1. Presenting author: maheshmg2012@gmail.com, *yash28bhandary@gmail.com
Expression levels of Inflammatory cytokines and p53-fibrinolytic system in smokers with or without COPD
Mahesh MG1, Deepu C2, Irfan KM2, Ashwini S3, Bhandary YP1*
Yenepoya Research Centre, Yenepoya University, Deralakatte, Mangalore
Presenting author:
Results
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INTRODUCTION
RESULTS
Samples
Control
Smokers with COPD
Smokers without COPD
Blood 3 9 8
Serum
Sputum
Chronic obstructive pulmonary disease (COPD) is a disease characterized by inflammation in airway and obstruction of lung function.
COPD patient are more often found with alveolar inflammation due to accumulation of inflammatory cells, elevated pulmonary IL-17A.
Defective fibrinolysis and alveolar fibrin deposition due to a disproportionate increase in plasminogen activator inhibitor (PAI)-1 and epithelial cell apoptosis.
Figure 1: IL-17A expression levels in normal and COPD patient. Blood samples were collected from normal and COPD patients. The serum samples were subjected to western blotting analysis for IL-17A using IL-17 A antibody. Samples were normalized with β-actin antibody.
Table-1: Collection of Blood and sputum samples collected.
Parameters
Smokers with COPD
Stage I
Stage II
Stage III
Stage IV
No of Patients 9 4 3 2 -
Average Age
40-85
40-60
55-85
50-70
Sex M
Smokers without COPD 8
30-70
Normal control
No.
40-65
Figure 2: p53 and P-p53 expression levels in normal and COPD patient. Blood samples were collected from normal and COPD patients. The serum samples were subjected to western blotting analysis for p53, and PAI-1 expression levels using their respective antibody. Samples were normalized with β -actin antibody.
Table-3: Categorization of COPD samples based on FEV1/FVC ratio.
WORK FLOW
Normal
Smokers with COPD
Smokers without COPD
Stage I
Stage II
Stage III
Stage IV
 Eosinophils
 Hb
 MCH
 Lymphocytes
Lymphocytes
 PCV -
Collection of blood samples
Biochemical analysis
Screening for IL-17A expression
Screening for p53-fibrinolytic system expression
Figure 3: Cleaved and total caspase-3 levels in normal and COPD patient. Blood samples were collected from normal and COPD patients. The serum samples were subjected to western blotting analysis for cl.caspase-3 and t.caspase-3 levels using their respective antibody. Samples were normalized with β-actin antibody
Table-2: Biochemical analysis of COPD samples.
DISCUSSION
No major changes in the biochemical analysis seen among smokers with or without COPD
Increased expression of IL-17A and p53-fibrinolytic system was observed in smokers with COPD
The elevated expression of IL-17A plays a major role in p53 expression and its regulation
CONCLUSION
A novel approach to prove the IL-17A mediated p53-fibrinolytic system during COPD. It may be useful to find out a novel pathway for the better understanding of molecular mechanism involved in COPD.
ACKNOWLEDGEMENT
The authors acknowledge Yenepoya Research Centre, and Department of pulmonary medicine, Yenepoya Medical college, Yenepoya University for the support