1. CHROMATOGRAPHY AP BIO LAB #4A
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2. PLANT PIGMENTS .

3. CHROMATOGRAPHY USE PENCIL not pen, to mark starting line !!!!!
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4. ADD PLANT PIGMENTS
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5. PLACE IN JAR IMPORTANT: Make sure tip of paper is touching solvent
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IMPORTANT: Make sure tip of paper
is touching solvent
Make sure pigment line
is ABOVE solvent
Put lid on jar !!!!!!!!

6. Important Don’t let solvent front run off end of paper !

7. Marker Chromatography
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8. Distance pigment migrated Distance solvent front migrated Rf =
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9. PLANT PIGMENTS Carotene is orange yellow Xanthophyll is lemon yellow
Chlorophyll a is blue green
Chlorophyll b is yellow green.
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10. Factors that affect movement of molecules during chromatography
Solubility
___________of molecule in solvent used
(better it dissolves… farther it will move)
SOLVENT used:
Chlorophyll:
9:1 Petroleum ether : acetone
Markers: water

11. Factors that affect movement of molecules during chromatography
Size
2. _________of molecule
(bigger molecules
move slower)

12. Factors that affect movement of molecules during chromatography
3. _______________________ used
(EX: Attraction of molecules to paper)
Attraction to media
Lots of different kinds of chromatography

13. Factors that affect movement of molecules during chromatography
Time
4. __________allowed to run
Longer time =
More separation

14. CHLOROPHYLL FLUORESCENCE
Image from Biology AP Edition by Campbell & Reece published by Pearson Benjamin Cummings