1. National Conference on Indigenous Systems of Medicine
Antiglycation, antioxidant potentials and total phenolic content of Scoparia dulcis decoction
Rupika Perera, S Ekanayake and K K D S Ranaweera
University of Sri Jayewardenepura
National Conference on Indigenous Systems of Medicine
2015

2. Diabetes mellitus
Global prevalence of diabetes mellitus is rising and it has become the most challenging health problem in the 21st century.
285 million among the seven billion of the total world population suffer from diabetes mellitus.
> 10% of the Sri Lankan population currently has diabetes and 36% of all diabetic subjects are undiagnosed.
International Diabetes Federation, 4th Edition,2009 ISBN – 13:

3. Diabetic complications
Long term uncontrolled diabetes mellitus leads to several complications
Retinopathy
Neuropathy
Nephropathy
Embriopathy
Cardiovascular diseases

4. Glycation is a key molecular basis of diabetic complications due to hyperglycemia
HbA1c - glycated hemoglobin is taken as a measure of the average blood glucose level in diabetic patients for a period of time

5. Glycation
Non enzymatic reaction between carbonyl group of reducing sugars and free amino group of biological proteins result in the formation of glycated proteins.
Early Intermediate Late

6. Non Enzymic Glycation Reaction
hours
days
weeks - months
+ Free radicals

7. Oxidation process and free radical generation in glycation process
Amadori products Glucose
Intermediate carbonyl compounds
Protein crosslink and AGEs
Excessive amounts of free radicals generate during the glycation process.
Metal ion catalyzed auto oxidation
oxidation
Agents with anti oxidative and metal -chelating properties retard the formation of AGEs.
Lysine/arginine residue

8. Medicinal Plants & Diabetes
Medicinal plants with antiglycation and antioxidant properties are good therapeutic potentials to prevent diabetic complications.
Increased global interest in traditional medicine.
Pharmaceutical companies have renewed their strategies in favour of natural product drug development and discovery.

9. Scoparia dulcis
Scoparia dulcis is a perennial herb (sweet broom weed) and the decoction prepared using the tender parts of the plant is used as a traditional treatment for diabetes mellitus.
Few experiments have been carried out to prove the scientific efficacy of using this plant in diabetes mellitus.

10. Objective
To determine the antiglycation and antioxidant potentials and total phenolic content in the decoction of Scoparia dulcis used as a traditional treatment for diabetes mellitus.

11. Methodology

12. Collection and preparation of samples
commercially available .
Fresh samples Commercial samples
S.dulcis Bandarawela,
plants Wijerama
(Fresh ) Rukmale
Traditional herbal market Colombo
S dulcis
(commercial)
Air dried for 24 hrs
Air dried for 24 hrs
Dried at 55 ̊C in a dehydrator
Corse powder
Corse Powder

13. Preparation of water extract (decoction)
Powdered plant material
Simmerly boiled with distilled water
(60g sample / 960 mL of water until final
volume is 240 mL)
Filtered through a silk cloth
Stored at -4ºc in air tight container
Preparation of water extract (decoction)

14. Analysis of S.dulcis decoction
ABTS method
Antioxidant activity
DPPH method
Antiglycation activity
Bovian Serum Albumin assay (BSA assay)
Total phenolic content
Folin Ciocalteu test

15. Total Phenolic content
Methodology
DPPH method
ABTS method
BSA assay
Total Phenolic content

16. Results and Discussion

17. Antioxidant activities of S.dulcis decoction
commercial sample showed high (615 TEAC mmol/g) compared to the laboratory dried samples but are not significantly different from each other ( TEAC mmol/g).
commercial sample and samples dried under laboratory conditions were in the range of 450 – 540 µg/ml, BHT 20 µg/ml.

18. Antiglycation activity and Total phenolic content of S dulcis decoction
Antiglycation activity is inversely proportional to the sample concentration. Bandarawela sample showed higher activity followed by com, Rukmale and Wijerama.
The highest TP content was found in Wijerama sample followed by Rukmale , Bandarawela and com.

19. Conclusion
Data from the study prove the antidiabetic properties of Scoparia dulcis decoction and the efficacy of using it as a traditional medicine in the treatment of diabetes mellitus and the plant is a promising source and preventive agent of protein glycation.

20. Acknowledgement
Gratefully acknowledge the financial support by the University Grant (Grant No. ASP/08/RE/2008/09) of University of Sri Jayewardenepura.
Our sincere thanks to :
Department of Food Science and Technology of University of Sri Jayewardenepura
Herbal Technology Division of Industrial Technology Institute (ITI)
Bandaranayke Memorial Ayurvedha Research Institute (BMARI)