1. OBJECTIVES a. To evaluate the impact of continuing schemes
b. To evaluate the quality as per Designated Best uses (DBU)
c. To establish nutrient budget and cycling of nutrients
d. To evaluate the impact of activities of catchment on the quality
e. To evaluate the changes in flora and fauna (defining biodiversity)

2. OUR APPROACH: SITE SELECTION
Site selected will be recorded with the help of GPS device.
Length of RS Lake will be divided into minimum 20 stations, distance and linear transect method will be used for sampling.
Appropriate statistical design (ANOVA/DUNCAN) will be followed depending on matching the questions being asked by the collection of data. Minimum number of sampling replicates will be estimated statistically.
Lake depth with meter stick, flow velocity with tennis ball and water temperature of the study site for each type of substratum with digital temperature probe.

3. BIOMONITORING
Biological monitoring, or biomonitoring, is the use of biological responses to assess changes in the environment, generally changes due to anthropogenic causes. Biomonitoring programmes may be qualitative, semi-quantitative, or quantitative.

4. BIOMONITORING: BENEFITS
Biological Monitoring will enable the Consultant to identify the following factors :
Identification of Pollutants/toxicants
Source of Pollutants/toxicants/anthropogenic fallouts, agricultural runoffs containing harmful agrochemicals, pesticides
Seasonal variations of the pollutants, toxicants etc.
Ecological changes
Species richness, variations, vulnerability of key species
Identification of Species sensitive to pollutants and tolerant species
Identification of Bioindicators
Number of macroinvertebrate taxa (e.g. family, genus) at site
Toxicity studies will enable to understand the level and presence of hazardous chemicals, toxicants, pollutants, etc.
Role of pollutants/toxicants may cause numbers or biomass to increase (for example, nutrients) or decrease (for example, pesticide).
Community level biomonitoring provides information on the magnitude and ecological effects of the stresses on the system.

5. Sensitive phytoplankton/benthos/nekton species of lakes
Bar H= -

6. CALCULATION OF VOLUME OF PHYTOPLANKTON
VOLUME INDICATOR OF
SENSITIVE PLANKTON

7. CALCULATION OF VOLUME OF PHYTOPLANKTON

8. SEARCH FOR VULNERABLE SPECIES: A NEW APPROACH
Development of a library for both endangered, key vulnerable floral
and faunal community and bioindicator species in the of Rabindra Saravar lake using DNA Barcoding.

9. The following parameters will be measured in aquatic system:pH
Temperature
Total Dissolved Solids
Total Suspended Solids
Conductivity
Turbidity
Colour
Odour
Oil & grease
Total Alkalinity
Total Hardness
Calcium
Magnesium
Dissolved Oxygen
Sulphates
Chloride
Carbonates and Bicarbonates
Sodium
Potassium
Mercury
Chromium
Cadmium
Lead
Nickel
Arsenic
Boron
Nitrate-Nitrogen
Ammoniacal Nitrogen
Total Nitrogen
Sulphates
Phosphates
BOD
COD
Total Pesticides

10. Phosphorous (Phosphate-P) Total pesticides Sand-Silt-Clay ratio
The following parameters will be measured: pH
TOC
Conductivity
Nitrates (Nitrogen)
Potassium
Calcium
Sodium
Carbon
Oil & grease
Mercury
Chromium
Cadmium
Lead
Phosphorous (Phosphate-P)
Total pesticides
Sand-Silt-Clay ratio

11. FOR PRIMARY PRODUCTIVITY MONITORING
AS A PART OF AQUATIC HEALTH MONITORING
The chlorophyll pigment by recording the optical density
at 750, 664, 647 and 630 nm with the help of spectrophotometer
as per the following expressions:
Chl a = OD664 – 1.54 OD OD630
Chl b = OD647 – 5.43 OD OD630
Chl c = OD630 – 1.67 OD OD647

12. Results (WATER QUALITY)
pH of the lake water is alkaline and varies between 8.2 to 8.8 with an average of 8.6 collected from surface water and different depths. (0.8 m – 4.0 m).
Average DO is around 8.5 ( ) mg/l. COD value is above limit (Average 15.3 mg/l) varying between mg/l. BOD values are also above the 3 mg/l level ranging between 3.5 to 6.4.
Faecal coliform organisms are between (1358 to 7803 MPN/100ml).
lead (Pb – mg/l), zinc (Zn – 0.10 – 0.14 mg/l) and mercury (Hg – 0.05 mg/l) particularly underneath the Lake Gardens connector Bridge and in the areas where people are inhabiting and using the lake water for cleaning and bathing which are above the desirable limits and needs to treated. This is largely due to immersion of God/Goddess idol and throwing other puja waste in or near the water course. (Reports enclosed. Sampling and Test Analyses by Good Earth Enviro Care Laboratory, affiliated to West Bengal Pollution Control Board).

13. Sediment Analysis report
The Sediment analyses provides similar trend where pH varies between 7.4 – 8.2,
TOC (9.9 – 17.5),
Faecal coliform ( MPN/g),
Aresenic ( – mg/g), Zinc (0.027 – mg/g), Lead – mg/g) and Mercury ( mg/g) makes the water of Rabindra Saravar lake unsuitable and unhealthy for the growth of fishes and other micro and macro organisms.