1. Calcium Chloride A New Tool for Chronic Poverty
Ruth Steinberger, Co-CEO Spay FIRST!

2. Thank you to Elaine Lissner, Director of Parsemus Foundation for making our work with calcium chloride possible. Thank you to researchers and visionaries Billy Clay, DVM, HW Haney, DVM and Charles Helwig, DVM for making it possible to collect data with the goal of helping animals in chronic poverty and to Peter Denooij, DVM for his vision of delivering services to animals where it has previously been difficult to do so.

3. Worldwide dogs and cats exist within a broad spectrum of care…
Some dogs and cats get all necessary veterinary care;
Many get some veterinary care;
Most get none.
75% of dogs worldwide receive no veterinary care

4. Main roadblocks to traditional services…
Extreme poverty, little to no funds for supplies, equipment or appropriate space.
Massive numbers of animals need spay/neuter in order to have an effect on population.
Shortage of human resources (veterinary services to meet the needs).
Remote locations, difficult logistics.

6. Why new tools?
Provide greater volume. Maximize the use of surgeons, time and money.

7. Our goal is not to replace what works well, but instead to expand capacity to prevent unwanted litters born to animals that will not otherwise be altered.
In order to do all that, we need to dramatically decrease costs and eliminate roadblocks to getting animals altered in high-risk circumstances.

8. The overwhelming numbers of animals requires cost effective programs.

9. Temporary space and the high cost for surgical supplies can limit the numbers.

10. Calcium chloride provides a large volume of sterilizations of male dogs and cats at a fraction of the cost, and without the necessary equipment, of surgery and it can even be used in very challenging settings.

11. In the case of chronic poverty, remote areas, long distances and other challenges, we must meet the issues with easy, low cost, non-surgical options that have benefits equal to castration.

12. Costs: Calcium Chloride in Ethyl Alcohol
A separate needle and syringe used for each testicle
Sedation and pain medication used
Chlorhexidine
Gauze
Exam gloves
Total under US $ 1.00

13. HOW DOES IT WORK? BILLY CLAY MS, DVM, DIPLOMATE
AMERICAN BOARD OF VETERINARY TOXICOLOGY
STILLWATER, OK USA

14. CALCIUM CHLORIDE DIHYDRATE
A 20 % SOLUTION PREPARED WITH STERILE ETHYL ALCOHOL
INJECTED INTO THE CENTER OF EACH TESTICLE WITH SUFFICIENT PRESSURE TO AVOID LEAKAGE INTO THE SCROTUM
THE HYPERTONIC SOLUTION EXERTS OSMOTIC PRESSURE ACROSS CELLULAR MEMBRANES

15. CALCIUM CHLORIDE DIHYDRATE
FLUID TRAVERSES THE MEMBRANES OF CELLS AS WELL AS EXTRAVASCULARLY RESULTING IN SWELLING OF EXTRACELLULAR TISSUES.
THE INTEGRITY OF CELLS ARE LOST RESULTING IN DEATH AND NECROSIS- AND FINALLY FIBROSIS

16. Left Testicle measured 32 mm
Right Testicle measured 25 mm

17. Scrotum is 1.5x TO 2.0x NORMAL

19. Left Testicle measured 26 mm (-6)
Right Testicle measured 20 mm (-5)

20. Castration after 30 days

21. Study in Collaboration with Spay First Pathology by Keith Bailey (OSU Lab)
Testicular and Epididymis pathology was performed on 14 dogs 3-30 days post injection
Testes showed inflammatory response with mineralization, fibrosis and tubular degeneration.
Epididymides showed similar inflammation, mineralization and fibroplasia.

22. Leakage into the Scrotum?
A similar necrosis will occur with erosion to the skin surface in some.
Those lesions will heal in about 30 days.

23. Calcium Chloride A New Tool for Underserved Areas
Presenter H. W. Haney, DVM, Cache, Oklahoma, USA

24. What are “intratesticular injections of calcium chloride?”
Calcium chloride for this purpose contains 4 grams calcium chloride dihydrate in 20 ml of ethyl alcohol. It is made of pharmaceutical grade products.
It reduces testosterone significantly and the testicles atrophy. .

26. Our trials
Existing data since 1978 on dogs, in-depth publications in 2007, 2011 Confirmed by HCG challenged testosterone tests funded by Spay FIRST! through generous grants from Parsemus Foundation. Pathology continuing on samples.

27. Dogs injected and challenged with HCG at 30 days. Below 0
Dogs injected and challenged with HCG at 30 days. Below 0.5 ng/ml of testosterone is a castrated dog. Up to 9.0 ng/ml is within the normal range. Testicles atrophied.
Injected on
Jan 31, 2014
30 day HCG challenge Feb 28, 2014
Dog 1
0.004
Dog 2
Dog 3
0.594

28. Outcomes Testosterone near or below the level of a castrated dog.
Eliminated libido (major safety factor).
Reduced costs dramatically
Permanent atrophy of testicles

29. Supplies

30. Tool for measurement
Gauze and soap
Syringes and extra 23 g needles in 1’ and 1.5”
Calcium chloride
Ethyl alcohol

31. Measuring each testicle

32. Write the size of the testicle for measuring the correct dose.

34. It is vital that no calcium chloride enter the scrotum
It is vital that no calcium chloride enter the scrotum. Use of separate, clean needle for each testicle is necessary. Insert clean needle into bottle

35. Pull correct dose into sterile syringe

36. Replace sterile needle on the syringe with calcium chloride and repeat for second testicle.

37. You will have two syringes with the correct dose of calcium chloride in each. A sterile needle will be attached to each syringe before injection.
Any needle that has been in the calcium chloride will be discarded.

39. One wipe with chlorhexidine solution.

40. Use the correct length needle to reach center of testicle
Use the correct length needle to reach center of testicle. Have 1” and 1.5 “ needles on hand.

43. Remove needle very slowly and pinch off testicle

44. Identification Tattoo each dog Ear tattoo if appropriate
Microchip if desired
Use a system that works in your area

45. Calcium Chloride Distribution
Peter P. Denooij, DVM

46. Calcium Chloride Distribution
Bottle contains 4 grams of
Calcium Chloride Dihydrate
Sterile
Add 20 ml of 95% Ethyl Alcohol
Results in 20 ml of 20% solution of CaCl2

47. Calcium Chloride Distribution

48. Calcium Chloride Distribution
1 box contains enough CaCl2 to produce
1000 ml of 20% calcium chloride solution

49. Calcium Chloride Distribution
Veterinarians only

50. Calcium Chloride Distribution
Ordering Process
Place order with CaClCa Inc.
Receive Paypal Invoice
Includes cost of bottles, packing, handling, shipping and service charges
Pay invoice through Paypal
Shipping via DHL Express
You will receive shipping progress by

51. For information:
All veterinary inquiries regarding CaCl will be answered by a licensed veterinarian.