Presentation is loading. Please wait.

Presentation is loading. Please wait.

Relationship between Genotype and Phenotype

Similar presentations


Presentation on theme: "Relationship between Genotype and Phenotype"— Presentation transcript:

1 Relationship between Genotype and Phenotype
Molecular Basis for Relationship between Genotype and Phenotype genotype DNA DNA sequence transcription RNA translation amino acid sequence protein function phenotype organism

2 Transgenesis Introduction of foreign genetic material into a host organism. 2. Ability of the new genetic material to be transmitted to offspring of the host. 3. Potential of the new genetic material to be expressed in the host. 4. Transgenic animals or transgenic plants are the result of this process.

3 Ti (tumor-inducing) plasmid is 200-kb double-stranded circular DNA.
Agrobacterium tumefaciens (soil bacterium) causes crown gall disease in infected plants. Ti (tumor-inducing) plasmid is 200-kb double-stranded circular DNA. T-DNA region is transferred and randomly inserted into host plant genome. T-DNA directs tumor production and synthesis of opines, compounds that the bacterium needs for growth.

4 Genetic Engineering in Plants using Ti Plasmid
T-DNA can be replaced by DNA sequence of interest and introduced into host plant genome. Reporter genes (selectable markers) are spliced into the recombinant T-DNA. Successful integration is indicated by expression of reporter genes. Markers (reporter genes) such as kanamycin resistance gene are very useful in monitoring successful transformation.

5 Generation of a Transgenic Plant

6 Genetic Engineering in Plants
Plants that have been transformed include horse-radish, morning glory, cucumber, carrot, yam, cotton, sunflower, lettuce, tomato, corn, soybean, alfalfa, tobacco, potato, and clover. Agriculturally important crops can be engineered to express: - viral resistance - pesticide resistance - herbicide resistance - insect resistance Nutritional value can be enhanced, for example, by introduction of genes that increase seed protein content. Plants can be engineered to produce pharmaceutically important proteins or vaccines. Luciferase gene is useful as a reporter gene for transformation.

7 Germ-line Therapy vs Somatic Therapy
Gene Therapy Germ-line Therapy vs Somatic Therapy

8 Gene Therapy in Mammals

9 Approaches to treatment of diseases
Human Gene Therapy Approaches to treatment of diseases DNA transfer into afflicted individual

10 Severe Combined Immunodeficiency
1990 : First Clinical Trial Severe Combined Immunodeficiency (SCID) - defective adenosine deaminase (ADA) gene. - afflicted patients express no functional T or B lymphocytes. - “Bubble Boy Disease”

11 Delivery Approaches to Somatic Therapy
Direct Administration: Introduction of normal gene using a virus retrovirus adenovirus Indirect Administration: Reintroduction of cells that have been made transgenic

12 Criteria for Gene Therapy: Candidacy of a Disease for Consideration
1. Mendelian inheritance 2. One gene product for the gene 3. Knowledge of function of gene product 4. Availability of normal gene sequence 5. Localized symptoms for the disease 6. Availability of delivery system

13 ~ 25,000 genes in humans are potential candidates for gene therapy.

14 Candidates for Gene Therapy ?

15 ~ 25,000 genes in humans are potential candidates for gene therapy.

16 Disease is usually fatal. Lungs, other organs are clogged with mucus.
Cystic Fibrosis Disease is usually fatal. Lungs, other organs are clogged with mucus. repeated bouts of pneumonia heart stressed by decreased lung capacity delivery failure of pancreatic enzymes Life expectancy of years ~1 of 29 Caucasians are carriers ~1 of 1600 Caucasians are affected ~30,000 Americans are affected

17 Cystic Fibrosis Caused by recessive mutation (chromosome 7)
Failure to pump Cl- ions across cell membrane Missing Cl- ion channel protein (cystic fibrosis transmembrane protein or CFTR) Normal gene sequence available (cloned in 1989) Normal gene 250 kb in size Transcript of 6 kb in size Gene product of 1480 amino acids in length Over 1500 mutations documented

18 Cystic Fibrosis as Candidate for Gene Therapy
1. Recessive gene on chromosome 7. 2. CFTR is the gene product. 3. CFTR is an ion channel protein. 4. Normal gene sequence has been cloned. 5. Symptoms are localized to specific organs. 6. Adenovirus (cold virus) can be used to deliver normal sequence to lung cells.

19 Human DNA Information: Who should have access to your DNA information?
Ethical Concerns Who should have access to your DNA information?


Download ppt "Relationship between Genotype and Phenotype"

Similar presentations


Ads by Google