1. DNA and DNA Replication

3. Discovery of DNA an ACCIDENT
Friedrich Miescher was working with white blood cells.
He began gathering pus from the bandages of patients from a local hospital.
After using a salt solution to wash the pus from the bandages, and using an alkaline solution, the nucleus from those white blood cells were obtained.

5. DNA (Deoxyribonucleic Acid)
Why is DNA called the “Blue Print of Life”?
It has all the instructions the cell needs to make protein, organelles, carry out chemical reactions and to survive

6. DNA – double helix shape
Like a spiral staircase.

7. What is the backbone of DNA?
Phosphate and sugar

8. Watson and Crick Famous photograph of two young men given
credit for discovering
the shape of the DNA
molecule as a double
helix.
BUT!

9. Here are the actual facts!

10. Rosaline Franklin – knew the shape of DNA before others.

12. Base Pair Rule in DNA

13. 1. Name the bond
between the
Nitrogen bases.
If the DNA unzips
where will it
unzip at?

14. The Building Block or monomer of Nucleic Acids
Nucleotide

15. DNA Template (pattern)

16. DNA in Prokaryote and Eukaryote Cells

17. Real photograph of bacterial (Prokaryote) DNA

18. Bacterial DNA looked at with TEM 1,000,000 X

19. DNA in Eukaryote Cell

20. Eukaryote Cell

21. What are function of Histones?

22. Chromatin into Chromosome

25. DNA
Replication
is the biological process of producing two identical copies of DNA from one original DNA molecule.

26. Why does DNA need to replicate?
So when the cell divides (mitosis) each
new cell will have a complete copy of
instructions (DNA).
2. First step in protein synthesis. (when DNA
unzips so mRNA can get code)
3. DNA replication occurs in all living organisms
and is the basis for biological inheritance.

27. When does DNA replication occur?
1. During what phase of the
Cell cycle does DNA
Replication occur?
Does DNA replication
happen (during or before)
mitosis?

28. DNA Replication (copying itself)

29. Explain the following Cartoon!

30. Enzymes involved in DNA replication?
Replication requires enzymes:
1) Helicase – unwinds DNA
2) DNA polymerase – puts new
nucleotides in place & proofreads

32. The process of DNA replication
DNA unwinds & unzips, using Helicase
Replication fork forms, (area where 2 strands separate)
* There can be several replication forks at the same time.
3) DNA polymerase adds new nucleotides to each DNA strand!

33. Biotechnology

34. DNA Replication in vitro (artificially, outside a cell).
The process is called PCR (polymerase chain reaction) and is used in molecular biology to amplify a single copy of DNA into millions of copies of the same DNA sequence.

35. PCR Machine Technology developed for Biotechnology!
(Polymerase Chain Reaction)
Technology
developed for
Biotechnology!
(DNA replication out
side of a cell)

36. (DNA Coping itself)

37. PCR Process using Blood