1. Biologists Tools
Using the microscope

2. Why these tools are important
New forms of imaging technology provides new views of life
It wasn’t until the invention of the microscope that people were able to see the world in a way that was smaller than what the eye could see.
Microscopes can come in one of two types, light or electron.

3. Light microscopes
Is a combination of lenses that use a light source to see an organism/object.
Many times this is combined with staining techniques to see the organism more clearly

7. e
Step 1:
Obtain clean slide and apply object to view.
Step 2:
Add one drop of H2O to object in center of slide e
Step 3:
Apply plastic cover slip to top of H2O and object to view. e

8. Paper Towel
Step 4:
Draw up any extra H2O from the edge of the cover slip using the corner of a paper towel e
Note:
When applying coverslip, do so at a 45 degree angle and lower onto surface. (Stops so many oxygen bubbles from forming)

9. Electron microscopes
Uses beams of electrons instead of light to scan objects.
Much more precise that light microscopes
100,000 x magnification
Can’t be used to study living organisms
Has to be held in a vacuum

10. Electron Microscopes Two main types:
Transmission electron microscope (TEM)
Looks at interior of organism
Can be the highest magnification
Scanning electron microscope (SEM)
Scans surface of an organism
Usually cover the organism in metal (usually gold).

19. Medical Imaging X-Ray Magnetic resonance imaging (MRI)
X-rays are passed through an tissues and is absorbed by bones and teeth.
Magnetic resonance imaging (MRI)
Can be used on ligaments, muscles, and tissue
Can observe organs as well

22. Computers
Are usually used to analyze and process the images received from advanced microscopes
Computers are also used to model complex systems