1. Supplementary Figure S4
8 h
control
TGF-b1
10 ng/ml
PAI-1 mRNA / TBP 10 20 30
Control siRNA
CTGF siRNA
*** *
n.s.
Figure S4.
Connective tissue growth factor short-interfering RNA (CTGF siRNA) suppressed plasminogen activator inhibitor-1 (PAI-1) messenger RNA (mRNA) expression in cultured human renal proximal tubular epithelial cells (HK-2) treated with transforming growth factor-b1 (TGF-b1).
HK-2 cells were transfected with CTGF siRNA and treated with TGF-b1. Non-targeting siRNA was used as a control siRNA. PAI-1 mRNA expression was determined by quantitative real-time PCR. TATA-box binding protein (TBP) was used as an internal control. PAI-1 mRNA expression was significantly increased by TGF-b1 treatment after 8 h incubation. TGF-b1-induced PAI-1 mRNA upregulation was significantly suppressed by CTGF siRNA. Data are means ± s.e. (N = 4). *P < 0.05, ***P < 0.001, n.s.: not significant.
Supplementary Figure S4