1. Approach
Extract all annotated exons (Refseq and KnownGene) in region plus buffer (200KB up & down stream.
Extract all conserved elements with conservation score no lower than the average exon conservation score.
Generate a primer tailing of the target elements tailored to the sequencing read size provided by the sequencing technology used.

2. DISC1/TSNAX Region Size: 500KB 22 Exons 4KB bases
300 non exonic conserved elements spanning 20KB
Approx number of primer pairs
200 base read length 500
800 base read 400

4. DISC1/TSNAX

5. DISC1/TSNAX Exon Size Distribution

6. Neuregulin 1 Largest Isoform 1.1 MB 17 annotated exons spanning 6KB
850 non exonic conserved elements spanning 73KB.
Approx number of primer pairs
200 base read length 1500
800 base read 1000

7. NRG1

8. NRG1 Exons