1. An Introduction
Microscopy

2. Microscopy
1b. Given the appropriate supplies, and equipment, apply the basic principles of microscopy to properly focus a microscope with no more than three instructor assists. (1) Components of a Compound Microscope (2) Types of Microscopes (3) Care of the Microscope (4) Operation (5) ORM Group Discussion

3. Oculars Two that magnify the image 5x or 10x
May contain ocular micrometer
Microscopic ruler used to measure objects
Calibrated using a stage micrometer etched into a glass slide

4. Objectives Main magnifiers that capture primary image
Located on spring mounted revolving nosepiece
Named depending on power and function

5. Scanning 4x/5x power Not used in hemotology
Objectives
Scanning 4x/5x power Not used in hemotology

6. For initial focusing and some cell counts
Objectives
Low power 10x power
For initial focusing and some cell counts

7. High dry 40x to 45x power Used for some cell counts
Objectives
High dry 40x to 45x power Used for some cell counts

8. Oil immersion Objectives 100x power (may have a black ring)
Special immersion oil is used between slide and objective
Better resolving power at higher magnification
Used with fixed and stained slides

9. Body Contains lens system Oculars at top Objectives at bottom
Interpupillary distance control
Adjusts distance between oculars
Allows viewer to see one field of view

10. Arm Connects the base to body Holds stage and its components
Used to carry the microscope
Houses the coarse and fine focus knobs
Coarse – used to locate initial image
Fine – used to obtain optimum resolution

11. Stage
Movable platform used to hold slide and bring item of interest into field of view
Two control knobs
Side-to-side movement
Front-to-back movement
Stage clips hold slide in place
Light condenser attached to the underside of the stage

12. Base
Contains the transformer which converts electrical power from AC to DC to provide power to illuminator
Houses illuminator (light source)
Control switch is located on the base
On/off style
Variable intensity

13. Condenser Converges (bends) light into a beam by use of a convex lens
Condenser control knob moves it up and down

14. Iris diaphragm Attached to underside of condenser
Multi-leafed circular opening used to control the amount of light transmitted to condenser
Adjusted by lever or dial on condenser

15. Types of microscopes Phase Contrast Epifluorescence Electron Darkfield
Polarized

16. Care of Microscope Storage Dust and lint Oculars and objectives
Mirror/Illuminator/Condenser

17. Operation Plug in and turn on Lower stage to lowest position
Place slide into stage clip
Place on low power objective (10x)
Open iris diaphragm half-way
Place condenser in lowest position
Focus using coarse focus knob
Adjust light and fine focus
Adjust interpupillary distance

18. Operation
Rotate nosepiece until high dry objective clicks in to place and focus using fine focus
Switch between high dry and oil immersion objective
Place immersion oil on slide
Rotate the nosepiece until oil immersion objective clicks into place
Use fine focus knob to adjust to optimum resolution

19. Microscopy
1b. Given the appropriate supplies, and equipment, apply the basic principles of microscopy to properly focus a microscope with no more than three instructor assists. (1) Components of a Compound Microscope (2) Types of Microscopes (3) Care of the Microscope (4) Operation (5) ORM Group Discussion
This project was funded at $3,000,000 (100% of its total cost) from a grant awarded under the Trade Adjustment Assistance Community College and Career Training Grants, as implemented by the U.S. Department of Labor’s Employment and Training Administration. Rogue Community College is an equal opportunity employer/program. Auxiliary aids and services, alternate form and language services are available to individuals with disabilities and limited English proficiency free of cost upon request.
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