1. Supplemental PowerPoint Slides
Downregulation of Mir-27b Is Involved In Loss of Type II Collagen By Directly Targeting Matrix Metalloproteinase 13 (MMP13) in Human Intervertebral Disc Degeneration
Hao-ran Li, MD, Qing Cui, MD, Zhan-yin Dong, MD, Jian-hua Zhang, MD, Hai-qing Li, MD, and Ling Zhao, MD
The Department of Spine Surgery, Cangzhou hospital of integrated traditional and western medicine, Cangzhou, Hebei, China
Copyright © 2015 Lippincott Williams & Wilkins. Unauthorized commercial reproduction of this slide is prohibited

2. a b d c e
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3. hsa-miR-27b 3' CGUCUUGAAUCGGUGACACUU 5'
MMP13 3'UTR WT '...GGAGAAAGCUUGGUU-CUGUGAAC 3'
(seed sequence, position )
MMP13 3'UTR MUT 5'...GGAGAAAGCUUGGUU-CACAGUAC 3'
MMP13
GAPDH
Untreated
Scramble
miR-27b mimic d a b c
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4. a b c d
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5. We identified that miR-27b was lower in human degenerative NP tissues and that its level was associated with disc degeneration grade.
The downregulation of miR-27b induces type II collagen loss by directly targeting MMP13, leading to the development of IDD.
Strategies to maintain the expression or to prevent the repression of miR-27b have the potential to become a possible therapeutic strategy for human IDD.
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