments to
the focus
Fine focus adjustment – knob that makes small adjustments
to the focus
Base – supports the microscope
Light source – directs light upwards onto the slide
Diaphragm – adjustable opening under stage, allows different
amounts of light
Stage clips – holds the slide securely onto the stage
Low Power Objective – a small lens with low magnifying power
High Power Objective – a large lens with high magnifying power
Revolving Nosepiece – rotating device that holds the
objectives (lenses)

25. Compound Light Microscope
Parts and Functions
eyepiece
body tube
revolving
nosepiece
arm
objective lenses
stage
stage clips
coarse focus
adjustment
diaphragm
light source
fine focus
adjustment
base

26. Eyepiece – what you look through, where you place your eye
Arm – supports the body tube and lenses
Stage – the platform on which a slide is placed
Coarse focus adjustment – knob that makes large adjustments to
the focus
Fine focus adjustment – knob that makes small adjustments
to the focus
Base – supports the microscope
Light source – directs light upwards onto the slide
Diaphragm – adjustable opening under stage, allows different
amounts of light
Stage clips – holds the slide securely onto the stage
Low Power Objective – a small lens with low magnifying power
High Power Objective – a large lens with high magnifying power
Revolving Nosepiece – rotating device that holds the
objectives (lenses)
Body tube – tube that supports the eyepiece

27. Handling the Microscope
 Carry the microscope with two hands –
one under base and the other on the arm
 Place the microscope on the table with the
arm facing towards you
Place the microscope at least 10 cm from the
edge of the table
Always wipe the lenses with lens paper before
and after use

28. Focusing the Microscope
 Look into eyepiece with both eyes open
 Always focus first with the low power objective
using the coarse adjustment
 Use the fine adjustment to make the image
clearer and sharper
 Never use the coarse adjustment knob while
using high power
 Be careful that the objective lenses do not touch
the glass slide
 Never search for the specimen under high power

29. Storage of the Microscope
 Store microscope with low power objective
in place, with stage racked all the way down,
cord wrapped up, and with the cover on the
microscope

30. Determining Total Magnification
Magnification = the ability to make an image appear
larger compared to its actual size
The formula for determining total magnification is:
Total magnification = ocular magnification x objective magnification

31. Determining Total Magnification
The formula for determining total magnification is:
Total magnification = ocular magnification x objective magnification
Type of Lens
Magnification
Ocular
10x
Low power
Med power
High power

32. Determining Total Magnification
The formula for determining total magnification is:
Total magnification = ocular magnification x objective magnification
Type of Lens
Magnification
Ocular
10x
Low power 4x
Med power
High power

33. Determining Total Magnification
The formula for determining total magnification is:
Total magnification = ocular magnification x objective magnification
Type of Lens
Magnification
Ocular
10x
Low power 4x
Med power
High power

34. Determining Total Magnification
The formula for determining total magnification is:
Total magnification = ocular magnification x objective magnification
Type of Lens
Magnification
Ocular
10x
Low power 4x
Med power
High power
40x
What is the total magnification using the 4x objective lens?
10x x 4x = 40x
What is the total magnification using the 10x objective lens?
10x x 10x = 100x
What is the total magnification using the 40x objective lens?
10x x 40x = 400x